Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03105 and RR42_RS03110 are separated by 380 nucleotides RR42_RS03110 and RR42_RS03115 are separated by 102 nucleotides RR42_RS03115 and RR42_RS03120 are separated by 29 nucleotides RR42_RS03120 and RR42_RS03125 are separated by 163 nucleotides
RR42_RS03105: RR42_RS03105 - integral membrane protein, at 686,610 to 687,305
_RS03105
RR42_RS03110: RR42_RS03110 - pilus assembly protein TapA, at 687,686 to 688,144
_RS03110
RR42_RS03115: RR42_RS03115 - ligase, at 688,247 to 690,127
_RS03115
RR42_RS03120: RR42_RS03120 - proline-rich region, at 690,157 to 690,648
_RS03120
RR42_RS03125: RR42_RS03125 - hypothetical protein, at 690,812 to 691,306
_RS03125
Position (kb)
688
689
690
691 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.315 kb on + strand at 687.316 kb on - strand at 687.316 kb on - strand at 687.392 kb on + strand at 687.393 kb on - strand at 687.393 kb on - strand at 687.461 kb on - strand at 687.461 kb on - strand at 687.465 kb on + strand at 687.484 kb on + strand at 687.484 kb on + strand at 687.484 kb on + strand at 687.485 kb on - strand at 687.485 kb on - strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.488 kb on + strand at 687.489 kb on - strand at 687.489 kb on - strand at 687.489 kb on - strand at 687.588 kb on + strand at 687.595 kb on + strand at 687.595 kb on + strand at 687.596 kb on - strand at 687.623 kb on + strand at 687.623 kb on + strand at 687.797 kb on + strand, within RR42_RS03110 at 687.797 kb on + strand, within RR42_RS03110 at 687.797 kb on + strand, within RR42_RS03110 at 687.798 kb on - strand, within RR42_RS03110 at 687.838 kb on - strand, within RR42_RS03110 at 687.898 kb on + strand, within RR42_RS03110 at 687.899 kb on - strand, within RR42_RS03110 at 687.941 kb on - strand, within RR42_RS03110 at 688.072 kb on + strand, within RR42_RS03110 at 688.072 kb on + strand, within RR42_RS03110 at 688.073 kb on - strand, within RR42_RS03110 at 688.146 kb on + strand at 688.147 kb on - strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.150 kb on + strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.151 kb on - strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.204 kb on + strand at 688.205 kb on - strand at 688.205 kb on - strand at 688.205 kb on - strand at 688.231 kb on + strand at 688.231 kb on + strand at 688.232 kb on - strand at 688.336 kb on - strand at 688.346 kb on + strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.347 kb on - strand at 688.433 kb on + strand at 688.707 kb on + strand, within RR42_RS03115 at 688.755 kb on - strand, within RR42_RS03115 at 688.755 kb on - strand, within RR42_RS03115 at 688.964 kb on + strand, within RR42_RS03115 at 688.964 kb on + strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 688.965 kb on - strand, within RR42_RS03115 at 689.200 kb on + strand, within RR42_RS03115 at 689.312 kb on + strand, within RR42_RS03115 at 689.312 kb on + strand, within RR42_RS03115 at 689.312 kb on + strand, within RR42_RS03115 at 689.313 kb on - strand, within RR42_RS03115 at 689.579 kb on + strand, within RR42_RS03115 at 689.579 kb on + strand, within RR42_RS03115 at 689.580 kb on - strand, within RR42_RS03115 at 689.580 kb on - strand, within RR42_RS03115 at 689.586 kb on - strand, within RR42_RS03115 at 689.732 kb on + strand, within RR42_RS03115 at 689.733 kb on - strand, within RR42_RS03115 at 689.896 kb on - strand, within RR42_RS03115 at 689.896 kb on - strand, within RR42_RS03115 at 689.999 kb on + strand at 689.999 kb on + strand at 689.999 kb on + strand at 690.125 kb on + strand at 690.125 kb on + strand at 690.125 kb on + strand at 690.126 kb on - strand at 690.126 kb on - strand at 690.158 kb on + strand at 690.200 kb on + strand at 690.201 kb on - strand at 690.201 kb on - strand at 690.201 kb on - strand at 690.201 kb on - strand at 690.201 kb on - strand at 690.203 kb on + strand at 690.203 kb on + strand at 690.203 kb on + strand at 690.203 kb on + strand at 690.204 kb on - strand at 690.204 kb on - strand at 690.204 kb on - strand at 690.204 kb on - strand at 690.204 kb on - strand at 690.204 kb on - strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.206 kb on + strand at 690.207 kb on - strand, within RR42_RS03120 at 690.207 kb on - strand, within RR42_RS03120 at 690.207 kb on - strand, within RR42_RS03120 at 690.207 kb on - strand, within RR42_RS03120 at 690.207 kb on - strand, within RR42_RS03120 at 690.278 kb on + strand, within RR42_RS03120 at 690.278 kb on + strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.279 kb on - strand, within RR42_RS03120 at 690.332 kb on + strand, within RR42_RS03120 at 690.333 kb on - strand, within RR42_RS03120 at 690.333 kb on - strand, within RR42_RS03120 at 690.505 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.534 kb on + strand, within RR42_RS03120 at 690.535 kb on - strand, within RR42_RS03120 at 690.535 kb on - strand, within RR42_RS03120 at 690.535 kb on - strand, within RR42_RS03120 at 690.535 kb on - strand, within RR42_RS03120 at 690.536 kb on + strand, within RR42_RS03120 at 690.714 kb on - strand at 690.761 kb on - strand at 690.865 kb on + strand, within RR42_RS03125 at 690.865 kb on + strand, within RR42_RS03125 at 690.865 kb on + strand, within RR42_RS03125 at 690.866 kb on - strand, within RR42_RS03125 at 690.866 kb on - strand, within RR42_RS03125 at 690.866 kb on - strand, within RR42_RS03125 at 690.867 kb on + strand, within RR42_RS03125 at 690.868 kb on - strand, within RR42_RS03125 at 690.868 kb on - strand, within RR42_RS03125 at 690.868 kb on - strand, within RR42_RS03125 at 690.868 kb on - strand, within RR42_RS03125 at 690.868 kb on - strand, within RR42_RS03125 at 690.874 kb on - strand, within RR42_RS03125 at 690.876 kb on + strand, within RR42_RS03125 at 690.876 kb on + strand, within RR42_RS03125 at 690.876 kb on + strand, within RR42_RS03125 at 690.900 kb on + strand, within RR42_RS03125 at 690.901 kb on - strand, within RR42_RS03125 at 690.906 kb on + strand, within RR42_RS03125 at 690.906 kb on + strand, within RR42_RS03125 at 690.907 kb on - strand, within RR42_RS03125 at 690.907 kb on - strand, within RR42_RS03125 