Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03420 and RR42_RS03425 are separated by 95 nucleotides RR42_RS03425 and RR42_RS03430 are separated by 104 nucleotides
RR42_RS03420: RR42_RS03420 - bile acid:sodium symporter, at 760,908 to 761,930
_RS03420
RR42_RS03425: RR42_RS03425 - LysR family transcriptional regulator, at 762,026 to 762,934
_RS03425
RR42_RS03430: RR42_RS03430 - SAM-dependent methyltransferase, at 763,039 to 763,797
_RS03430
Position (kb)
762
763 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 761.227 kb on + strand, within RR42_RS03420 at 761.227 kb on + strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.228 kb on - strand, within RR42_RS03420 at 761.272 kb on + strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.273 kb on - strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.426 kb on + strand, within RR42_RS03420 at 761.427 kb on - strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.590 kb on + strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.591 kb on - strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.674 kb on + strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.675 kb on - strand, within RR42_RS03420 at 761.874 kb on + strand at 761.874 kb on + strand at 761.874 kb on + strand at 761.874 kb on + strand at 761.875 kb on - strand at 761.946 kb on + strand at 761.963 kb on - strand at 762.033 kb on - strand at 762.296 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.356 kb on + strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.357 kb on - strand, within RR42_RS03425 at 762.465 kb on + strand, within RR42_RS03425 at 762.465 kb on + strand, within RR42_RS03425 at 762.466 kb on - strand, within RR42_RS03425 at 762.597 kb on + strand, within RR42_RS03425 at 762.597 kb on + strand, within RR42_RS03425 at 762.598 kb on - strand, within RR42_RS03425 at 762.891 kb on + strand at 762.891 kb on + strand at 762.891 kb on + strand at 762.892 kb on - strand at 762.952 kb on + strand at 762.968 kb on + strand at 762.976 kb on + strand at 763.017 kb on - strand at 763.024 kb on + strand at 763.040 kb on + strand at 763.103 kb on + strand at 763.114 kb on + strand at 763.114 kb on + strand at 763.283 kb on + strand, within RR42_RS03430 at 763.293 kb on + strand, within RR42_RS03430 at 763.406 kb on + strand, within RR42_RS03430 at 763.592 kb on + strand, within RR42_RS03430 at 763.593 kb on - strand, within RR42_RS03430 at 763.593 kb on - strand, within RR42_RS03430 at 763.745 kb on + strand at 763.893 kb on - strand at 763.897 kb on + strand at 763.897 kb on + strand at 763.897 kb on + strand at 763.897 kb on + strand at 763.898 kb on - strand at 763.898 kb on - strand at 763.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 761,227 + RR42_RS03420 0.31 -0.6 761,227 + RR42_RS03420 0.31 -0.1 761,228 - RR42_RS03420 0.31 -1.0 761,228 - RR42_RS03420 0.31 +1.2 761,228 - RR42_RS03420 0.31 +2.3 761,272 + RR42_RS03420 0.36 +0.5 761,273 - RR42_RS03420 0.36 +0.6 761,273 - RR42_RS03420 0.36 -0.0 761,426 + RR42_RS03420 0.51 +0.4 761,426 + RR42_RS03420 0.51 -0.0 761,427 - RR42_RS03420 0.51 -0.7 761,590 + RR42_RS03420 0.67 -0.3 761,590 + RR42_RS03420 0.67 +0.6 761,590 + RR42_RS03420 0.67 -0.6 761,591 - RR42_RS03420 0.67 -1.3 761,591 - RR42_RS03420 0.67 -0.0 761,591 - RR42_RS03420 0.67 +1.1 761,591 - RR42_RS03420 0.67 -0.1 761,591 - RR42_RS03420 0.67 +1.3 761,674 + RR42_RS03420 0.75 +0.3 761,674 + RR42_RS03420 0.75 +0.1 761,674 + RR42_RS03420 0.75 +0.1 761,674 + RR42_RS03420 0.75 -1.0 761,674 + RR42_RS03420 0.75 -0.5 761,675 - RR42_RS03420 0.75 -0.5 761,675 - RR42_RS03420 0.75 +2.0 761,675 - RR42_RS03420 0.75 +2.6 761,675 - RR42_RS03420 0.75 +0.7 761,874 + +0.5 761,874 + -0.6 761,874 + -0.1 761,874 + +0.3 761,875 - -1.8 761,946 + +1.3 761,963 - +0.9 762,033 - -0.4 762,296 + RR42_RS03425 0.30 +2.9 762,356 + RR42_RS03425 0.36 +0.1 762,356 + RR42_RS03425 0.36 -1.7 762,356 + RR42_RS03425 0.36 +0.4 762,356 + RR42_RS03425 0.36 -0.6 762,356 + RR42_RS03425 0.36 -1.8 762,356 + RR42_RS03425 0.36 +0.2 762,357 - RR42_RS03425 0.36 +0.3 762,357 - RR42_RS03425 0.36 +0.8 762,357 - RR42_RS03425 0.36 -1.6 762,357 - RR42_RS03425 0.36 -0.1 762,357 - RR42_RS03425 0.36 +0.3 762,357 - RR42_RS03425 0.36 -1.4 762,357 - RR42_RS03425 0.36 -0.1 762,357 - RR42_RS03425 0.36 +0.3 762,357 - RR42_RS03425 0.36 +0.3 762,465 + RR42_RS03425 0.48 +1.0 762,465 + RR42_RS03425 0.48 +0.1 762,466 - RR42_RS03425 0.48 -0.2 762,597 + RR42_RS03425 0.63 +0.3 762,597 + RR42_RS03425 0.63 +1.0 762,598 - RR42_RS03425 0.63 -0.2 762,891 + +0.6 762,891 + +0.7 762,891 + -0.0 762,892 - +0.5 762,952 + +1.9 762,968 + +0.7 762,976 + +0.2 763,017 - -0.3 763,024 + -0.1 763,040 + -0.3 763,103 + -0.7 763,114 + +1.3 763,114 + -1.9 763,283 + RR42_RS03430 0.32 -0.1 763,293 + RR42_RS03430 0.33 +0.0 763,406 + RR42_RS03430 0.48 +0.8 763,592 + RR42_RS03430 0.73 +1.3 763,593 - RR42_RS03430 0.73 -0.5 763,593 - RR42_RS03430 0.73 -0.7 763,745 + +0.1 763,893 - +0.5 763,897 + +0.5 763,897 + -0.9 763,897 + -0.3 763,897 + -0.4 763,898 - +0.4 763,898 - +1.9 763,898 - +2.3
Or see this region's nucleotide sequence