Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS04920

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS04910 and RR42_RS04915 are separated by 84 nucleotidesRR42_RS04915 and RR42_RS04920 are separated by 257 nucleotidesRR42_RS04920 and RR42_RS04925 are separated by 44 nucleotidesRR42_RS04925 and RR42_RS04930 are separated by 123 nucleotides RR42_RS04910: RR42_RS04910 - guanylate kinase, at 1,088,828 to 1,089,481 _RS04910 RR42_RS04915: RR42_RS04915 - DNA-directed RNA polymerase subunit omega, at 1,089,566 to 1,089,769 _RS04915 RR42_RS04920: RR42_RS04920 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,090,027 to 1,092,444 _RS04920 RR42_RS04925: RR42_RS04925 - transcription elongation factor GreB, at 1,092,489 to 1,093,085 _RS04925 RR42_RS04930: RR42_RS04930 - tRNA-Arg, at 1,093,209 to 1,093,285 _RS04930 Position (kb) 1090 1091 1092 1093Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1089.759 kb on + strandat 1089.804 kb on + strandat 1089.804 kb on + strandat 1089.804 kb on + strandat 1089.831 kb on + strandat 1089.831 kb on + strandat 1089.831 kb on + strandat 1089.831 kb on + strandat 1092.119 kb on + strand, within RR42_RS04920at 1092.331 kb on + strandat 1092.331 kb on + strandat 1092.332 kb on - strandat 1092.332 kb on - strandat 1092.557 kb on + strand, within RR42_RS04925at 1092.600 kb on - strand, within RR42_RS04925at 1092.727 kb on + strand, within RR42_RS04925at 1092.727 kb on + strand, within RR42_RS04925at 1092.728 kb on - strand, within RR42_RS04925at 1092.728 kb on - strand, within RR42_RS04925at 1092.859 kb on + strand, within RR42_RS04925at 1092.860 kb on - strand, within RR42_RS04925at 1092.865 kb on + strand, within RR42_RS04925at 1092.866 kb on - strand, within RR42_RS04925at 1092.976 kb on + strand, within RR42_RS04925at 1092.976 kb on + strand, within RR42_RS04925at 1092.994 kb on + strand, within RR42_RS04925at 1092.994 kb on + strand, within RR42_RS04925at 1092.994 kb on + strand, within RR42_RS04925at 1092.995 kb on - strand, within RR42_RS04925at 1092.995 kb on - strand, within RR42_RS04925at 1093.114 kb on + strandat 1093.115 kb on - strandat 1093.116 kb on + strandat 1093.116 kb on + strandat 1093.116 kb on + strandat 1093.116 kb on + strandat 1093.116 kb on + strandat 1093.117 kb on - strandat 1093.117 kb on - strandat 1093.117 kb on - strandat 1093.117 kb on - strandat 1093.193 kb on - strandat 1093.193 kb on - strandat 1093.373 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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1,089,759 + +0.9
1,089,804 + +0.6
1,089,804 + +2.9
1,089,804 + +2.5
1,089,831 + +2.5
1,089,831 + +0.4
1,089,831 + +1.2
1,089,831 + +1.3
1,092,119 + RR42_RS04920 0.87 +0.9
1,092,331 + +0.3
1,092,331 + +0.1
1,092,332 - -0.9
1,092,332 - +0.6
1,092,557 + RR42_RS04925 0.11 -0.3
1,092,600 - RR42_RS04925 0.19 +1.2
1,092,727 + RR42_RS04925 0.40 +1.1
1,092,727 + RR42_RS04925 0.40 +0.2
1,092,728 - RR42_RS04925 0.40 -1.9
1,092,728 - RR42_RS04925 0.40 +0.2
1,092,859 + RR42_RS04925 0.62 -0.1
1,092,860 - RR42_RS04925 0.62 +1.0
1,092,865 + RR42_RS04925 0.63 +0.4
1,092,866 - RR42_RS04925 0.63 +1.0
1,092,976 + RR42_RS04925 0.82 -0.3
1,092,976 + RR42_RS04925 0.82 +0.1
1,092,994 + RR42_RS04925 0.85 +1.1
1,092,994 + RR42_RS04925 0.85 +0.1
1,092,994 + RR42_RS04925 0.85 -1.5
1,092,995 - RR42_RS04925 0.85 +1.1
1,092,995 - RR42_RS04925 0.85 +2.9
1,093,114 + -1.2
1,093,115 - -0.5
1,093,116 + -0.5
1,093,116 + -0.5
1,093,116 + -0.0
1,093,116 + -1.1
1,093,116 + -0.4
1,093,117 - -0.1
1,093,117 - -0.7
1,093,117 - +0.1
1,093,117 - -1.5
1,093,193 - +1.3
1,093,193 - -1.2
1,093,373 - -0.1

Or see this region's nucleotide sequence