Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05740 and RR42_RS05745 are separated by 266 nucleotides RR42_RS05745 and RR42_RS05750 are separated by 117 nucleotides RR42_RS05750 and RR42_RS05755 are separated by 105 nucleotides
RR42_RS05740: RR42_RS05740 - ABC transporter substrate-binding protein, at 1,262,869 to 1,263,876
_RS05740
RR42_RS05745: RR42_RS05745 - aminoglycoside phosphotransferase, at 1,264,143 to 1,265,219
_RS05745
RR42_RS05750: RR42_RS05750 - fructose 2,6-bisphosphatase, at 1,265,337 to 1,266,071
_RS05750
RR42_RS05755: RR42_RS05755 - enoyl-CoA hydratase, at 1,266,177 to 1,266,956
_RS05755
Position (kb)
1264
1265
1266 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1263.291 kb on - strand, within RR42_RS05740 at 1263.291 kb on - strand, within RR42_RS05740 at 1263.291 kb on - strand, within RR42_RS05740 at 1263.299 kb on + strand, within RR42_RS05740 at 1263.299 kb on + strand, within RR42_RS05740 at 1263.299 kb on + strand, within RR42_RS05740 at 1263.299 kb on + strand, within RR42_RS05740 at 1263.300 kb on - strand, within RR42_RS05740 at 1263.300 kb on - strand, within RR42_RS05740 at 1263.423 kb on + strand, within RR42_RS05740 at 1263.424 kb on - strand, within RR42_RS05740 at 1263.467 kb on + strand, within RR42_RS05740 at 1263.730 kb on + strand, within RR42_RS05740 at 1263.730 kb on + strand, within RR42_RS05740 at 1263.731 kb on - strand, within RR42_RS05740 at 1263.731 kb on - strand, within RR42_RS05740 at 1263.731 kb on - strand, within RR42_RS05740 at 1263.731 kb on - strand, within RR42_RS05740 at 1263.731 kb on - strand, within RR42_RS05740 at 1263.731 kb on - strand, within RR42_RS05740 at 1263.754 kb on - strand, within RR42_RS05740 at 1263.940 kb on + strand at 1263.941 kb on - strand at 1263.941 kb on - strand at 1264.145 kb on - strand at 1264.304 kb on - strand, within RR42_RS05745 at 1264.348 kb on + strand, within RR42_RS05745 at 1264.349 kb on - strand, within RR42_RS05745 at 1264.352 kb on + strand, within RR42_RS05745 at 1264.352 kb on + strand, within RR42_RS05745 at 1264.353 kb on - strand, within RR42_RS05745 at 1264.361 kb on - strand, within RR42_RS05745 at 1264.361 kb on - strand, within RR42_RS05745 at 1264.577 kb on + strand, within RR42_RS05745 at 1264.577 kb on + strand, within RR42_RS05745 at 1264.577 kb on + strand, within RR42_RS05745 at 1264.578 kb on - strand, within RR42_RS05745 at 1264.578 kb on - strand, within RR42_RS05745 at 1264.601 kb on + strand, within RR42_RS05745 at 1264.601 kb on + strand, within RR42_RS05745 at 1264.602 kb on - strand, within RR42_RS05745 at 1264.666 kb on + strand, within RR42_RS05745 at 1264.666 kb on + strand, within RR42_RS05745 at 1264.667 kb on - strand, within RR42_RS05745 at 1264.667 kb on - strand, within RR42_RS05745 at 1264.702 kb on + strand, within RR42_RS05745 at 1264.702 kb on + strand, within RR42_RS05745 at 1264.702 kb on + strand, within RR42_RS05745 at 1264.702 kb on + strand, within RR42_RS05745 at 1264.703 kb on - strand, within RR42_RS05745 at 1264.744 kb on + strand, within RR42_RS05745 at 1264.745 kb on - strand, within RR42_RS05745 at 1264.789 kb on + strand, within RR42_RS05745 at 1264.790 kb on - strand, within RR42_RS05745 at 1264.790 kb on - strand, within RR42_RS05745 at 1264.790 kb on - strand, within RR42_RS05745 at 1264.870 kb on + strand, within RR42_RS05745 at 1264.912 kb on + strand, within RR42_RS05745 at 1264.912 kb on + strand, within RR42_RS05745 at 1264.913 kb on - strand, within RR42_RS05745 at 1264.913 kb on - strand, within