Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01395 and RR42_RS01400 are separated by 22 nucleotides RR42_RS01400 and RR42_RS01405 are separated by 153 nucleotides RR42_RS01405 and RR42_RS01410 are separated by 104 nucleotides
RR42_RS01395: RR42_RS01395 - twitching motility protein PilT, at 314,708 to 315,475
_RS01395
RR42_RS01400: RR42_RS01400 - 5-formyltetrahydrofolate cyclo-ligase, at 315,498 to 316,049
_RS01400
RR42_RS01405: RR42_RS01405 - lytic transglycosylase, at 316,203 to 318,155
_RS01405
RR42_RS01410: RR42_RS01410 - NAD-dependent dehydratase, at 318,260 to 319,285
_RS01410
Position (kb) 316
317
318
319 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.431 kb on - strand at 315.431 kb on - strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.474 kb on - strand at 315.644 kb on - strand, within RR42_RS01400 at 315.674 kb on + strand, within RR42_RS01400 at 315.675 kb on - strand, within RR42_RS01400 at 315.708 kb on + strand, within RR42_RS01400 at 315.709 kb on - strand, within RR42_RS01400 at 315.754 kb on + strand, within RR42_RS01400 at 315.755 kb on - strand, within RR42_RS01400 at 315.913 kb on + strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 316.119 kb on + strand at 316.120 kb on - strand at 316.216 kb on + strand at 316.216 kb on + strand at 316.217 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.282 kb on + strand at 316.283 kb on - strand at 316.431 kb on + strand, within RR42_RS01405 at 316.446 kb on + strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.545 kb on + strand, within RR42_RS01405 at 316.546 kb on - strand, within RR42_RS01405 at 316.678 kb on - strand, within RR42_RS01405 at 316.752 kb on + strand, within RR42_RS01405 at 316.756 kb on - strand, within RR42_RS01405 at 316.785 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.972 kb on - strand, within RR42_RS01405 at 317.077 kb on - strand, within RR42_RS01405 at 317.080 kb on - strand, within RR42_RS01405 at 317.238 kb on + strand, within RR42_RS01405 at 317.238 kb on + strand, within RR42_RS01405 at 317.238 kb on + strand, within RR42_RS01405 at 317.239 kb on - strand, within RR42_RS01405 at 317.239 kb on - strand, within RR42_RS01405 at 317.239 kb on - strand, within RR42_RS01405 at 317.245 kb on - strand, within RR42_RS01405 at 317.325 kb on + strand, within RR42_RS01405 at 317.325 kb on + strand, within RR42_RS01405 at 317.325 kb on + strand, within RR42_RS01405 at 317.449 kb on - strand, within RR42_RS01405 at 317.449 kb on - strand, within RR42_RS01405 at 317.538 kb on + strand, within RR42_RS01405 at 317.688 kb on + strand, within RR42_RS01405 at 317.689 kb on - strand, within RR42_RS01405 at 317.850 kb on - strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.868 kb on + strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.869 kb on - strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.870 kb on + strand, within RR42_RS01405 at 317.871 kb on - strand, within RR42_RS01405 at 317.871 kb on - strand, within RR42_RS01405 at 317.871 kb on - strand, within RR42_RS01405 at 317.876 kb on + strand, within RR42_RS01405 at 317.876 kb on + strand, within RR42_RS01405 at 317.877 kb on - strand, within RR42_RS01405 at 317.926 kb on - strand, within RR42_RS01405 at 318.030 kb on + strand at 318.030 kb on + strand at 318.031 kb on - strand at 318.031 kb on - strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.060 kb on + strand at 318.063 kb on + strand at 318.063 kb on + strand at 318.063 kb on + strand at 318.063 kb on + strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.064 kb on - strand at 318.162 kb on - strand at 318.279 kb on + strand at 318.714 kb on - strand, within RR42_RS01410 at 318.714 kb on - strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.894 kb on - strand, within RR42_RS01410 at 318.894 kb on - strand, within RR42_RS01410 at 318.959 kb on + strand, within RR42_RS01410 at 318.983 kb on + strand, within RR42_RS01410 at 318.984 kb on - strand, within RR42_RS01410 