Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03220 and RR42_RS03225 are separated by 61 nucleotides RR42_RS03225 and RR42_RS03230 are separated by 59 nucleotides RR42_RS03230 and RR42_RS03235 are separated by 105 nucleotides
RR42_RS03220: RR42_RS03220 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 713,497 to 714,405
_RS03220
RR42_RS03225: RR42_RS03225 - N5-carboxyaminoimidazole ribonucleotide mutase, at 714,467 to 714,964
_RS03225
RR42_RS03230: RR42_RS03230 - phosphoribosylaminoimidazole carboxylase, at 715,024 to 716,268
_RS03230
RR42_RS03235: RR42_RS03235 - translation factor Sua5, at 716,374 to 717,360
_RS03235
Position (kb)
715
716
717 Strain fitness (log2 ratio)
-2
-1
0
1 at 714.451 kb on + strand at 714.451 kb on + strand at 714.455 kb on + strand at 714.962 kb on + strand at 714.965 kb on + strand at 714.965 kb on + strand at 714.965 kb on + strand at 714.965 kb on + strand at 714.965 kb on + strand at 714.965 kb on + strand at 714.983 kb on + strand at 716.324 kb on + strand at 716.324 kb on + strand at 716.324 kb on + strand at 716.324 kb on + strand at 716.324 kb on + strand at 716.324 kb on + strand at 716.332 kb on + strand at 716.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 714,451 + +0.1 714,451 + +0.7 714,455 + -0.5 714,962 + -0.7 714,965 + -1.9 714,965 + +0.1 714,965 + +0.3 714,965 + +0.0 714,965 + -0.9 714,965 + +0.6 714,983 + +0.1 716,324 + -1.8 716,324 + +0.5 716,324 + +0.7 716,324 + +0.6 716,324 + -0.1 716,324 + -0.2 716,332 + -1.2 716,348 + +0.1
Or see this region's nucleotide sequence