Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04700 and RR42_RS04705 are separated by 132 nucleotides RR42_RS04705 and RR42_RS04710 are separated by 108 nucleotides RR42_RS04710 and RR42_RS04715 are separated by 23 nucleotides RR42_RS04715 and RR42_RS04720 are separated by 15 nucleotides
RR42_RS04700: RR42_RS04700 - ribonuclease G, at 1,047,130 to 1,048,596
_RS04700
RR42_RS04705: RR42_RS04705 - septum formation inhibitor Maf, at 1,048,729 to 1,049,334
_RS04705
RR42_RS04710: RR42_RS04710 - 50S rRNA methyltransferase, at 1,049,443 to 1,049,913
_RS04710
RR42_RS04715: RR42_RS04715 - ribosome silencing factor RsfS, at 1,049,937 to 1,050,695
_RS04715
RR42_RS04720: RR42_RS04720 - nicotinate-nucleotide adenylyltransferase, at 1,050,711 to 1,051,481
_RS04720
Position (kb)
1049
1050 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1048.472 kb on + strand at 1048.472 kb on + strand at 1048.473 kb on - strand at 1048.597 kb on - strand at 1048.800 kb on - strand, within RR42_RS04705 at 1048.850 kb on + strand, within RR42_RS04705 at 1048.889 kb on + strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1048.890 kb on - strand, within RR42_RS04705 at 1049.180 kb on + strand, within RR42_RS04705 at 1049.259 kb on - strand, within RR42_RS04705 at 1049.259 kb on - strand, within RR42_RS04705 at 1049.315 kb on + strand at 1049.316 kb on - strand at 1049.316 kb on - strand at 1049.325 kb on + strand at 1049.325 kb on + strand at 1049.325 kb on + strand at 1049.326 kb on - strand at 1049.351 kb on - strand at 1049.400 kb on + strand at 1049.400 kb on + strand at 1049.457 kb on - strand at 1049.772 kb on + strand, within RR42_RS04710 at 1049.772 kb on + strand, within RR42_RS04710 at 1049.773 kb on - strand, within RR42_RS04710 at 1049.773 kb on - strand, within RR42_RS04710 at 1050.173 kb on + strand, within RR42_RS04715 at 1050.403 kb on + strand, within RR42_RS04715 at 1050.403 kb on + strand, within RR42_RS04715 at 1050.404 kb on - strand, within RR42_RS04715 at 1050.404 kb on - strand, within RR42_RS04715 at 1050.406 kb on + strand, within RR42_RS04715 at 1050.407 kb on - strand, within RR42_RS04715 at 1050.407 kb on - strand, within RR42_RS04715 at 1050.622 kb on + strand at 1050.623 kb on - strand at 1050.623 kb on - strand at 1050.689 kb on + strand at 1050.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,048,472 + +1.2 1,048,472 + -1.1 1,048,473 - -1.3 1,048,597 - -0.1 1,048,800 - RR42_RS04705 0.12 -1.1 1,048,850 + RR42_RS04705 0.20 -0.4 1,048,889 + RR42_RS04705 0.26 -0.1 1,048,890 - RR42_RS04705 0.27 -0.2 1,048,890 - RR42_RS04705 0.27 +1.0 1,048,890 - RR42_RS04705 0.27 -0.4 1,048,890 - RR42_RS04705 0.27 +0.4 1,049,180 + RR42_RS04705 0.74 +2.6 1,049,259 - RR42_RS04705 0.87 +0.5 1,049,259 - RR42_RS04705 0.87 -1.0 1,049,315 + -0.8 1,049,316 - -0.3 1,049,316 - -0.4 1,049,325 + -0.3 1,049,325 + +0.1 1,049,325 + -1.1 1,049,326 - +0.3 1,049,351 - +0.4 1,049,400 + -2.6 1,049,400 + -1.2 1,049,457 - +1.3 1,049,772 + RR42_RS04710 0.70 -1.1 1,049,772 + RR42_RS04710 0.70 +0.3 1,049,773 - RR42_RS04710 0.70 -1.9 1,049,773 - RR42_RS04710 0.70 -1.2 1,050,173 + RR42_RS04715 0.31 +0.0 1,050,403 + RR42_RS04715 0.61 -1.1 1,050,403 + RR42_RS04715 0.61 +0.4 1,050,404 - RR42_RS04715 0.62 +0.7 1,050,404 - RR42_RS04715 0.62 -0.0 1,050,406 + RR42_RS04715 0.62 -0.3 1,050,407 - RR42_RS04715 0.62 -1.1 1,050,407 - RR42_RS04715 0.62 +0.1 1,050,622 + -0.7 1,050,623 - -0.7 1,050,623 - +1.3 1,050,689 + -0.4 1,050,690 - -0.1
Or see this region's nucleotide sequence