at 318.984 kb on - strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 315,430 + +1.6 315,430 + +0.6 315,430 + -1.3 315,430 + +1.0 315,431 - +0.0 315,431 - -1.0 315,473 + +0.6 315,473 + -0.5 315,473 + +0.2 315,473 + +0.2 315,474 - +0.7 315,644 - RR42_RS01400 0.26 -0.3 315,674 + RR42_RS01400 0.32 -0.4 315,675 - RR42_RS01400 0.32 -1.3 315,708 + RR42_RS01400 0.38 -0.0 315,709 - RR42_RS01400 0.38 -0.3 315,754 + RR42_RS01400 0.46 -0.8 315,755 - RR42_RS01400 0.47 +1.1 315,913 + RR42_RS01400 0.75 +0.6 315,914 - RR42_RS01400 0.75 +0.8 315,914 - RR42_RS01400 0.75 +0.0 316,119 + +0.5 316,120 - -0.7 316,216 + -1.2 316,216 + -0.2 316,217 - -1.9 316,219 - -1.5 316,219 - -2.2 316,219 - -0.5 316,282 + -0.5 316,283 - -1.8 316,431 + RR42_RS01405 0.12 -1.4 316,446 + RR42_RS01405 0.12 -1.1 316,447 - RR42_RS01405 0.12 -1.1 316,447 - RR42_RS01405 0.12 -0.5 316,447 - RR42_RS01405 0.12 -0.9 316,447 - RR42_RS01405 0.12 -1.4 316,455 + RR42_RS01405 0.13 +1.2 316,455 + RR42_RS01405 0.13 +0.4 316,545 + RR42_RS01405 0.18 -0.4 316,546 - RR42_RS01405 0.18 -1.8 316,678 - RR42_RS01405 0.24 -0.7 316,752 + RR42_RS01405 0.28 -1.2 316,756 - RR42_RS01405 0.28 -0.7 316,785 + RR42_RS01405 0.30 -1.0 316,857 + RR42_RS01405 0.33 -1.7 316,857 + RR42_RS01405 0.33 -0.7 316,857 + RR42_RS01405 0.33 +0.2 316,858 - RR42_RS01405 0.34 -1.3 316,858 - RR42_RS01405 0.34 -1.3 316,858 - RR42_RS01405 0.34 -1.4 316,858 - RR42_RS01405 0.34 -3.1 316,858 - RR42_RS01405 0.34 -0.7 316,858 - RR42_RS01405 0.34 -0.4 316,972 - RR42_RS01405 0.39 -1.1 317,077 - RR42_RS01405 0.45 +2.0 317,080 - RR42_RS01405 0.45 -0.8 317,238 + RR42_RS01405 0.53 -0.7 317,238 + RR42_RS01405 0.53 -1.2 317,238 + RR42_RS01405 0.53 -1.7 317,239 - RR42_RS01405 0.53 -0.3 317,239 - RR42_RS01405 0.53 -0.3 317,239 - RR42_RS01405 0.53 +1.2 317,245 - RR42_RS01405 0.53 -0.8 317,325 + RR42_RS01405 0.57 -1.7 317,325 + RR42_RS01405 0.57 -1.6 317,325 + RR42_RS01405 0.57 -1.4 317,449 - RR42_RS01405 0.64 +0.5 317,449 - RR42_RS01405 0.64 -0.9 317,538 + RR42_RS01405 0.68 -0.6 317,688 + RR42_RS01405 0.76 -0.8 317,689 - RR42_RS01405 0.76 -2.5 317,850 - RR42_RS01405 0.84 -0.1 317,868 + RR42_RS01405 0.85 -1.5 317,868 + RR42_RS01405 0.85 -2.1 317,868 + RR42_RS01405 0.85 +0.5 317,868 + RR42_RS01405 0.85 +1.2 317,868 + RR42_RS01405 0.85 -1.9 317,868 + RR42_RS01405 0.85 -1.4 317,869 - RR42_RS01405 0.85 +0.7 317,869 - RR42_RS01405 0.85 -1.6 317,869 - RR42_RS01405 0.85 -1.0 317,869 - RR42_RS01405 0.85 -1.1 317,869 - RR42_RS01405 0.85 -1.1 317,869 - RR42_RS01405 0.85 -0.5 317,869 - RR42_RS01405 0.85 -1.2 317,870 + RR42_RS01405 0.85 -1.5 317,870 + RR42_RS01405 0.85 -1.5 317,870 + RR42_RS01405 0.85 -0.1 317,870 + RR42_RS01405 0.85 -1.4 317,870 + RR42_RS01405 0.85 -3.7 317,870 + RR42_RS01405 0.85 -1.6 317,870 + RR42_RS01405 0.85 -2.3 317,870 + RR42_RS01405 0.85 -1.5 317,871 - RR42_RS01405 0.85 -1.7 317,871 - RR42_RS01405 0.85 -1.4 317,871 - RR42_RS01405 0.85 +0.1 317,876 + RR42_RS01405 0.86 -1.1 317,876 + RR42_RS01405 0.86 +0.6 317,877 - RR42_RS01405 0.86 -2.5 317,926 - RR42_RS01405 0.88 -3.2 318,030 + -1.4 318,030 + -1.6 318,031 - -0.6 318,031 - -2.3 318,060 + -3.2 318,060 + -1.6 318,060 + -1.7 318,060 + -0.1 318,060 + -1.4 318,060 + -1.8 318,060 + +0.1 318,063 + -0.8 318,063 + -1.1 318,063 + -1.3 318,063 + -1.2 318,064 - -1.9 318,064 - -1.5 318,064 - -0.6 318,064 - -2.3 318,064 - -0.8 318,162 - -0.2 318,279 + -0.4 318,714 - RR42_RS01410 0.44 -2.0 318,714 - RR42_RS01410 0.44 +0.9 318,893 + RR42_RS01410 0.62 +0.6 318,893 + RR42_RS01410 0.62 -0.8 318,893 + RR42_RS01410 0.62 +1.3 318,894 - RR42_RS01410 0.62 -0.2 318,894 - RR42_RS01410 0.62 +0.1 318,959 + RR42_RS01410 0.68 -1.4 318,983 + RR42_RS01410 0.70 -1.1 318,984 - RR42_RS01410 0.71 -1.1 318,984 - RR42_RS01410 0.71 +1.0 318,985 + RR42_RS01410 0.71 -0.5 318,985 + RR42_RS01410 0.71 -1.4 318,985 + RR42_RS01410 0.71 -1.0 318,985 + RR42_RS01410 0.71 +0.7 318,986 - RR42_RS01410 0.71 -0.1 318,986 - RR42_RS01410 0.71 -0.1 318,986 - RR42_RS01410 0.71 -1.1
Or see this region's nucleotide sequence