Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03960 and RR42_RS03965 are separated by 186 nucleotides RR42_RS03965 and RR42_RS03970 are separated by 699 nucleotides
RR42_RS03960: RR42_RS03960 - ornithine cyclodeaminase, at 873,560 to 874,636
_RS03960
RR42_RS03965: RR42_RS03965 - DNA-binding protein, at 874,823 to 875,425
_RS03965
RR42_RS03970: RR42_RS03970 - glycosyl transferase family 2, at 876,125 to 876,940
_RS03970
Position (kb)
874
875
876 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 874.053 kb on - strand, within RR42_RS03960 at 874.237 kb on + strand, within RR42_RS03960 at 874.237 kb on + strand, within RR42_RS03960 at 874.238 kb on - strand, within RR42_RS03960 at 874.571 kb on + strand at 874.571 kb on + strand at 874.571 kb on + strand at 874.706 kb on - strand at 874.773 kb on + strand at 874.773 kb on + strand at 874.774 kb on - strand at 874.774 kb on - strand at 874.774 kb on - strand at 874.948 kb on - strand, within RR42_RS03965 at 874.948 kb on - strand, within RR42_RS03965 at 875.052 kb on + strand, within RR42_RS03965 at 875.052 kb on + strand, within RR42_RS03965 at 875.053 kb on - strand, within RR42_RS03965 at 875.055 kb on + strand, within RR42_RS03965 at 875.056 kb on - strand, within RR42_RS03965 at 875.056 kb on - strand, within RR42_RS03965 at 875.089 kb on + strand, within RR42_RS03965 at 875.089 kb on + strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.090 kb on - strand, within RR42_RS03965 at 875.099 kb on - strand, within RR42_RS03965 at 875.272 kb on + strand, within RR42_RS03965 at 875.272 kb on + strand, within RR42_RS03965 at 875.272 kb on + strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.273 kb on - strand, within RR42_RS03965 at 875.276 kb on + strand, within RR42_RS03965 at 875.276 kb on + strand, within RR42_RS03965 at 875.276 kb on + strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.277 kb on - strand, within RR42_RS03965 at 875.339 kb on + strand, within RR42_RS03965 at 875.340 kb on - strand, within RR42_RS03965 at 875.388 kb on + strand at 875.388 kb on + strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.389 kb on - strand at 875.392 kb on - strand at 875.442 kb on + strand at 875.443 kb on - strand at 875.521 kb on + strand at 875.521 kb on + strand at 875.559 kb on - strand at 875.559 kb on - strand at 875.559 kb on - strand at 875.637 kb on + strand at 875.637 kb on + strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.638 kb on - strand at 875.689 kb on - strand at 875.689 kb on - strand at 875.704 kb on + strand at 875.705 kb on - strand at 875.705 kb on - strand at 875.774 kb on + strand at 875.775 kb on - strand at 875.775 kb on - strand at 875.828 kb on + strand at 875.829 kb on - strand at 875.829 kb on - strand at 875.871 kb on + strand at 875.872 kb on - strand at 875.872 kb on - strand at 875.904 kb on - strand at 875.904 kb on - strand at 875.904 kb on - strand at 875.916 kb on + strand at 875.916 kb on + strand