Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04910 and RR42_RS04915 are separated by 84 nucleotides RR42_RS04915 and RR42_RS04920 are separated by 257 nucleotides RR42_RS04920 and RR42_RS04925 are separated by 44 nucleotides RR42_RS04925 and RR42_RS04930 are separated by 123 nucleotides
RR42_RS04910: RR42_RS04910 - guanylate kinase, at 1,088,828 to 1,089,481
_RS04910
RR42_RS04915: RR42_RS04915 - DNA-directed RNA polymerase subunit omega, at 1,089,566 to 1,089,769
_RS04915
RR42_RS04920: RR42_RS04920 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,090,027 to 1,092,444
_RS04920
RR42_RS04925: RR42_RS04925 - transcription elongation factor GreB, at 1,092,489 to 1,093,085
_RS04925
RR42_RS04930: RR42_RS04930 - tRNA-Arg, at 1,093,209 to 1,093,285
_RS04930
Position (kb)
1090
1091
1092
1093 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1089.759 kb on + strand at 1089.804 kb on + strand at 1089.804 kb on + strand at 1089.804 kb on + strand at 1089.831 kb on + strand at 1089.831 kb on + strand at 1089.831 kb on + strand at 1089.831 kb on + strand at 1092.119 kb on + strand, within RR42_RS04920 at 1092.331 kb on + strand at 1092.331 kb on + strand at 1092.332 kb on - strand at 1092.332 kb on - strand at 1092.557 kb on + strand, within RR42_RS04925 at 1092.600 kb on - strand, within RR42_RS04925 at 1092.727 kb on + strand, within RR42_RS04925 at 1092.727 kb on + strand, within RR42_RS04925 at 1092.728 kb on - strand, within RR42_RS04925 at 1092.728 kb on - strand, within RR42_RS04925 at 1092.859 kb on + strand, within RR42_RS04925 at 1092.860 kb on - strand, within RR42_RS04925 at 1092.865 kb on + strand, within RR42_RS04925 at 1092.866 kb on - strand, within RR42_RS04925 at 1092.976 kb on + strand, within RR42_RS04925 at 1092.976 kb on + strand, within RR42_RS04925 at 1092.994 kb on + strand, within RR42_RS04925 at 1092.994 kb on + strand, within RR42_RS04925 at 1092.994 kb on + strand, within RR42_RS04925 at 1092.995 kb on - strand, within RR42_RS04925 at 1092.995 kb on - strand, within RR42_RS04925 at 1093.114 kb on + strand at 1093.115 kb on - strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.117 kb on - strand at 1093.117 kb on - strand at 1093.117 kb on - strand at 1093.117 kb on - strand at 1093.193 kb on - strand at 1093.193 kb on - strand at 1093.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,089,759 + +0.9 1,089,804 + +0.6 1,089,804 + +2.9 1,089,804 + +2.5 1,089,831 + +2.5 1,089,831 + +0.4 1,089,831 + +1.2 1,089,831 + +1.3 1,092,119 + RR42_RS04920 0.87 +0.9 1,092,331 + +0.3 1,092,331 + +0.1 1,092,332 - -0.9 1,092,332 - +0.6 1,092,557 + RR42_RS04925 0.11 -0.3 1,092,600 - RR42_RS04925 0.19 +1.2 1,092,727 + RR42_RS04925 0.40 +1.1 1,092,727 + RR42_RS04925 0.40 +0.2 1,092,728 - RR42_RS04925 0.40 -1.9 1,092,728 - RR42_RS04925 0.40 +0.2 1,092,859 + RR42_RS04925 0.62 -0.1 1,092,860 - RR42_RS04925 0.62 +1.0 1,092,865 + RR42_RS04925 0.63 +0.4 1,092,866 - RR42_RS04925 0.63 +1.0 1,092,976 + RR42_RS04925 0.82 -0.3 1,092,976 + RR42_RS04925 0.82 +0.1 1,092,994 + RR42_RS04925 0.85 +1.1 1,092,994 + RR42_RS04925 0.85 +0.1 1,092,994 + RR42_RS04925 0.85 -1.5 1,092,995 - RR42_RS04925 0.85 +1.1 1,092,995 - RR42_RS04925 0.85 +2.9 1,093,114 + -1.2 1,093,115 - -0.5 1,093,116 + -0.5 1,093,116 + -0.5 1,093,116 + -0.0 1,093,116 + -1.1 1,093,116 + -0.4 1,093,117 - -0.1 1,093,117 - -0.7 1,093,117 - +0.1 1,093,117 - -1.5 1,093,193 - +1.3 1,093,193 - -1.2 1,093,373 - -0.1
Or see this region's nucleotide sequence