Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05410 and RR42_RS05415 overlap by 4 nucleotides RR42_RS05415 and RR42_RS05420 are separated by 193 nucleotides RR42_RS05420 and RR42_RS05425 are separated by 2 nucleotides RR42_RS05425 and RR42_RS05430 overlap by 4 nucleotides
RR42_RS05410: RR42_RS05410 - acetyltransferase, at 1,195,339 to 1,196,316
_RS05410
RR42_RS05415: RR42_RS05415 - membrane protein, at 1,196,313 to 1,196,879
_RS05415
RR42_RS05420: RR42_RS05420 - membrane protein, at 1,197,073 to 1,197,852
_RS05420
RR42_RS05425: RR42_RS05425 - membrane protein, at 1,197,855 to 1,198,289
_RS05425
RR42_RS05430: RR42_RS05430 - DNA-directed RNA polymerase sigma-70 factor, at 1,198,286 to 1,198,852
_RS05430
Position (kb)
1197
1198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1196.136 kb on + strand, within RR42_RS05410 at 1196.427 kb on + strand, within RR42_RS05415 at 1196.428 kb on - strand, within RR42_RS05415 at 1196.428 kb on - strand, within RR42_RS05415 at 1196.512 kb on + strand, within RR42_RS05415 at 1196.626 kb on + strand, within RR42_RS05415 at 1196.627 kb on - strand, within RR42_RS05415 at 1196.713 kb on + strand, within RR42_RS05415 at 1196.713 kb on + strand, within RR42_RS05415 at 1196.714 kb on - strand, within RR42_RS05415 at 1196.752 kb on - strand, within RR42_RS05415 at 1196.995 kb on + strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1196.996 kb on - strand at 1197.416 kb on + strand, within RR42_RS05420 at 1197.417 kb on - strand, within RR42_RS05420 at 1197.417 kb on - strand, within RR42_RS05420 at 1197.471 kb on - strand, within RR42_RS05420 at 1197.857 kb on - strand at 1197.857 kb on - strand at 1197.876 kb on + strand at 1197.876 kb on + strand at 1197.877 kb on - strand at 1197.899 kb on - strand, within RR42_RS05425 at 1197.982 kb on + strand, within RR42_RS05425 at 1197.982 kb on + strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1197.983 kb on - strand, within RR42_RS05425 at 1198.406 kb on - strand, within RR42_RS05430 at 1198.567 kb on + strand, within RR42_RS05430 at 1198.619 kb on - strand, within RR42_RS05430 at 1198.626 kb on - strand, within RR42_RS05430 at 1198.667 kb on + strand, within RR42_RS05430 at 1198.668 kb on - strand, within RR42_RS05430 at 1198.832 kb on + strand at 1198.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,196,136 + RR42_RS05410 0.81 -0.4 1,196,427 + RR42_RS05415 0.20 +2.0 1,196,428 - RR42_RS05415 0.20 +0.5 1,196,428 - RR42_RS05415 0.20 -0.9 1,196,512 + RR42_RS05415 0.35 -0.0 1,196,626 + RR42_RS05415 0.55 -1.6 1,196,627 - RR42_RS05415 0.55 -0.0 1,196,713 + RR42_RS05415 0.71 -0.7 1,196,713 + RR42_RS05415 0.71 -0.0 1,196,714 - RR42_RS05415 0.71 +0.5 1,196,752 - RR42_RS05415 0.77 -1.0 1,196,995 + +0.2 1,196,996 - -1.3 1,196,996 - +0.4 1,196,996 - -1.0 1,197,416 + RR42_RS05420 0.44 -3.3 1,197,417 - RR42_RS05420 0.44 -2.6 1,197,417 - RR42_RS05420 0.44 -1.9 1,197,471 - RR42_RS05420 0.51 -0.9 1,197,857 - -1.1 1,197,857 - -1.7 1,197,876 + -1.9 1,197,876 + +0.3 1,197,877 - -3.5 1,197,899 - RR42_RS05425 0.10 +0.3 1,197,982 + RR42_RS05425 0.29 -0.7 1,197,982 + RR42_RS05425 0.29 -1.4 1,197,983 - RR42_RS05425 0.29 -1.9 1,197,983 - RR42_RS05425 0.29 -0.7 1,197,983 - RR42_RS05425 0.29 -2.0 1,197,983 - RR42_RS05425 0.29 -1.0 1,197,983 - RR42_RS05425 0.29 -0.4 1,197,983 - RR42_RS05425 0.29 -1.0 1,197,983 - RR42_RS05425 0.29 -1.0 1,197,983 - RR42_RS05425 0.29 -1.9 1,198,406 - RR42_RS05430 0.21 +1.2 1,198,567 + RR42_RS05430 0.50 +0.9 1,198,619 - RR42_RS05430 0.59 +1.3 1,198,626 - RR42_RS05430 0.60 -0.4 1,198,667 + RR42_RS05430 0.67 +1.3 1,198,668 - RR42_RS05430 0.67 -1.3 1,198,832 + +1.6 1,198,832 + -0.8
Or see this region's nucleotide sequence