Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03145 and RR42_RS03150 are separated by 48 nucleotides RR42_RS03150 and RR42_RS03155 are separated by 77 nucleotides RR42_RS03155 and RR42_RS03160 are separated by 160 nucleotides RR42_RS03160 and RR42_RS03165 are separated by 57 nucleotides
RR42_RS03145: RR42_RS03145 - peptidase M48, at 694,642 to 696,450
_RS03145
RR42_RS03150: RR42_RS03150 - hypothetical protein, at 696,499 to 697,110
_RS03150
RR42_RS03155: RR42_RS03155 - alpha/beta hydrolase, at 697,188 to 698,174
_RS03155
RR42_RS03160: RR42_RS03160 - hypothetical protein, at 698,335 to 698,775
_RS03160
RR42_RS03165: RR42_RS03165 - ADP-heptose--LPS heptosyltransferase, at 698,833 to 699,855
_RS03165
Position (kb)
697
698
699 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 696.236 kb on - strand, within RR42_RS03145 at 696.236 kb on - strand, within RR42_RS03145 at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.287 kb on - strand at 696.287 kb on - strand at 696.305 kb on - strand at 696.361 kb on + strand at 696.362 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.614 kb on - strand, within RR42_RS03150 at 696.663 kb on + strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.905 kb on + strand, within RR42_RS03150 at 697.004 kb on + strand, within RR42_RS03150 at 697.005 kb on - strand, within RR42_RS03150 at 697.055 kb on + strand at 697.066 kb on + strand at 697.168 kb on - strand at 697.168 kb on - strand at 697.239 kb on - strand at 697.277 kb on + strand at 697.277 kb on + strand at 697.278 kb on - strand at 697.370 kb on - strand, within RR42_RS03155 at 697.509 kb on - strand, within RR42_RS03155 at 697.509 kb on - strand, within RR42_RS03155 at 697.522 kb on + strand, within RR42_RS03155 at 697.627 kb on + strand, within RR42_RS03155 at 697.627 kb on + strand, within RR42_RS03155 at 697.627 kb on + strand, within RR42_RS03155 at 697.628 kb on - strand, within RR42_RS03155 at 697.807 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.864 kb on + strand, within RR42_RS03155 at 697.966 kb on + strand, within RR42_RS03155 at 698.298 kb on + strand at 698.298 kb on + strand at 698.337 kb on - strand at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.447 kb on + strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.448 kb on - strand, within RR42_RS03160 at 698.462 kb on + strand, within RR42_RS03160 at 698.462 kb on + strand, within RR42_RS03160 at 698.462 kb on + strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.463 kb on - strand, within RR42_RS03160 at 698.538 kb on + strand, within RR42_RS03160 at 698.538 kb on + strand, within RR42_RS03160 at 698.538 kb on + strand, within RR42_RS03160 at 698.539 kb on - strand, within RR42_RS03160 at 698.539 kb on - strand, within RR42_RS03160 at 698.539 kb on - strand, within RR42_RS03160 at 698.705 kb on - strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.731 kb on + strand, within RR42_RS03160 at 698.732 kb on - strand at 698.732 kb on - strand at 698.732 kb on - strand at 698.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 696,236 - RR42_RS03145 0.88 -2.5 696,236 - RR42_RS03145 0.88 -1.7 696,286 + -0.2 696,286 + -1.3 696,286 + -0.3 696,286 + -1.3 696,286 + -0.2 696,287 - -1.4 696,287 - -0.5 696,305 - -2.0 696,361 + -0.7 696,362 - -2.1 696,445 - -0.3 696,445 - +0.4 696,445 - +0.6 696,614 - RR42_RS03150 0.19 +0.3 696,663 + RR42_RS03150 0.27 -0.2 696,664 - RR42_RS03150 0.27 +1.1 696,664 - RR42_RS03150 0.27 +0.4 696,864 - RR42_RS03150 0.60 -1.0 696,864 - RR42_RS03150 0.60 -0.5 696,905 + RR42_RS03150 0.66 +0.1 697,004 + RR42_RS03150 0.83 -0.5 697,005 - RR42_RS03150 0.83 +0.2 697,055 + -0.4 697,066 + -0.1 697,168 - +0.5 697,168 - +0.3 697,239 - +0.6 697,277 + +0.9 697,277 + +0.9 697,278 - +1.4 697,370 - RR42_RS03155 0.18 -0.5 697,509 - RR42_RS03155 0.33 -1.0 697,509 - RR42_RS03155 0.33 -0.8 697,522 + RR42_RS03155 0.34 +0.2 697,627 + RR42_RS03155 0.44 -0.8 697,627 + RR42_RS03155 0.44 +0.8 697,627 + RR42_RS03155 0.44 +1.2 697,628 - RR42_RS03155 0.45 +0.6 697,807 + RR42_RS03155 0.63 +0.3 697,864 + RR42_RS03155 0.68 +0.5 697,864 + RR42_RS03155 0.68 -0.4 697,864 + RR42_RS03155 0.68 -0.1 697,864 + RR42_RS03155 0.68 +0.7 697,966 + RR42_RS03155 0.79 +0.1 698,298 + +0.8 698,298 + +0.4 698,337 - -1.1 698,447 + RR42_RS03160 0.25 +1.3 698,447 + RR42_RS03160 0.25 +0.9 698,447 + RR42_RS03160 0.25 -0.2 698,447 + RR42_RS03160 0.25 +0.5 698,447 + RR42_RS03160 0.25 +0.4 698,447 + RR42_RS03160 0.25 -0.5 698,448 - RR42_RS03160 0.26 -0.5 698,448 - RR42_RS03160 0.26 -0.0 698,448 - RR42_RS03160 0.26 +0.6 698,448 - RR42_RS03160 0.26 +0.6 698,462 + RR42_RS03160 0.29 -1.3 698,462 + RR42_RS03160 0.29 +0.1 698,462 + RR42_RS03160 0.29 -0.2 698,463 - RR42_RS03160 0.29 -0.5 698,463 - RR42_RS03160 0.29 -0.6 698,463 - RR42_RS03160 0.29 -0.1 698,463 - RR42_RS03160 0.29 -0.5 698,463 - RR42_RS03160 0.29 +0.0 698,538 + RR42_RS03160 0.46 +1.9 698,538 + RR42_RS03160 0.46 +0.8 698,538 + RR42_RS03160 0.46 +1.8 698,539 - RR42_RS03160 0.46 +0.9 698,539 - RR42_RS03160 0.46 -1.7 698,539 - RR42_RS03160 0.46 +1.2 698,705 - RR42_RS03160 0.84 +1.7 698,731 + RR42_RS03160 0.90 +0.0 698,731 + RR42_RS03160 0.90 +1.0 698,731 + RR42_RS03160 0.90 -0.0 698,731 + RR42_RS03160 0.90 +0.7 698,731 + RR42_RS03160 0.90 -0.9 698,731 + RR42_RS03160 0.90 -0.9 698,731 + RR42_RS03160 0.90 +1.1 698,732 - -0.8 698,732 - +0.6 698,732 - -1.3 698,732 - +0.7
Or see this region's nucleotide sequence