at 690.954 kb on + strand, within RR42_RS03125 at 690.984 kb on + strand, within RR42_RS03125 at 690.984 kb on + strand, within RR42_RS03125 at 690.985 kb on - strand, within RR42_RS03125 at 690.985 kb on - strand, within RR42_RS03125 at 691.026 kb on + strand, within RR42_RS03125 at 691.048 kb on - strand, within RR42_RS03125 at 691.048 kb on - strand, within RR42_RS03125 at 691.050 kb on + strand, within RR42_RS03125 at 691.051 kb on - strand, within RR42_RS03125 at 691.101 kb on + strand, within RR42_RS03125 at 691.102 kb on - strand, within RR42_RS03125 at 691.102 kb on - strand, within RR42_RS03125 at 691.102 kb on - strand, within RR42_RS03125 at 691.102 kb on - strand, within RR42_RS03125 at 691.102 kb on - strand, within RR42_RS03125 at 691.102 kb on - strand, within RR42_RS03125 at 691.109 kb on + strand, within RR42_RS03125 at 691.109 kb on + strand, within RR42_RS03125 at 691.110 kb on - strand, within RR42_RS03125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 687,315 + -0.3 687,315 + +0.2 687,315 + -0.3 687,315 + +2.5 687,315 + +1.9 687,315 + +0.9 687,316 - +0.2 687,316 - -0.3 687,392 + +1.2 687,393 - -0.0 687,393 - -0.8 687,461 - +1.2 687,461 - -1.2 687,465 + +1.4 687,484 + +0.8 687,484 + +0.3 687,484 + -0.7 687,485 - -1.8 687,485 - +0.8 687,488 + +0.6 687,488 + -0.2 687,488 + +1.2 687,488 + -1.3 687,488 + +0.8 687,488 + +0.9 687,488 + -0.6 687,488 + +2.5 687,488 + +0.2 687,488 + +0.7 687,489 - -0.3 687,489 - +0.4 687,489 - +1.3 687,588 + -0.7 687,595 + +0.8 687,595 + +0.2 687,596 - -0.4 687,623 + +0.3 687,623 + +0.3 687,797 + RR42_RS03110 0.24 -0.5 687,797 + RR42_RS03110 0.24 -0.4 687,797 + RR42_RS03110 0.24 -0.0 687,798 - RR42_RS03110 0.24 -0.7 687,838 - RR42_RS03110 0.33 -1.0 687,898 + RR42_RS03110 0.46 -0.3 687,899 - RR42_RS03110 0.46 -0.6 687,941 - RR42_RS03110 0.56 +0.3 688,072 + RR42_RS03110 0.84 -0.7 688,072 + RR42_RS03110 0.84 +0.8 688,073 - RR42_RS03110 0.84 -0.5 688,146 + +0.0 688,147 - -0.5 688,150 + -0.5 688,150 + -0.9 688,150 + -1.1 688,150 + -0.5 688,151 - -0.5 688,151 - -0.3 688,151 - -0.5 688,151 - -0.1 688,151 - -0.5 688,151 - -2.0 688,151 - -1.5 688,151 - -0.7 688,204 + -1.6 688,204 + +1.2 688,204 + +0.1 688,204 + -0.3 688,205 - -1.6 688,205 - -0.5 688,205 - -0.2 688,231 + -0.4 688,231 + +0.4 688,232 - +0.8 688,336 - +1.1 688,346 + +1.1 688,347 - -0.8 688,347 - -0.1 688,347 - +1.3 688,347 - +0.9 688,433 + +0.7 688,707 + RR42_RS03115 0.24 +1.5 688,755 - RR42_RS03115 0.27 +1.1 688,755 - RR42_RS03115 0.27 +1.6 688,964 + RR42_RS03115 0.38 +0.7 688,964 + RR42_RS03115 0.38 +2.1 688,965 - RR42_RS03115 0.38 +0.8 688,965 - RR42_RS03115 0.38 +1.0 688,965 - RR42_RS03115 0.38 +1.4 689,200 + RR42_RS03115 0.51 +0.9 689,312 + RR42_RS03115 0.57 +1.8 689,312 + RR42_RS03115 0.57 -0.2 689,312 + RR42_RS03115 0.57 +3.8 689,313 - RR42_RS03115 0.57 +1.2 689,579 + RR42_RS03115 0.71 +0.5 689,579 + RR42_RS03115 0.71 +2.4 689,580 - RR42_RS03115 0.71 +1.6 689,580 - RR42_RS03115 0.71 +1.2 689,586 - RR42_RS03115 0.71 +1.0 689,732 + RR42_RS03115 0.79 +1.3 689,733 - RR42_RS03115 0.79 +1.7 689,896 - RR42_RS03115 0.88 +1.9 