Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05360 and RR42_RS05365 overlap by 16 nucleotides RR42_RS05365 and RR42_RS05370 overlap by 2 nucleotides RR42_RS05370 and RR42_RS05375 are separated by 24 nucleotides RR42_RS05375 and RR42_RS05380 are separated by 38 nucleotides
RR42_RS05360: RR42_RS05360 - membrane protein, at 1,186,341 to 1,186,958
_RS05360
RR42_RS05365: RR42_RS05365 - hypothetical protein, at 1,186,943 to 1,187,275
_RS05365
RR42_RS05370: RR42_RS05370 - ABC transporter permease, at 1,187,274 to 1,189,817
_RS05370
RR42_RS05375: RR42_RS05375 - hemoglobin-like protein, at 1,189,842 to 1,190,279
_RS05375
RR42_RS05380: RR42_RS05380 - membrane protein, at 1,190,318 to 1,190,935
_RS05380
Position (kb)
1187
1188
1189
1190 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1186.280 kb on + strand at 1186.284 kb on - strand at 1186.296 kb on + strand at 1186.345 kb on + strand at 1186.346 kb on - strand at 1186.346 kb on - strand at 1186.346 kb on - strand at 1186.399 kb on - strand at 1186.399 kb on - strand at 1186.399 kb on - strand at 1186.890 kb on + strand, within RR42_RS05360 at 1186.891 kb on - strand, within RR42_RS05360 at 1187.119 kb on + strand, within RR42_RS05365 at 1187.120 kb on - strand, within RR42_RS05365 at 1187.120 kb on - strand, within RR42_RS05365 at 1187.120 kb on - strand, within RR42_RS05365 at 1187.132 kb on + strand, within RR42_RS05365 at 1187.132 kb on + strand, within RR42_RS05365 at 1187.133 kb on - strand, within RR42_RS05365 at 1187.133 kb on - strand, within RR42_RS05365 at 1187.929 kb on - strand, within RR42_RS05370 at 1188.153 kb on + strand, within RR42_RS05370 at 1188.153 kb on + strand, within RR42_RS05370 at 1188.153 kb on + strand, within RR42_RS05370 at 1188.154 kb on - strand, within RR42_RS05370 at 1188.155 kb on + strand, within RR42_RS05370 at 1188.155 kb on + strand, within RR42_RS05370 at 1188.301 kb on - strand, within RR42_RS05370 at 1188.301 kb on - strand, within RR42_RS05370 at 1188.634 kb on + strand, within RR42_RS05370 at 1188.635 kb on - strand, within RR42_RS05370 at 1188.635 kb on - strand, within RR42_RS05370 at 1188.864 kb on - strand, within RR42_RS05370 at 1188.959 kb on + strand, within RR42_RS05370 at 1188.959 kb on + strand, within RR42_RS05370 at 1188.959 kb on + strand, within RR42_RS05370 at 1188.960 kb on - strand, within RR42_RS05370 at 1188.960 kb on - strand, within RR42_RS05370 at 1188.960 kb on - strand, within RR42_RS05370 at 1189.041 kb on + strand, within RR42_RS05370 at 1189.041 kb on + strand, within RR42_RS05370 at 1189.043 kb on + strand, within RR42_RS05370 at 1189.044 kb on - strand, within RR42_RS05370 at 1189.302 kb on + strand, within RR42_RS05370 at 1189.302 kb on + strand, within RR42_RS05370 at 1189.302 kb on + strand, within RR42_RS05370 at 1189.303 kb on - strand, within RR42_RS05370 at 1189.303 kb on - strand, within RR42_RS05370 at 1189.305 kb on - strand, within RR42_RS05370 at 1189.305 kb on - strand, within RR42_RS05370 at 1189.491 kb on + strand, within RR42_RS05370 at 1189.491 kb on + strand, within