Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02365 and RR42_RS02370 are separated by 10 nucleotides RR42_RS02370 and RR42_RS02375 are separated by 398 nucleotides RR42_RS02375 and RR42_RS02380 are separated by 158 nucleotides
RR42_RS02365: RR42_RS02365 - carbon monoxide dehydrogenase, at 529,543 to 531,924
_RS02365
RR42_RS02370: RR42_RS02370 - carbon monoxide dehydrogenase, at 531,935 to 532,417
_RS02370
RR42_RS02375: RR42_RS02375 - membrane protein, at 532,816 to 533,394
_RS02375
RR42_RS02380: RR42_RS02380 - FAD-linked oxidase, at 533,553 to 537,527
_RS02380
Position (kb) 532
533
534 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 531.839 kb on + strand at 531.866 kb on + strand at 531.866 kb on + strand at 531.867 kb on - strand at 532.364 kb on + strand, within RR42_RS02370 at 532.521 kb on + strand at 532.522 kb on - strand at 532.545 kb on + strand at 532.554 kb on - strand at 532.626 kb on + strand at 532.627 kb on - strand at 532.627 kb on - strand at 532.627 kb on - strand at 532.731 kb on - strand at 532.731 kb on - strand at 532.813 kb on + strand at 532.813 kb on + strand at 532.813 kb on + strand at 532.814 kb on - strand at 532.815 kb on + strand at 532.815 kb on + strand at 532.815 kb on + strand at 532.819 kb on - strand at 532.946 kb on - strand, within RR42_RS02375 at 532.947 kb on + strand, within RR42_RS02375 at 532.948 kb on - strand, within RR42_RS02375 at 532.948 kb on - strand, within RR42_RS02375 at 532.948 kb on - strand, within RR42_RS02375 at 533.161 kb on + strand, within RR42_RS02375 at 533.162 kb on - strand, within RR42_RS02375 at 533.284 kb on + strand, within RR42_RS02375 at 533.284 kb on + strand, within RR42_RS02375 at 533.284 kb on + strand, within RR42_RS02375 at 533.285 kb on - strand, within RR42_RS02375 at 533.285 kb on - strand, within RR42_RS02375 at 533.285 kb on - strand, within RR42_RS02375 at 533.312 kb on - strand, within RR42_RS02375 at 533.312 kb on - strand, within RR42_RS02375 at 533.392 kb on + strand at 533.392 kb on + strand at 533.392 kb on + strand at 533.392 kb on + strand at 533.392 kb on + strand at 533.487 kb on - strand at 533.487 kb on - strand at 533.626 kb on - strand at 533.745 kb on + strand at 534.001 kb on - strand, within RR42_RS02380 at 534.120 kb on + strand, within RR42_RS02380 at 534.229 kb on - strand, within RR42_RS02380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 531,839 + +0.9 531,866 + +0.1 531,866 + +1.8 531,867 - -1.2 532,364 + RR42_RS02370 0.89 +0.1 532,521 + -0.4 532,522 - +1.3 532,545 + -0.2 532,554 - -1.7 532,626 + +1.1 532,627 - -0.4 532,627 - -0.1 532,627 - +0.2 532,731 - +0.0 532,731 - -3.0 532,813 + -1.2 532,813 + -0.4 532,813 + -0.4 532,814 - -1.5 532,815 + -0.1 532,815 + -0.4 532,815 + -0.9 532,819 - -2.3 532,946 - RR42_RS02375 0.22 -0.5 532,947 + RR42_RS02375 0.23 -0.0 532,948 - RR42_RS02375 0.23 +0.2 532,948 - RR42_RS02375 0.23 -2.1 532,948 - RR42_RS02375 0.23 -1.5 533,161 + RR42_RS02375 0.60 -1.3 533,162 - RR42_RS02375 0.60 -1.1 533,284 + RR42_RS02375 0.81 +0.4 533,284 + RR42_RS02375 0.81 -0.0 533,284 + RR42_RS02375 0.81 -1.1 533,285 - RR42_RS02375 0.81 -1.0 533,285 - RR42_RS02375 0.81 -1.4 533,285 - RR42_RS02375 0.81 -1.1 533,312 - RR42_RS02375 0.86 -0.6 533,312 - RR42_RS02375 0.86 -0.8 533,392 + +0.0 533,392 + -0.7 533,392 + -1.3 533,392 + -0.2 533,392 + -0.5 533,487 - -1.3 533,487 - -0.6 533,626 - -1.2 533,745 + -2.1 534,001 - RR42_RS02380 0.11 -2.3 534,120 + RR42_RS02380 0.14 -2.1 534,229 - RR42_RS02380 0.17 -1.1
Or see this region's nucleotide sequence