Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04235 and RR42_RS04240 are separated by 215 nucleotides RR42_RS04240 and RR42_RS04245 are separated by 446 nucleotides RR42_RS04245 and RR42_RS04250 are separated by 26 nucleotides
RR42_RS04235: RR42_RS04235 - glutamate-1-semialdehyde aminotransferase, at 937,426 to 938,715
_RS04235
RR42_RS04240: RR42_RS04240 - rubredoxin, at 938,931 to 939,101
_RS04240
RR42_RS04245: RR42_RS04245 - chemotaxis protein CheY, at 939,548 to 940,042
_RS04245
RR42_RS04250: RR42_RS04250 - chemotaxis protein CheY, at 940,069 to 940,437
_RS04250
Position (kb) 938
939
940 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 938.769 kb on - strand at 939.438 kb on - strand at 939.564 kb on + strand at 939.565 kb on - strand at 939.565 kb on - strand at 939.565 kb on - strand at 939.565 kb on - strand at 939.575 kb on - strand at 939.964 kb on + strand, within RR42_RS04245 at 939.965 kb on - strand, within RR42_RS04245 at 939.965 kb on - strand, within RR42_RS04245 at 939.991 kb on - strand, within RR42_RS04245 at 940.074 kb on + strand at 940.075 kb on - strand at 940.075 kb on - strand at 940.075 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 938,769 - +0.7 939,438 - -0.3 939,564 + -0.6 939,565 - -0.6 939,565 - +2.1 939,565 - +0.4 939,565 - +0.2 939,575 - -0.1 939,964 + RR42_RS04245 0.84 -0.0 939,965 - RR42_RS04245 0.84 +0.4 939,965 - RR42_RS04245 0.84 -2.5 939,991 - RR42_RS04245 0.89 +0.4 940,074 + +3.1 940,075 - +1.3 940,075 - -0.3 940,075 - -0.1
Or see this region's nucleotide sequence