RR42_RS05745 at 1264.913 kb on - strand, within RR42_RS05745 at 1265.032 kb on + strand, within RR42_RS05745 at 1265.032 kb on + strand, within RR42_RS05745 at 1265.033 kb on - strand, within RR42_RS05745 at 1265.033 kb on - strand, within RR42_RS05745 at 1265.266 kb on + strand at 1265.267 kb on - strand at 1265.267 kb on - strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.320 kb on - strand at 1265.320 kb on - strand at 1265.320 kb on - strand at 1265.320 kb on - strand at 1265.338 kb on + strand at 1265.374 kb on + strand at 1265.375 kb on - strand at 1265.440 kb on + strand, within RR42_RS05750 at 1266.010 kb on + strand at 1266.010 kb on + strand at 1266.011 kb on - strand at 1266.037 kb on + strand at 1266.038 kb on - strand at 1266.178 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,263,291 - RR42_RS05740 0.42 -1.4 1,263,291 - RR42_RS05740 0.42 +0.2 1,263,291 - RR42_RS05740 0.42 -1.0 1,263,299 + RR42_RS05740 0.43 -0.2 1,263,299 + RR42_RS05740 0.43 +0.0 1,263,299 + RR42_RS05740 0.43 -2.6 1,263,299 + RR42_RS05740 0.43 -0.6 1,263,300 - RR42_RS05740 0.43 -0.1 1,263,300 - RR42_RS05740 0.43 -0.4 1,263,423 + RR42_RS05740 0.55 -1.7 1,263,424 - RR42_RS05740 0.55 +1.3 1,263,467 + RR42_RS05740 0.59 -0.7 1,263,730 + RR42_RS05740 0.85 -0.3 1,263,730 + RR42_RS05740 0.85 -0.6 1,263,731 - RR42_RS05740 0.86 -0.2 1,263,731 - RR42_RS05740 0.86 +0.5 1,263,731 - RR42_RS05740 0.86 +0.8 1,263,731 - RR42_RS05740 0.86 -0.0 1,263,731 - RR42_RS05740 0.86 -1.0 1,263,731 - RR42_RS05740 0.86 -0.1 1,263,754 - RR42_RS05740 0.88 -0.0 1,263,940 + -0.5 1,263,941 - -0.7 1,263,941 - +0.5 1,264,145 - -0.0 1,264,304 - RR42_RS05745 0.15 +0.1 1,264,348 + RR42_RS05745 0.19 +0.6 1,264,349 - RR42_RS05745 0.19 +0.5 1,264,352 + RR42_RS05745 0.19 +0.0 1,264,352 + RR42_RS05745 0.19 -0.1 1,264,353 - RR42_RS05745 0.19 +1.3 1,264,361 - RR42_RS05745 0.20 -0.2 1,264,361 - RR42_RS05745 0.20 -0.3 1,264,577 + RR42_RS05745 0.40 -1.4 1,264,577 + RR42_RS05745 0.40 -0.2 1,264,577 + RR42_RS05745 0.40 -0.0 1,264,578 - RR42_RS05745 0.40 -0.0 1,264,578 - RR42_RS05745 0.40 +0.4 1,264,601 + RR42_RS05745 0.43 +0.0 1,264,601 + RR42_RS05745 0.43 -0.1 1,264,602 - RR42_RS05745 0.43 +0.0 1,264,666 + RR42_RS05745 0.49 +1.1 1,264,666 + RR42_RS05745 0.49 +0.8 1,264,667 - RR42_RS05745 0.49 +0.4 1,264,667 - RR42_RS05745 0.49 +1.2 1,264,702 + RR42_RS05745 0.52 +0.6 1,264,702 + RR42_RS05745 0.52 +1.3 1,264,702 + RR42_RS05745 0.52 -0.0 1,264,702 + RR42_RS05745 0.52 +0.4 1,264,703 - RR42_RS05745 0.52 +3.6 1,264,744 + RR42_RS05745 0.56 -0.3 1,264,745 - RR42_RS05745 0.56 +0.5 1,264,789 + RR42_RS05745 0.60 +0.7 1,264,790 - RR42_RS05745 0.60 +0.4 1,264,790 - RR42_RS05745 0.60 +1.4 1,264,790 - RR42_RS05745 0.60 +0.6 1,264,870 + RR42_RS05745 0.68 +1.3 1,264,912 + RR42_RS05745 0.71 +0.3 1,264,912 + RR42_RS05745 0.71 -0.0 1,264,913 - RR42_RS05745 0.71 +0.4 1,264,913 - RR42_RS05745 0.71 -0.4 1,264,913 - RR42_RS05745 0.71 -0.5 1,265,032 + RR42_RS05745 0.83 +0.4 1,265,032 + RR42_RS05745 0.83 -2.3 1,265,033 - RR42_RS05745 0.83 +0.8 1,265,033 - RR42_RS05745 0.83 +0.9 1,265,266 + -0.0 1,265,267 - -0.5 1,265,267 - +0.2 1,265,319 + +0.1 1,265,319 + +1.9 1,265,319 + +1.1 1,265,319 + +0.3 1,265,319 + +0.2 1,265,319 + -1.1 1,265,319 + +0.6 1,265,320 - +0.1 1,265,320 - -1.3 1,265,320 - -0.5 1,265,320 - +0.1 1,265,338 + -0.7 1,265,374 + -0.3 1,265,375 - +0.2 1,265,440 + RR42_RS05750 0.14 +0.6 1,266,010 + -0.8 1,266,010 + -1.5 1,266,011 - -1.8 1,266,037 + -1.0 1,266,038 - -0.2 1,266,178 + -0.6
Or see this region's nucleotide sequence