RR42_RS05370 at 1189.491 kb on + strand, within RR42_RS05370 at 1189.492 kb on - strand, within RR42_RS05370 at 1189.492 kb on - strand, within RR42_RS05370 at 1189.588 kb on - strand at 1189.841 kb on + strand at 1189.841 kb on + strand at 1189.841 kb on + strand at 1189.841 kb on + strand at 1189.842 kb on - strand at 1189.842 kb on - strand at 1189.912 kb on - strand, within RR42_RS05375 at 1189.962 kb on + strand, within RR42_RS05375 at 1189.962 kb on + strand, within RR42_RS05375 at 1189.963 kb on - strand, within RR42_RS05375 at 1189.963 kb on - strand, within RR42_RS05375 at 1190.304 kb on + strand at 1190.305 kb on - strand at 1190.305 kb on - strand at 1190.305 kb on - strand at 1190.305 kb on - strand at 1190.374 kb on - strand at 1190.705 kb on + strand, within RR42_RS05380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,186,280 + +1.2 1,186,284 - -0.7 1,186,296 + +0.4 1,186,345 + -0.1 1,186,346 - +1.3 1,186,346 - +0.4 1,186,346 - -0.7 1,186,399 - +0.1 1,186,399 - -0.1 1,186,399 - -0.6 1,186,890 + RR42_RS05360 0.89 +0.2 1,186,891 - RR42_RS05360 0.89 +1.2 1,187,119 + RR42_RS05365 0.53 -0.3 1,187,120 - RR42_RS05365 0.53 +0.7 1,187,120 - RR42_RS05365 0.53 -0.2 1,187,120 - RR42_RS05365 0.53 -0.8 1,187,132 + RR42_RS05365 0.57 +0.7 1,187,132 + RR42_RS05365 0.57 -0.7 1,187,133 - RR42_RS05365 0.57 +0.8 1,187,133 - RR42_RS05365 0.57 +0.1 1,187,929 - RR42_RS05370 0.26 +1.3 1,188,153 + RR42_RS05370 0.35 +0.2 1,188,153 + RR42_RS05370 0.35 +0.7 1,188,153 + RR42_RS05370 0.35 -0.0 1,188,154 - RR42_RS05370 0.35 -0.5 1,188,155 + RR42_RS05370 0.35 +1.3 1,188,155 + RR42_RS05370 0.35 -1.2 1,188,301 - RR42_RS05370 0.40 +0.0 1,188,301 - RR42_RS05370 0.40 -2.3 1,188,634 + RR42_RS05370 0.53 -0.7 1,188,635 - RR42_RS05370 0.53 -2.7 1,188,635 - RR42_RS05370 0.53 -1.3 1,188,864 - RR42_RS05370 0.62 -0.5 1,188,959 + RR42_RS05370 0.66 -0.3 1,188,959 + RR42_RS05370 0.66 -1.0 1,188,959 + RR42_RS05370 0.66 -0.1 1,188,960 - RR42_RS05370 0.66 -0.7 1,188,960 - RR42_RS05370 0.66 -0.5 1,188,960 - RR42_RS05370 0.66 +0.8 1,189,041 + RR42_RS05370 0.69 +1.7 1,189,041 + RR42_RS05370 0.69 -0.6 1,189,043 + RR42_RS05370 0.70 +1.3 1,189,044 - RR42_RS05370 0.70 +0.2 1,189,302 + RR42_RS05370 0.80 +0.2 1,189,302 + RR42_RS05370 0.80 -0.2 1,189,302 + RR42_RS05370 0.80 -1.0 1,189,303 - RR42_RS05370 0.80 -0.2 1,189,303 - RR42_RS05370 0.80 +1.9 1,189,305 - RR42_RS05370 0.80 +0.7 1,189,305 - RR42_RS05370 0.80 -0.7 1,189,491 + RR42_RS05370 0.87 +0.2 1,189,491 + RR42_RS05370 0.87 -0.6 1,189,491 + RR42_RS05370 0.87 +0.5 1,189,492 - RR42_RS05370 0.87 -1.8 1,189,492 - RR42_RS05370 0.87 -0.1 1,189,588 - -0.5 1,189,841 + +0.3 1,189,841 + -1.2 1,189,841 + -1.0 1,189,841 + -0.1 1,189,842 - +0.3 1,189,842 - -0.0 1,189,912 - RR42_RS05375 0.16 +0.7 1,189,962 + RR42_RS05375 0.27 -0.4 1,189,962 + RR42_RS05375 0.27 -0.2 1,189,963 - RR42_RS05375 0.28 -1.6 1,189,963 - RR42_RS05375 0.28 -0.6 1,190,304 + -0.3 1,190,305 - +0.5 1,190,305 - -0.6 1,190,305 - -0.2 1,190,305 - -0.0 1,190,374 - -1.4 1,190,705 + RR42_RS05380 0.63 -1.0
Or see this region's nucleotide sequence