689,896 - RR42_RS03115 0.88 +0.9 689,999 + +1.2 689,999 + +1.1 689,999 + +1.3 690,125 + +0.4 690,125 + +0.2 690,125 + -0.0 690,126 - +0.3 690,126 - +0.8 690,158 + -0.4 690,200 + -1.0 690,201 - +1.0 690,201 - -0.3 690,201 - -1.7 690,201 - +0.8 690,201 - +0.5 690,203 + -0.5 690,203 + +0.2 690,203 + +0.2 690,203 + -0.8 690,204 - +0.6 690,204 - -0.7 690,204 - +0.7 690,204 - +0.4 690,204 - +1.3 690,204 - +0.4 690,206 + -1.1 690,206 + -0.3 690,206 + +0.7 690,206 + +0.0 690,206 + +0.2 690,206 + -0.1 690,206 + -1.0 690,206 + +0.3 690,206 + -0.0 690,207 - RR42_RS03120 0.10 +0.1 690,207 - RR42_RS03120 0.10 +0.7 690,207 - RR42_RS03120 0.10 +0.3 690,207 - RR42_RS03120 0.10 +1.3 690,207 - RR42_RS03120 0.10 -0.3 690,278 + RR42_RS03120 0.25 -0.3 690,278 + RR42_RS03120 0.25 +1.3 690,279 - RR42_RS03120 0.25 -0.0 690,279 - RR42_RS03120 0.25 +0.3 690,279 - RR42_RS03120 0.25 +0.4 690,279 - RR42_RS03120 0.25 -0.4 690,279 - RR42_RS03120 0.25 +0.4 690,279 - RR42_RS03120 0.25 +0.2 690,279 - RR42_RS03120 0.25 +0.2 690,279 - RR42_RS03120 0.25 -0.7 690,279 - RR42_RS03120 0.25 -0.4 690,332 + RR42_RS03120 0.36 +1.2 690,333 - RR42_RS03120 0.36 +0.2 690,333 - RR42_RS03120 0.36 +0.6 690,505 + RR42_RS03120 0.71 +2.3 690,534 + RR42_RS03120 0.77 -0.5 690,534 + RR42_RS03120 0.77 -0.2 690,534 + RR42_RS03120 0.77 -0.8 690,534 + RR42_RS03120 0.77 +0.6 690,534 + RR42_RS03120 0.77 -1.4 690,534 + RR42_RS03120 0.77 -1.2 690,534 + RR42_RS03120 0.77 +1.1 690,534 + RR42_RS03120 0.77 -0.3 690,535 - RR42_RS03120 0.77 -0.4 690,535 - RR42_RS03120 0.77 +1.9 690,535 - RR42_RS03120 0.77 +0.9 690,535 - RR42_RS03120 0.77 +0.0 690,536 + RR42_RS03120 0.77 +0.6 690,714 - -1.4 690,761 - +0.3 690,865 + RR42_RS03125 0.11 -0.4 690,865 + RR42_RS03125 0.11 +0.3 690,865 + RR42_RS03125 0.11 +0.7 690,866 - RR42_RS03125 0.11 -2.5 690,866 - RR42_RS03125 0.11 -0.7 690,866 - RR42_RS03125 0.11 +1.1 690,867 + RR42_RS03125 0.11 +0.0 690,868 - RR42_RS03125 0.11 -2.1 690,868 - RR42_RS03125 0.11 +0.6 690,868 - RR42_RS03125 0.11 -0.2 690,868 - RR42_RS03125 0.11 +0.8 690,868 - RR42_RS03125 0.11 +1.9 690,874 - RR42_RS03125 0.13 -2.6 690,876 + RR42_RS03125 0.13 -2.7 690,876 + RR42_RS03125 0.13 +1.3 690,876 + RR42_RS03125 0.13 -0.1 690,900 + RR42_RS03125 0.18 +0.0 690,901 - RR42_RS03125 0.18 -0.7 690,906 + RR42_RS03125 0.19 +0.2 690,906 + RR42_RS03125 0.19 -0.4 690,907 - RR42_RS03125 0.19 -0.7 690,907 - RR42_RS03125 0.19 +0.1 690,954 + RR42_RS03125 0.29 -2.6 690,984 + RR42_RS03125 0.35 -0.6 690,984 + RR42_RS03125 0.35 -0.4 690,985 - RR42_RS03125 0.35 -2.1 690,985 - RR42_RS03125 0.35 +0.5 691,026 + RR42_RS03125 0.43 +0.3 691,048 - RR42_RS03125 0.48 -2.0 691,048 - RR42_RS03125 0.48 -0.1 691,050 + RR42_RS03125 0.48 +0.3 691,051 - RR42_RS03125 0.48 -0.5 691,101 + RR42_RS03125 0.58 -0.2 691,102 - RR42_RS03125 0.59 -1.0 691,102 - RR42_RS03125 0.59 -2.1 691,102 - RR42_RS03125 0.59 +1.5 691,102 - RR42_RS03125 0.59 +0.3 691,102 - RR42_RS03125 0.59 -0.2 691,102 - RR42_RS03125 0.59 -0.5 691,109 + RR42_RS03125 0.60 +0.3 691,109 + RR42_RS03125 0.60 -0.0 691,110 - RR42_RS03125 0.60 -0.0
Or see this region's nucleotide sequence