Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01810 and RR42_RS01815 are separated by 15 nucleotides RR42_RS01815 and RR42_RS01820 are separated by 62 nucleotides RR42_RS01820 and RR42_RS01825 are separated by 161 nucleotides
RR42_RS01810: RR42_RS01810 - tRNA 2-selenouridine synthase, at 411,153 to 412,277
_RS01810
RR42_RS01815: RR42_RS01815 - molybdopterin biosynthesis protein MoeB, at 412,293 to 413,060
_RS01815
RR42_RS01820: RR42_RS01820 - peptidase S41, at 413,123 to 414,712
_RS01820
RR42_RS01825: RR42_RS01825 - phosphoglyceromutase, at 414,874 to 415,620
_RS01825
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 412.167 kb on + strand at 412.167 kb on + strand at 412.167 kb on + strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.170 kb on + strand at 412.170 kb on + strand at 412.171 kb on - strand at 412.171 kb on - strand at 412.171 kb on - strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 413.626 kb on - strand, within RR42_RS01820 at 413.626 kb on - strand, within RR42_RS01820 at 413.626 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.632 kb on - strand, within RR42_RS01820 at 413.813 kb on - strand, within RR42_RS01820 at 413.887 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.550 kb on - strand, within RR42_RS01820 at 414.550 kb on - strand, within RR42_RS01820 at 414.680 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 415.664 kb on - strand at 415.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 412,167 + +0.9 412,167 + -0.4 412,167 + -1.2 412,168 - +1.6 412,168 - -0.8 412,168 - -0.3 412,168 - -0.4 412,168 - +1.1 412,170 + -1.4 412,170 + -0.8 412,171 - +0.5 412,171 - -0.4 412,171 - -0.1 412,182 + +0.6 412,182 + -0.1 412,182 + -0.4 412,182 + +2.0 412,183 - -0.2 412,183 - +0.5 412,183 - -0.2 412,183 - -0.8 412,183 - -0.0 412,183 - -1.7 412,183 - +0.4 413,626 - RR42_RS01820 0.32 -0.4 413,626 - RR42_RS01820 0.32 -0.9 413,626 - RR42_RS01820 0.32 +0.1 413,629 - RR42_RS01820 0.32 -0.4 413,629 - RR42_RS01820 0.32 -0.3 413,629 - RR42_RS01820 0.32 +0.5 413,632 - RR42_RS01820 0.32 -2.5 413,813 - RR42_RS01820 0.43 +0.1 413,887 - RR42_RS01820 0.48 -2.6 414,211 - RR42_RS01820 0.68 -1.0 414,211 - RR42_RS01820 0.68 -0.2 414,211 - RR42_RS01820 0.68 -0.7 414,550 - RR42_RS01820 0.90 -0.5 414,550 - RR42_RS01820 0.90 +0.2 414,680 - -0.2 414,793 - +1.1 414,793 - +0.2 414,793 - +0.3 414,793 - -0.8 414,793 - -0.5 414,794 + +0.1 414,794 + -3.0 414,794 + -0.4 414,794 + +0.3 414,794 + -0.3 414,794 + +0.7 414,794 + +0.7 414,794 + +1.0 414,795 - +0.5 414,795 - -0.3 414,795 - -0.5 414,795 - -1.2 414,795 - -0.0 414,795 - -0.1 414,795 - -1.1 414,795 - +1.4 415,664 - -0.5 415,685 - +0.4
Or see this region's nucleotide sequence