Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS03230

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS03220 and RR42_RS03225 are separated by 61 nucleotidesRR42_RS03225 and RR42_RS03230 are separated by 59 nucleotidesRR42_RS03230 and RR42_RS03235 are separated by 105 nucleotides RR42_RS03220: RR42_RS03220 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 713,497 to 714,405 _RS03220 RR42_RS03225: RR42_RS03225 - N5-carboxyaminoimidazole ribonucleotide mutase, at 714,467 to 714,964 _RS03225 RR42_RS03230: RR42_RS03230 - phosphoribosylaminoimidazole carboxylase, at 715,024 to 716,268 _RS03230 RR42_RS03235: RR42_RS03235 - translation factor Sua5, at 716,374 to 717,360 _RS03235 Position (kb) 715 716 717Strain fitness (log2 ratio) -2 -1 0 1at 714.451 kb on + strandat 714.451 kb on + strandat 714.455 kb on + strandat 714.962 kb on + strandat 714.965 kb on + strandat 714.965 kb on + strandat 714.965 kb on + strandat 714.965 kb on + strandat 714.965 kb on + strandat 714.965 kb on + strandat 714.983 kb on + strandat 716.324 kb on + strandat 716.324 kb on + strandat 716.324 kb on + strandat 716.324 kb on + strandat 716.324 kb on + strandat 716.324 kb on + strandat 716.332 kb on + strandat 716.348 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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714,451 + +0.1
714,451 + +0.7
714,455 + -0.5
714,962 + -0.7
714,965 + -1.9
714,965 + +0.1
714,965 + +0.3
714,965 + +0.0
714,965 + -0.9
714,965 + +0.6
714,983 + +0.1
716,324 + -1.8
716,324 + +0.5
716,324 + +0.7
716,324 + +0.6
716,324 + -0.1
716,324 + -0.2
716,332 + -1.2
716,348 + +0.1

Or see this region's nucleotide sequence