at 875.916 kb on + strand at 875.917 kb on - strand at 875.917 kb on - strand at 876.157 kb on - strand at 876.184 kb on - strand at 876.229 kb on - strand, within RR42_RS03970 at 876.229 kb on - strand, within RR42_RS03970 at 876.393 kb on + strand, within RR42_RS03970 at 876.393 kb on + strand, within RR42_RS03970 at 876.394 kb on - strand, within RR42_RS03970 at 876.394 kb on - strand, within RR42_RS03970 at 876.394 kb on - strand, within RR42_RS03970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 874,053 - RR42_RS03960 0.46 -0.3 874,237 + RR42_RS03960 0.63 -1.5 874,237 + RR42_RS03960 0.63 +1.0 874,238 - RR42_RS03960 0.63 -0.3 874,571 + +0.6 874,571 + +2.2 874,571 + +1.1 874,706 - +2.0 874,773 + -1.2 874,773 + -1.4 874,774 - -1.6 874,774 - -1.6 874,774 - +0.1 874,948 - RR42_RS03965 0.21 -3.3 874,948 - RR42_RS03965 0.21 -0.5 875,052 + RR42_RS03965 0.38 -1.0 875,052 + RR42_RS03965 0.38 -2.2 875,053 - RR42_RS03965 0.38 -1.0 875,055 + RR42_RS03965 0.38 -0.8 875,056 - RR42_RS03965 0.39 -1.1 875,056 - RR42_RS03965 0.39 -0.8 875,089 + RR42_RS03965 0.44 -0.9 875,089 + RR42_RS03965 0.44 -1.3 875,090 - RR42_RS03965 0.44 -0.6 875,090 - RR42_RS03965 0.44 -1.0 875,090 - RR42_RS03965 0.44 +0.8 875,090 - RR42_RS03965 0.44 -0.4 875,099 - RR42_RS03965 0.46 -1.2 875,272 + RR42_RS03965 0.74 -1.3 875,272 + RR42_RS03965 0.74 -1.1 875,272 + RR42_RS03965 0.74 -1.8 875,273 - RR42_RS03965 0.75 -1.9 875,273 - RR42_RS03965 0.75 -0.7 875,273 - RR42_RS03965 0.75 -1.0 875,273 - RR42_RS03965 0.75 -0.3 875,273 - RR42_RS03965 0.75 -0.5 875,276 + RR42_RS03965 0.75 -0.6 875,276 + RR42_RS03965 0.75 -0.9 875,276 + RR42_RS03965 0.75 +0.0 875,277 - RR42_RS03965 0.75 -0.9 875,277 - RR42_RS03965 0.75 -1.6 875,277 - RR42_RS03965 0.75 -0.6 875,277 - RR42_RS03965 0.75 -0.8 875,277 - RR42_RS03965 0.75 +0.6 875,277 - RR42_RS03965 0.75 -1.4 875,277 - RR42_RS03965 0.75 -0.2 875,277 - RR42_RS03965 0.75 -2.0 875,339 + RR42_RS03965 0.86 +0.1 875,340 - RR42_RS03965 0.86 -1.4 875,388 + +1.3 875,388 + +0.9 875,389 - -0.4 875,389 - -3.3 875,389 - -0.8 875,389 - -1.4 875,389 - -2.2 875,392 - -0.5 875,442 + -0.4 875,443 - +1.6 875,521 + -1.5 875,521 + -1.0 875,559 - +1.6 875,559 - +2.1 875,559 - +1.9 875,637 + -1.8 875,637 + -0.7 875,638 - +2.1 875,638 - +2.5 875,638 - +2.2 875,638 - +1.7 875,638 - +1.3 875,638 - +1.7 875,638 - +1.1 875,638 - +0.9 875,689 - +2.2 875,689 - +4.4 875,704 + -1.2 875,705 - +1.4 875,705 - +2.3 875,774 + -0.7 875,775 - +0.8 875,775 - +2.2 875,828 + -1.0 875,829 - +2.0 875,829 - +4.3 875,871 + -1.3 875,872 - +1.9 875,872 - +1.7 875,904 - +2.1 875,904 - +2.1 875,904 - +3.1 875,916 + +0.1 875,916 + -0.4 875,916 + -1.1 875,917 - -0.1 875,917 - +1.4 876,157 - +2.1 876,184 - +1.7 876,229 - RR42_RS03970 0.13 +1.7 876,229 - RR42_RS03970 0.13 +1.5 876,393 + RR42_RS03970 0.33 -0.4 876,393 + RR42_RS03970 0.33 -0.3 876,394 - RR42_RS03970 0.33 -0.3 876,394 - RR42_RS03970 0.33 +1.7 876,394 - RR42_RS03970 0.33 +1.4
Or see this region's nucleotide sequence