Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04285 and RR42_RS04290 are separated by 180 nucleotides RR42_RS04290 and RR42_RS04295 are separated by 29 nucleotides RR42_RS04295 and RR42_RS04300 are separated by 252 nucleotides RR42_RS04300 and RR42_RS04305 are separated by 95 nucleotides
RR42_RS04285: RR42_RS04285 - inorganic pyrophosphatase, at 953,239 to 953,766
_RS04285
RR42_RS04290: RR42_RS04290 - cytochrome B561, at 953,947 to 954,501
_RS04290
RR42_RS04295: RR42_RS04295 - hypothetical protein, at 954,531 to 955,790
_RS04295
RR42_RS04300: RR42_RS04300 - nitrogen regulatory protein P-II 1, at 956,043 to 956,381
_RS04300
RR42_RS04305: RR42_RS04305 - membrane protein, at 956,477 to 957,118
_RS04305
Position (kb)
954
955
956 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 953.764 kb on + strand at 953.764 kb on + strand at 953.765 kb on - strand at 953.886 kb on + strand at 953.900 kb on + strand at 953.939 kb on + strand at 953.939 kb on + strand at 953.955 kb on + strand at 953.955 kb on + strand at 953.955 kb on + strand at 953.986 kb on + strand at 954.020 kb on + strand, within RR42_RS04290 at 954.020 kb on + strand, within RR42_RS04290 at 954.021 kb on - strand, within RR42_RS04290 at 954.021 kb on - strand, within RR42_RS04290 at 954.043 kb on + strand, within RR42_RS04290 at 954.242 kb on + strand, within RR42_RS04290 at 954.242 kb on + strand, within RR42_RS04290 at 954.242 kb on + strand, within RR42_RS04290 at 954.243 kb on - strand, within RR42_RS04290 at 954.247 kb on + strand, within RR42_RS04290 at 954.247 kb on + strand, within RR42_RS04290 at 954.248 kb on - strand, within RR42_RS04290 at 954.257 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.416 kb on - strand, within RR42_RS04290 at 954.460 kb on + strand at 954.460 kb on + strand at 954.460 kb on + strand at 954.461 kb on - strand at 954.461 kb on - strand at 954.461 kb on - strand at 954.475 kb on - strand at 954.499 kb on + strand at 954.574 kb on + strand at 954.723 kb on - strand, within RR42_RS04295 at 954.760 kb on + strand, within RR42_RS04295 at 954.761 kb on - strand, within RR42_RS04295 at 954.763 kb on + strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.812 kb on - strand, within RR42_RS04295 at 954.851 kb on - strand, within RR42_RS04295 at 954.851 kb on - strand, within RR42_RS04295 at 954.995 kb on - strand, within RR42_RS04295 at 955.385 kb on - strand, within RR42_RS04295 at 955.406 kb on - strand, within RR42_RS04295 at 955.406 kb on - strand, within RR42_RS04295 at 955.435 kb on + strand, within RR42_RS04295 at 955.435 kb on + strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.525 kb on + strand, within RR42_RS04295 at 955.688 kb on - strand at 955.863 kb on + strand at 955.881 kb on + strand at 955.882 kb on - strand at 955.899 kb on + strand at 956.003 kb on + strand at 956.027 kb on + strand at 956.027 kb on + strand at 956.027 kb on + strand at 956.027 kb on + strand at 956.028 kb on - strand at 956.029 kb on + strand at 956.029 kb on + strand at 956.029 kb on + strand at 956.029 kb on + strand at 956.029 kb on + strand at 956.029 kb on + strand at 956.030 kb on - strand at 956.030 kb on - strand at 956.030 kb on - strand at 956.030 kb on - strand at 956.030 kb on - strand at 956.031 kb on + strand at 956.031 kb on + strand at 956.031 kb on + strand at 956.032 kb on - strand at 956.032 kb