Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06285 and RR42_RS06290 are separated by 515 nucleotides RR42_RS06290 and RR42_RS06295 are separated by 9 nucleotides
RR42_RS06285: RR42_RS06285 - DNA mismatch repair protein MutS, at 1,377,538 to 1,380,180
_RS06285
RR42_RS06290: RR42_RS06290 - universal stress protein, at 1,380,696 to 1,381,121
_RS06290
RR42_RS06295: RR42_RS06295 - amino acid transporter, at 1,381,131 to 1,383,098
_RS06295
Position (kb)
1380
1381
1382 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1379.822 kb on + strand, within RR42_RS06285 at 1379.822 kb on + strand, within RR42_RS06285 at 1379.822 kb on + strand, within RR42_RS06285 at 1379.822 kb on + strand, within RR42_RS06285 at 1379.822 kb on + strand, within RR42_RS06285 at 1379.823 kb on - strand, within RR42_RS06285 at 1379.823 kb on - strand, within RR42_RS06285 at 1379.823 kb on - strand, within RR42_RS06285 at 1379.823 kb on - strand, within RR42_RS06285 at 1379.826 kb on + strand, within RR42_RS06285 at 1379.827 kb on - strand, within RR42_RS06285 at 1379.981 kb on + strand at 1379.982 kb on - strand at 1380.107 kb on + strand at 1380.108 kb on - strand at 1380.108 kb on - strand at 1380.125 kb on + strand at 1380.126 kb on - strand at 1380.126 kb on - strand at 1380.241 kb on + strand at 1380.293 kb on + strand at 1380.592 kb on + strand at 1380.593 kb on - strand at 1380.618 kb on + strand at 1380.618 kb on + strand at 1380.618 kb on + strand at 1380.619 kb on - strand at 1380.619 kb on - strand at 1380.619 kb on - strand at 1380.620 kb on + strand at 1380.620 kb on + strand at 1380.621 kb on - strand at 1380.645 kb on - strand at 1380.645 kb on - strand at 1380.793 kb on + strand, within RR42_RS06290 at 1380.794 kb on - strand, within RR42_RS06290 at 1380.939 kb on + strand, within RR42_RS06290 at 1380.939 kb on + strand, within RR42_RS06290 at 1380.939 kb on + strand, within RR42_RS06290 at 1380.939 kb on + strand, within RR42_RS06290 at 1380.939 kb on + strand, within RR42_RS06290 at 1380.939 kb on + strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1380.940 kb on - strand, within RR42_RS06290 at 1381.084 kb on + strand at 1381.084 kb on + strand at 1381.085 kb on - strand at 1381.099 kb on + strand at 1381.099 kb on + strand at 1381.137 kb on + strand at 1381.137 kb on + strand at 1381.137 kb on + strand at 1381.138 kb on - strand at 1381.138 kb on - strand at 1381.138 kb on - strand at 1381.179 kb on + strand at 1381.180 kb on - strand at 1381.390 kb on + strand, within RR42_RS06295 at 1381.390 kb on + strand, within RR42_RS06295 at 1381.391 kb on - strand, within RR42_RS06295 at 1381.391 kb on - strand, within RR42_RS06295 at 1381.450 kb on + strand, within RR42_RS06295 at 1381.684 kb on + strand, within RR42_RS06295 at 1381.867 kb on + strand, within RR42_RS06295 at 1381.867 kb on + strand, within RR42_RS06295 at 1381.868 kb on - strand, within RR42_RS06295 at 1381.868 kb on - strand, within RR42_RS06295 at 1381.868 kb on - strand, within RR42_RS06295 at 1381.921 kb on + strand, within RR42_RS06295 at 1381.922 kb on - strand, within RR42_RS06295 at 1381.922 kb on - strand, within RR42_RS06295 at 1381.922 kb on - strand, within RR42_RS06295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,379,822 + RR42_RS06285 0.86 -0.4 1,379,822 + RR42_RS06285 0.86 -2.4 1,379,822 + RR42_RS06285 0.86 -1.5 1,379,822 + RR42_RS06285 0.86 +0.7 1,379,822 + RR42_RS06285 0.86 -0.4 1,379,823 - RR42_RS06285 0.86 -1.7 1,379,823 - RR42_RS06285 0.86 -0.7 1,379,823 - RR42_RS06285 0.86 -0.1 1,379,823 - RR42_RS06285 0.86 +0.2 1,379,826 + RR42_RS06285 0.87 -0.0 1,379,827 - RR42_RS06285 0.87 +0.5 1,379,981 + -0.5 1,379,982 - -0.3 1,380,107 + -1.3 1,380,108 - +1.2 1,380,108 - -1.2 1,380,125 + -0.4 1,380,126 - -2.0 1,380,126 - -0.3 1,380,241 + -0.5 1,380,293 + -0.8 1,380,592 + -1.0 1,380,593 - -1.5 1,380,618 + -0.3 1,380,618 + +0.8 1,380,618 + +0.3 1,380,619 - -0.1 1,380,619 - +0.2 1,380,619 - -1.0 1,380,620 + +0.9 1,380,620 + -0.9 1,380,621 - +0.1 1,380,645 - -0.4 1,380,645 - -0.8 1,380,793 + RR42_RS06290 0.23 -0.0 1,380,794 - RR42_RS06290 0.23 +0.4 1,380,939 + RR42_RS06290 0.57 +0.4 1,380,939 + RR42_RS06290 0.57 +0.6 1,380,939 + RR42_RS06290 0.57 -0.1 1,380,939 + RR42_RS06290 0.57 -0.1 1,380,939 + RR42_RS06290 0.57 -0.1 1,380,939 + RR42_RS06290 0.57 +4.8 1,380,940 - RR42_RS06290 0.57 -0.0 1,380,940 - RR42_RS06290 0.57 -0.2 1,380,940 - RR42_RS06290 0.57 -1.2 1,380,940 - RR42_RS06290 0.57 -2.0 1,380,940 - RR42_RS06290 0.57 -0.3 1,380,940 - RR42_RS06290 0.57 -0.1 1,380,940 - RR42_RS06290 0.57 -0.3 1,381,084 + -0.3 1,381,084 + +0.9 1,381,085 - -1.0 1,381,099 + +0.2 1,381,099 + -0.7 1,381,137 + -0.8 1,381,137 + +1.1 1,381,137 + -0.9 1,381,138 - +0.1 1,381,138 - +0.3 1,381,138 - -0.4 1,381,179 + -0.5 1,381,180 - +0.5 1,381,390 + RR42_RS06295 0.13 -1.7 1,381,390 + RR42_RS06295 0.13 -1.1 1,381,391 - RR42_RS06295 0.13 -0.7 1,381,391 - RR42_RS06295 0.13 +0.2 1,381,450 + RR42_RS06295 0.16 -0.5 1,381,684 + RR42_RS06295 0.28 -0.4 1,381,867 + RR42_RS06295 0.37 -0.4 1,381,867 + RR42_RS06295 0.37 +0.4 1,381,868 - RR42_RS06295 0.37 -0.1 1,381,868 - RR42_RS06295 0.37 +0.4 1,381,868 - RR42_RS06295 0.37 +1.2 1,381,921 + RR42_RS06295 0.40 +0.9 1,381,922 - RR42_RS06295 0.40 -0.3 1,381,922 - RR42_RS06295 0.40 -0.8 1,381,922 - RR42_RS06295 0.40 +0.6
Or see this region's nucleotide sequence