Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01835 and RR42_RS01840 are separated by 164 nucleotides RR42_RS01840 and RR42_RS01845 are separated by 117 nucleotides RR42_RS01845 and RR42_RS01850 are separated by 7 nucleotides
RR42_RS01835: RR42_RS01835 - glutaredoxin, at 416,293 to 416,550
_RS01835
RR42_RS01840: RR42_RS01840 - preprotein translocase subunit SecB, at 416,715 to 417,233
_RS01840
RR42_RS01845: RR42_RS01845 - glycerol-3-phosphate dehydrogenase, at 417,351 to 418,367
_RS01845
RR42_RS01850: RR42_RS01850 - ABC transporter substrate-binding protein, at 418,375 to 419,367
_RS01850
Position (kb)
417
418
419 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 416.464 kb on + strand, within RR42_RS01835 at 416.464 kb on + strand, within RR42_RS01835 at 416.596 kb on + strand at 416.596 kb on + strand at 416.596 kb on + strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.603 kb on + strand at 417.316 kb on - strand at 417.762 kb on + strand, within RR42_RS01845 at 417.762 kb on + strand, within RR42_RS01845 at 418.288 kb on + strand at 418.655 kb on - strand, within RR42_RS01850 at 418.656 kb on + strand, within RR42_RS01850 at 418.656 kb on + strand, within RR42_RS01850 at 418.656 kb on + strand, within RR42_RS01850 at 418.656 kb on + strand, within RR42_RS01850 at 418.656 kb on + strand, within RR42_RS01850 at 418.656 kb on + strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.657 kb on - strand, within RR42_RS01850 at 418.859 kb on - strand, within RR42_RS01850 at 418.859 kb on - strand, within RR42_RS01850 at 418.859 kb on - strand, within RR42_RS01850 at 418.859 kb on - strand, within RR42_RS01850 at 419.260 kb on + strand, within RR42_RS01850 at 419.260 kb on + strand, within RR42_RS01850 at 419.261 kb on - strand, within RR42_RS01850 at 419.261 kb on - strand, within RR42_RS01850 at 419.261 kb on - strand, within RR42_RS01850 at 419.366 kb on - strand at 419.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 416,464 + RR42_RS01835 0.66 +0.6 416,464 + RR42_RS01835 0.66 -0.0 416,596 + -1.2 416,596 + -0.6 416,596 + +0.5 416,597 - +1.4 416,597 - +0.9 416,597 - -0.5 416,597 - -0.0 416,601 + -2.0 416,601 + +0.8 416,601 + +2.1 416,601 + -0.5 416,601 + -1.4 416,601 + -0.3 416,601 + +0.2 416,603 + +1.9 417,316 - -0.9 417,762 + RR42_RS01845 0.40 -0.8 417,762 + RR42_RS01845 0.40 -1.6 418,288 + +1.2 418,655 - RR42_RS01850 0.28 -0.6 418,656 + RR42_RS01850 0.28 -2.7 418,656 + RR42_RS01850 0.28 +1.0 418,656 + RR42_RS01850 0.28 -0.5 418,656 + RR42_RS01850 0.28 -1.1 418,656 + RR42_RS01850 0.28 +0.5 418,656 + RR42_RS01850 0.28 -0.7 418,657 - RR42_RS01850 0.28 -0.4 418,657 - RR42_RS01850 0.28 -2.7 418,657 - RR42_RS01850 0.28 -0.6 418,657 - RR42_RS01850 0.28 +0.2 418,657 - RR42_RS01850 0.28 -0.1 418,657 - RR42_RS01850 0.28 +0.2 418,657 - RR42_RS01850 0.28 -1.2 418,657 - RR42_RS01850 0.28 -1.1 418,859 - RR42_RS01850 0.49 -0.4 418,859 - RR42_RS01850 0.49 +0.5 418,859 - RR42_RS01850 0.49 +0.0 418,859 - RR42_RS01850 0.49 -2.2 419,260 + RR42_RS01850 0.89 -0.1 419,260 + RR42_RS01850 0.89 +0.9 419,261 - RR42_RS01850 0.89 +0.3 419,261 - RR42_RS01850 0.89 +0.5 419,261 - RR42_RS01850 0.89 -0.2 419,366 - -1.1 419,366 - -0.4
Or see this region's nucleotide sequence