on - strand at 956.193 kb on + strand, within RR42_RS04300 at 956.193 kb on + strand, within RR42_RS04300 at 956.194 kb on - strand, within RR42_RS04300 at 956.194 kb on - strand, within RR42_RS04300 at 956.590 kb on + strand, within RR42_RS04305 at 956.591 kb on - strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.592 kb on + strand, within RR42_RS04305 at 956.593 kb on - strand, within RR42_RS04305 at 956.593 kb on - strand, within RR42_RS04305 at 956.625 kb on + strand, within RR42_RS04305 at 956.626 kb on - strand, within RR42_RS04305 at 956.626 kb on - strand, within RR42_RS04305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 953,764 + +1.2 953,764 + +1.0 953,765 - -0.5 953,886 + +1.4 953,900 + +0.2 953,939 + +1.8 953,939 + +1.6 953,955 + +0.7 953,955 + +0.3 953,955 + -0.2 953,986 + +0.3 954,020 + RR42_RS04290 0.13 +0.1 954,020 + RR42_RS04290 0.13 +1.8 954,021 - RR42_RS04290 0.13 +0.4 954,021 - RR42_RS04290 0.13 -0.7 954,043 + RR42_RS04290 0.17 +0.7 954,242 + RR42_RS04290 0.53 -0.9 954,242 + RR42_RS04290 0.53 +1.5 954,242 + RR42_RS04290 0.53 +2.1 954,243 - RR42_RS04290 0.53 +0.1 954,247 + RR42_RS04290 0.54 -0.6 954,247 + RR42_RS04290 0.54 -1.1 954,248 - RR42_RS04290 0.54 +0.2 954,257 + RR42_RS04290 0.56 +0.7 954,415 + RR42_RS04290 0.84 -0.1 954,415 + RR42_RS04290 0.84 -0.3 954,415 + RR42_RS04290 0.84 -1.3 954,415 + RR42_RS04290 0.84 -0.8 954,415 + RR42_RS04290 0.84 +1.4 954,415 + RR42_RS04290 0.84 +1.0 954,415 + RR42_RS04290 0.84 +1.3 954,416 - RR42_RS04290 0.85 -0.0 954,460 + +0.7 954,460 + +1.2 954,460 + +0.8 954,461 - -1.6 954,461 - -0.3 954,461 - +0.1 954,475 - -1.2 954,499 + -0.1 954,574 + +0.7 954,723 - RR42_RS04295 0.15 +0.5 954,760 + RR42_RS04295 0.18 +0.8 954,761 - RR42_RS04295 0.18 -2.4 954,763 + RR42_RS04295 0.18 -0.1 954,764 - RR42_RS04295 0.18 +0.2 954,764 - RR42_RS04295 0.18 -1.0 954,764 - RR42_RS04295 0.18 -0.1 954,764 - RR42_RS04295 0.18 +0.1 954,764 - RR42_RS04295 0.18 +0.4 954,812 - RR42_RS04295 0.22 -0.5 954,851 - RR42_RS04295 0.25 +0.7 954,851 - RR42_RS04295 0.25 +0.5 954,995 - RR42_RS04295 0.37 -0.7 955,385 - RR42_RS04295 0.68 -0.1 955,406 - RR42_RS04295 0.69 -0.1 955,406 - RR42_RS04295 0.69 +0.3 955,435 + RR42_RS04295 0.72 -0.6 955,435 + RR42_RS04295 0.72 +0.5 955,436 - RR42_RS04295 0.72 +1.2 955,436 - RR42_RS04295 0.72 +0.7 955,436 - RR42_RS04295 0.72 +0.6 955,436 - RR42_RS04295 0.72 +0.6 955,525 + RR42_RS04295 0.79 +3.0 955,688 - +1.1 955,863 + +1.7 955,881 + +0.5 955,882 - +1.7 955,899 + -0.3 956,003 + -2.7 956,027 + -0.3 956,027 + +0.1 956,027 + +2.1 956,027 + +0.2 956,028 - +0.8 956,029 + +0.0 956,029 + +1.3 956,029 + +0.5 956,029 + +0.5 956,029 + -0.8 956,029 + -0.7 956,030 - -0.2 956,030 - -1.0 956,030 - +0.4 956,030 - -0.2 956,030 - +0.3 956,031 + -2.2 956,031 + +0.3 956,031 + -0.1 956,032 - +0.2 956,032 - +2.2 956,193 + RR42_RS04300 0.44 -0.3 956,193 + RR42_RS04300 0.44 +0.5 956,194 - RR42_RS04300 0.45 +0.6 956,194 - RR42_RS04300 0.45 -0.7 956,590 + RR42_RS04305 0.18 -0.7 956,591 - RR42_RS04305 0.18 -1.1 956,592 + RR42_RS04305 0.18 -0.1 956,592 + RR42_RS04305 0.18 -0.8 956,592 + RR42_RS04305 0.18 -0.5 956,592 + RR42_RS04305 0.18 -1.2 956,592 + RR42_RS04305 0.18 -0.1 956,593 - RR42_RS04305 0.18 +0.8 956,593 - RR42_RS04305 0.18 +0.3 956,625 + RR42_RS04305 0.23 +0.4 956,626 - RR42_RS04305 0.23 -0.2 956,626 - RR42_RS04305 0.23 +0.6
Or see this region's nucleotide sequence