Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02505 and RR42_RS02510 are separated by 195 nucleotides RR42_RS02510 and RR42_RS02515 are separated by 18 nucleotides
RR42_RS02505: RR42_RS02505 - acyl-CoA dehydrogenase, at 561,906 to 563,693
_RS02505
RR42_RS02510: RR42_RS02510 - 3-hydroxyacyl-CoA dehydrogenase, at 563,889 to 566,315
_RS02510
RR42_RS02515: RR42_RS02515 - acetyl-CoA acetyltransferase, at 566,334 to 567,533
_RS02515
Position (kb) 563
564
565
566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 563.196 kb on + strand, within RR42_RS02505 at 563.196 kb on + strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.197 kb on - strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.226 kb on + strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.227 kb on - strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.475 kb on + strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.476 kb on - strand, within RR42_RS02505 at 563.508 kb on + strand, within RR42_RS02505 at 563.528 kb on + strand at 563.568 kb on + strand at 563.569 kb on - strand at 563.569 kb on - strand at 563.598 kb on + strand at 563.598 kb on + strand at 563.712 kb on + strand at 563.724 kb on - strand at 563.740 kb on - strand at 563.740 kb on - strand at 563.772 kb on - strand at 563.803 kb on + strand at 563.804 kb on - strand at 563.878 kb on + strand at 563.878 kb on + strand at 564.117 kb on + strand at 564.202 kb on - strand, within RR42_RS02510 at 564.252 kb on - strand, within RR42_RS02510 at 564.345 kb on + strand, within RR42_RS02510 at 564.346 kb on - strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.528 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.690 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.762 kb on + strand, within RR42_RS02510 at 564.813 kb on + strand, within RR42_RS02510 at 564.955 kb on - strand, within RR42_RS02510 at 564.955 kb on - strand, within RR42_RS02510 at 564.966 kb on + strand, within RR42_RS02510 at 565.248 kb on + strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.340 kb on - strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.635 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.737 kb on + strand, within RR42_RS02510 at 565.738 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 565.846 kb on - strand, within RR42_RS02510 at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.358 kb on + strand at 566.359 kb on - strand at 566.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 563,196 + RR42_RS02505 0.72 -0.8 563,196 + RR42_RS02505 0.72 -0.1 563,197 - RR42_RS02505 0.72 +0.6 563,197 - RR42_RS02505 0.72 -0.7 563,226 + RR42_RS02505 0.74 +1.0 563,226 + RR42_RS02505 0.74 -0.1 563,227 - RR42_RS02505 0.74 +0.7 563,227 - RR42_RS02505 0.74 -2.0 563,475 + RR42_RS02505 0.88 +0.8 563,475 + RR42_RS02505 0.88 +1.3 563,476 - RR42_RS02505 0.88 +1.6 563,476 - RR42_RS02505 0.88 -1.0 563,476 - RR42_RS02505 0.88 +1.9 563,508 + RR42_RS02505 0.90 -0.1 563,528 + +0.4 563,568 + +0.3 563,569 - +0.2 563,569 - +1.1 563,598 + +0.6 563,598 + +0.6 563,712 + -0.0 563,724 - +0.6 563,740 - -0.0 563,740 - -1.0 563,772 - +2.3 563,803 + -0.5 563,804 - -0.1 563,878 + -1.7 563,878 + -0.0 564,117 + +1.8 564,202 - RR42_RS02510 0.13 -0.2 564,252 - RR42_RS02510 0.15 -0.3 564,345 + RR42_RS02510 0.19 -0.5 564,346 - RR42_RS02510 0.19 -0.1 564,528 + RR42_RS02510 0.26 +0.9 564,528 + RR42_RS02510 0.26 -1.4 564,690 + RR42_RS02510 0.33 -0.3 564,690 + RR42_RS02510 0.33 -1.1 564,690 + RR42_RS02510 0.33 -0.5 564,762 + RR42_RS02510 0.36 +0.1 564,762 + RR42_RS02510 0.36 -0.2 564,762 + RR42_RS02510 0.36 -0.4 564,813 + RR42_RS02510 0.38 -0.5 564,955 - RR42_RS02510 0.44 -0.6 564,955 - RR42_RS02510 0.44 -0.1 564,966 + RR42_RS02510 0.44 -1.0 565,248 + RR42_RS02510 0.56 +0.3 565,340 - RR42_RS02510 0.60 +0.3 565,340 - RR42_RS02510 0.60 -0.3 565,635 + RR42_RS02510 0.72 -3.2 565,635 + RR42_RS02510 0.72 -1.9 565,737 + RR42_RS02510 0.76 -0.7 565,737 + RR42_RS02510 0.76 +1.2 565,737 + RR42_RS02510 0.76 -0.3 565,737 + RR42_RS02510 0.76 -0.4 565,738 - RR42_RS02510 0.76 -0.4 565,846 - RR42_RS02510 0.81 -0.2 565,846 - RR42_RS02510 0.81 +0.5 566,358 + -0.0 566,358 + +1.1 566,358 + -0.9 566,358 + +1.4 566,358 + +0.6 566,359 - +0.5 566,359 - +0.2
Or see this region's nucleotide sequence