Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03245 and RR42_RS03250 are separated by 138 nucleotides RR42_RS03250 and RR42_RS03255 are separated by 39 nucleotides RR42_RS03255 and RR42_RS03260 overlap by 4 nucleotides
RR42_RS03245: RR42_RS03245 - D-alanyl-D-alanine carboxypeptidase, at 719,185 to 720,717
_RS03245
RR42_RS03250: RR42_RS03250 - MarR family transcriptional regulator, at 720,856 to 721,416
_RS03250
RR42_RS03255: RR42_RS03255 - hypothetical protein, at 721,456 to 721,956
_RS03255
RR42_RS03260: RR42_RS03260 - branched-chain amino acid ABC transporter substrate-binding protein, at 721,953 to 723,071
_RS03260
Position (kb) 720
721
722 Strain fitness (log2 ratio) -2
-1
0
1
2 at 719.888 kb on + strand, within RR42_RS03245 at 719.888 kb on + strand, within RR42_RS03245 at 720.073 kb on - strand, within RR42_RS03245 at 720.095 kb on + strand, within RR42_RS03245 at 720.096 kb on - strand, within RR42_RS03245 at 720.122 kb on + strand, within RR42_RS03245 at 720.123 kb on - strand, within RR42_RS03245 at 720.179 kb on + strand, within RR42_RS03245 at 720.179 kb on + strand, within RR42_RS03245 at 720.296 kb on + strand, within RR42_RS03245 at 720.296 kb on + strand, within RR42_RS03245 at 720.344 kb on + strand, within RR42_RS03245 at 720.345 kb on - strand, within RR42_RS03245 at 720.488 kb on + strand, within RR42_RS03245 at 720.489 kb on - strand, within RR42_RS03245 at 720.721 kb on - strand at 721.007 kb on + strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.221 kb on + strand, within RR42_RS03250 at 721.457 kb on + strand at 721.458 kb on - strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.460 kb on - strand at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.671 kb on - strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 722.038 kb on + strand at 722.039 kb on - strand at 722.067 kb on - strand, within RR42_RS03260 at 722.067 kb on - strand, within RR42_RS03260 at 722.180 kb on - strand, within RR42_RS03260 at 722.180 kb on - strand, within RR42_RS03260 at 722.219 kb on - strand, within RR42_RS03260 at 722.242 kb on + strand, within RR42_RS03260 at 722.384 kb on - strand, within RR42_RS03260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 719,888 + RR42_RS03245 0.46 +1.1 719,888 + RR42_RS03245 0.46 +0.9 720,073 - RR42_RS03245 0.58 +1.1 720,095 + RR42_RS03245 0.59 +1.1 720,096 - RR42_RS03245 0.59 +0.0 720,122 + RR42_RS03245 0.61 +1.9 720,123 - RR42_RS03245 0.61 +1.4 720,179 + RR42_RS03245 0.65 +1.9 720,179 + RR42_RS03245 0.65 +0.4 720,296 + RR42_RS03245 0.72 +0.8 720,296 + RR42_RS03245 0.72 -0.7 720,344 + RR42_RS03245 0.76 +1.1 720,345 - RR42_RS03245 0.76 +1.4 720,488 + RR42_RS03245 0.85 +0.6 720,489 - RR42_RS03245 0.85 +2.5 720,721 - -0.6 721,007 + RR42_RS03250 0.27 -0.3 721,218 - RR42_RS03250 0.65 +0.7 721,218 - RR42_RS03250 0.65 -1.5 721,221 + RR42_RS03250 0.65 -1.4 721,457 + -1.5 721,458 - +0.0 721,459 + -1.6 721,459 + -0.9 721,459 + +0.4 721,459 + -0.3 721,459 + -1.3 721,460 - -0.5 721,583 + RR42_RS03255 0.25 -0.1 721,583 + RR42_RS03255 0.25 -1.3 721,583 + RR42_RS03255 0.25 -0.3 721,670 + RR42_RS03255 0.43 +0.2 721,670 + RR42_RS03255 0.43 -0.5 721,670 + RR42_RS03255 0.43 -1.6 721,670 + RR42_RS03255 0.43 -1.5 721,671 - RR42_RS03255 0.43 +0.7 721,703 + RR42_RS03255 0.49 +0.1 721,703 + RR42_RS03255 0.49 +0.4 722,038 + -0.9 722,039 - +0.1 722,067 - RR42_RS03260 0.10 -0.8 722,067 - RR42_RS03260 0.10 -0.5 722,180 - RR42_RS03260 0.20 -0.1 722,180 - RR42_RS03260 0.20 -2.1 722,219 - RR42_RS03260 0.24 +0.2 722,242 + RR42_RS03260 0.26 +0.3 722,384 - RR42_RS03260 0.39 -0.0
Or see this region's nucleotide sequence