Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03240 and RR42_RS03245 are separated by 384 nucleotides RR42_RS03245 and RR42_RS03250 are separated by 138 nucleotides RR42_RS03250 and RR42_RS03255 are separated by 39 nucleotides
RR42_RS03240: RR42_RS03240 - integrase, at 717,628 to 718,800
_RS03240
RR42_RS03245: RR42_RS03245 - D-alanyl-D-alanine carboxypeptidase, at 719,185 to 720,717
_RS03245
RR42_RS03250: RR42_RS03250 - MarR family transcriptional regulator, at 720,856 to 721,416
_RS03250
RR42_RS03255: RR42_RS03255 - hypothetical protein, at 721,456 to 721,956
_RS03255
Position (kb) 719
720
721 Strain fitness (log2 ratio) -1
0
1
2 at 718.815 kb on + strand at 718.834 kb on + strand at 718.977 kb on + strand at 719.264 kb on + strand at 719.264 kb on + strand at 719.265 kb on - strand at 719.265 kb on - strand at 719.280 kb on - strand at 719.306 kb on + strand at 719.307 kb on - strand at 719.351 kb on + strand, within RR42_RS03245 at 719.351 kb on + strand, within RR42_RS03245 at 719.351 kb on + strand, within RR42_RS03245 at 719.351 kb on + strand, within RR42_RS03245 at 719.352 kb on - strand, within RR42_RS03245 at 719.352 kb on - strand, within RR42_RS03245 at 719.481 kb on + strand, within RR42_RS03245 at 719.481 kb on + strand, within RR42_RS03245 at 719.482 kb on - strand, within RR42_RS03245 at 719.482 kb on - strand, within RR42_RS03245 at 719.482 kb on - strand, within RR42_RS03245 at 719.487 kb on - strand, within RR42_RS03245 at 719.487 kb on - strand, within RR42_RS03245 at 719.487 kb on - strand, within RR42_RS03245 at 719.888 kb on + strand, within RR42_RS03245 at 719.888 kb on + strand, within RR42_RS03245 at 720.073 kb on - strand, within RR42_RS03245 at 720.095 kb on + strand, within RR42_RS03245 at 720.096 kb on - strand, within RR42_RS03245 at 720.122 kb on + strand, within RR42_RS03245 at 720.123 kb on - strand, within RR42_RS03245 at 720.179 kb on + strand, within RR42_RS03245 at 720.179 kb on + strand, within RR42_RS03245 at 720.296 kb on + strand, within RR42_RS03245 at 720.296 kb on + strand, within RR42_RS03245 at 720.344 kb on + strand, within RR42_RS03245 at 720.345 kb on - strand, within RR42_RS03245 at 720.488 kb on + strand, within RR42_RS03245 at 720.489 kb on - strand, within RR42_RS03245 at 720.721 kb on - strand at 721.007 kb on + strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.221 kb on + strand, within RR42_RS03250 at 721.457 kb on + strand at 721.458 kb on - strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.460 kb on - strand at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.671 kb on - strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 718,815 + -1.7 718,834 + +0.4 718,977 + -0.6 719,264 + +1.6 719,264 + +1.3 719,265 - +0.9 719,265 - +2.1 719,280 - +2.1 719,306 + +0.2 719,307 - +1.2 719,351 + RR42_RS03245 0.11 +0.8 719,351 + RR42_RS03245 0.11 +0.7 719,351 + RR42_RS03245 0.11 +0.5 719,351 + RR42_RS03245 0.11 +1.0 719,352 - RR42_RS03245 0.11 +0.9 719,352 - RR42_RS03245 0.11 +2.2 719,481 + RR42_RS03245 0.19 +1.3 719,481 + RR42_RS03245 0.19 +1.8 719,482 - RR42_RS03245 0.19 +1.6 719,482 - RR42_RS03245 0.19 +1.3 719,482 - RR42_RS03245 0.19 +1.0 719,487 - RR42_RS03245 0.20 +1.8 719,487 - RR42_RS03245 0.20 +0.9 719,487 - RR42_RS03245 0.20 +1.4 719,888 + RR42_RS03245 0.46 +1.1 719,888 + RR42_RS03245 0.46 +0.9 720,073 - RR42_RS03245 0.58 +1.1 720,095 + RR42_RS03245 0.59 +1.1 720,096 - RR42_RS03245 0.59 +0.0 720,122 + RR42_RS03245 0.61 +1.9 720,123 - RR42_RS03245 0.61 +1.4 720,179 + RR42_RS03245 0.65 +1.9 720,179 + RR42_RS03245 0.65 +0.4 720,296 + RR42_RS03245 0.72 +0.8 720,296 + RR42_RS03245 0.72 -0.7 720,344 + RR42_RS03245 0.76 +1.1 720,345 - RR42_RS03245 0.76 +1.4 720,488 + RR42_RS03245 0.85 +0.6 720,489 - RR42_RS03245 0.85 +2.5 720,721 - -0.6 721,007 + RR42_RS03250 0.27 -0.3 721,218 - RR42_RS03250 0.65 +0.7 721,218 - RR42_RS03250 0.65 -1.5 721,221 + RR42_RS03250 0.65 -1.4 721,457 + -1.5 721,458 - +0.0 721,459 + -1.6 721,459 + -0.9 721,459 + +0.4 721,459 + -0.3 721,459 + -1.3 721,460 - -0.5 721,583 + RR42_RS03255 0.25 -0.1 721,583 + RR42_RS03255 0.25 -1.3 721,583 + RR42_RS03255 0.25 -0.3 721,670 + RR42_RS03255 0.43 +0.2 721,670 + RR42_RS03255 0.43 -0.5 721,670 + RR42_RS03255 0.43 -1.6 721,670 + RR42_RS03255 0.43 -1.5 721,671 - RR42_RS03255 0.43 +0.7 721,703 + RR42_RS03255 0.49 +0.1 721,703 + RR42_RS03255 0.49 +0.4
Or see this region's nucleotide sequence