Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06505 and RR42_RS06510 are separated by 149 nucleotides RR42_RS06510 and RR42_RS06515 are separated by 55 nucleotides RR42_RS06515 and RR42_RS06520 are separated by 344 nucleotides
RR42_RS06505: RR42_RS06505 - hypothetical protein, at 1,423,701 to 1,424,591
_RS06505
RR42_RS06510: RR42_RS06510 - hypothetical protein, at 1,424,741 to 1,425,202
_RS06510
RR42_RS06515: RR42_RS06515 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 1,425,258 to 1,426,943
_RS06515
RR42_RS06520: RR42_RS06520 - short-chain dehydrogenase, at 1,427,288 to 1,428,073
_RS06520
Position (kb)
1425
1426
1427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1424.540 kb on + strand at 1424.540 kb on + strand at 1424.540 kb on + strand at 1424.540 kb on + strand at 1424.540 kb on + strand at 1424.540 kb on + strand at 1424.540 kb on + strand at 1424.541 kb on - strand at 1424.544 kb on + strand at 1424.544 kb on + strand at 1424.545 kb on - strand at 1424.545 kb on - strand at 1424.545 kb on - strand at 1424.751 kb on + strand at 1424.751 kb on + strand at 1424.752 kb on - strand at 1424.827 kb on + strand, within RR42_RS06510 at 1425.155 kb on + strand, within RR42_RS06510 at 1425.155 kb on + strand, within RR42_RS06510 at 1425.155 kb on + strand, within RR42_RS06510 at 1425.156 kb on - strand at 1425.156 kb on - strand at 1425.165 kb on + strand at 1425.166 kb on - strand at 1425.166 kb on - strand at 1425.259 kb on + strand at 1425.259 kb on + strand at 1425.259 kb on + strand at 1425.259 kb on + strand at 1425.259 kb on + strand at 1425.260 kb on - strand at 1425.260 kb on - strand at 1425.260 kb on - strand at 1425.260 kb on - strand at 1425.260 kb on - strand at 1425.260 kb on - strand at 1425.271 kb on + strand at 1425.272 kb on - strand at 1427.007 kb on - strand at 1427.115 kb on - strand at 1427.189 kb on + strand at 1427.190 kb on - strand at 1427.277 kb on + strand at 1427.288 kb on - strand at 1427.563 kb on + strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.564 kb on - strand, within RR42_RS06520 at 1427.638 kb on + strand, within RR42_RS06520 at 1427.792 kb on + strand, within RR42_RS06520 at 1427.792 kb on + strand, within RR42_RS06520 at 1427.793 kb on - strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520 at 1427.888 kb on + strand, within RR42_RS06520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,424,540 + -0.6 1,424,540 + +0.2 1,424,540 + -0.7 1,424,540 + -0.0 1,424,540 + -0.8 1,424,540 + +0.4 1,424,540 + +0.1 1,424,541 - -2.9 1,424,544 + -1.0 1,424,544 + +0.8 1,424,545 - -0.1 1,424,545 - -0.6 1,424,545 - -0.8 1,424,751 + +0.3 1,424,751 + +0.1 1,424,752 - -0.4 1,424,827 + RR42_RS06510 0.19 +1.6 1,425,155 + RR42_RS06510 0.90 +0.6 1,425,155 + RR42_RS06510 0.90 -1.0 1,425,155 + RR42_RS06510 0.90 +0.5 1,425,156 - +0.3 1,425,156 - -1.0 1,425,165 + -0.7 1,425,166 - +0.1 1,425,166 - +0.7 1,425,259 + +0.4 1,425,259 + -0.8 1,425,259 + -0.2 1,425,259 + +1.3 1,425,259 + -0.0 1,425,260 - +0.6 1,425,260 - -0.5 1,425,260 - +1.6 1,425,260 - +0.3 1,425,260 - -1.2 1,425,260 - -0.8 1,425,271 + -1.0 1,425,272 - -1.4 1,427,007 - -0.5 1,427,115 - +1.3 1,427,189 + -0.2 1,427,190 - -1.9 1,427,277 + -0.1 1,427,288 - +0.9 1,427,563 + RR42_RS06520 0.35 +0.2 1,427,564 - RR42_RS06520 0.35 -0.2 1,427,564 - RR42_RS06520 0.35 -0.3 1,427,564 - RR42_RS06520 0.35 -0.6 1,427,638 + RR42_RS06520 0.45 +0.7 1,427,792 + RR42_RS06520 0.64 -0.3 1,427,792 + RR42_RS06520 0.64 +0.8 1,427,793 - RR42_RS06520 0.64 +0.4 1,427,888 + RR42_RS06520 0.76 -1.3 1,427,888 + RR42_RS06520 0.76 +1.8 1,427,888 + RR42_RS06520 0.76 -0.4
Or see this region's nucleotide sequence