Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01175 and RR42_RS01180 are separated by 100 nucleotides RR42_RS01180 and RR42_RS01185 are separated by 157 nucleotides
RR42_RS01175: RR42_RS01175 - membrane protein, at 269,239 to 270,183
_RS01175
RR42_RS01180: RR42_RS01180 - acyl-CoA thioesterase, at 270,284 to 271,114
_RS01180
RR42_RS01185: RR42_RS01185 - muropeptide transporter, at 271,272 to 272,528
_RS01185
Position (kb) 270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 269.523 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.567 kb on - strand, within RR42_RS01175 at 269.596 kb on + strand, within RR42_RS01175 at 269.785 kb on + strand, within RR42_RS01175 at 269.956 kb on - strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 270.034 kb on + strand, within RR42_RS01175 at 270.387 kb on - strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.945 kb on - strand, within RR42_RS01180 at 271.103 kb on + strand at 271.103 kb on + strand at 271.103 kb on + strand at 271.103 kb on + strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.112 kb on + strand at 271.112 kb on + strand at 271.112 kb on + strand at 271.113 kb on - strand at 271.113 kb on - strand at 271.113 kb on - strand at 271.171 kb on - strand at 271.171 kb on - strand at 271.233 kb on - strand at 271.273 kb on + strand at 271.300 kb on + strand at 271.330 kb on - strand at 271.339 kb on - strand at 271.372 kb on + strand at 271.373 kb on - strand at 271.426 kb on + strand, within RR42_RS01185 at 271.427 kb on - strand, within RR42_RS01185 at 271.427 kb on - strand, within RR42_RS01185 at 271.610 kb on - strand, within RR42_RS01185 at 271.660 kb on + strand, within RR42_RS01185 at 271.660 kb on + strand, within RR42_RS01185 at 271.826 kb on - strand, within RR42_RS01185 at 271.996 kb on + strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 269,523 + RR42_RS01175 0.30 -0.5 269,566 + RR42_RS01175 0.35 +0.8 269,566 + RR42_RS01175 0.35 +0.6 269,566 + RR42_RS01175 0.35 +0.5 269,567 - RR42_RS01175 0.35 +0.3 269,596 + RR42_RS01175 0.38 -0.6 269,785 + RR42_RS01175 0.58 +0.1 269,956 - RR42_RS01175 0.76 -0.5 269,968 + RR42_RS01175 0.77 -1.4 269,968 + RR42_RS01175 0.77 -0.5 269,968 + RR42_RS01175 0.77 -0.5 269,968 + RR42_RS01175 0.77 +0.1 269,969 - RR42_RS01175 0.77 -1.4 269,969 - RR42_RS01175 0.77 -0.1 269,969 - RR42_RS01175 0.77 -0.0 269,969 - RR42_RS01175 0.77 +0.6 269,980 + RR42_RS01175 0.78 -1.0 269,980 + RR42_RS01175 0.78 -0.6 269,980 + RR42_RS01175 0.78 +1.8 269,980 + RR42_RS01175 0.78 +0.5 269,981 - RR42_RS01175 0.79 -0.6 269,981 - RR42_RS01175 0.79 -0.7 269,981 - RR42_RS01175 0.79 +0.9 269,981 - RR42_RS01175 0.79 +1.0 270,034 + RR42_RS01175 0.84 +1.2 270,387 - RR42_RS01180 0.12 +0.6 270,737 + RR42_RS01180 0.55 -0.2 270,737 + RR42_RS01180 0.55 +0.2 270,737 + RR42_RS01180 0.55 +2.0 270,944 + RR42_RS01180 0.79 +1.7 270,944 + RR42_RS01180 0.79 +0.5 270,944 + RR42_RS01180 0.79 -0.6 270,944 + RR42_RS01180 0.79 -0.4 270,945 - RR42_RS01180 0.80 -2.1 271,103 + +0.5 271,103 + +0.3 271,103 + +0.6 271,103 + +1.2 271,104 - -0.0 271,104 - -0.8 271,104 - +0.2 271,104 - +0.8 271,104 - -0.4 271,112 + -1.6 271,112 + -0.1 271,112 + +0.2 271,113 - -0.5 271,113 - -0.1 271,113 - +0.1 271,171 - -0.4 271,171 - -0.1 271,233 - -0.7 271,273 + +0.4 271,300 + +0.6 271,330 - -0.4 271,339 - -2.4 271,372 + -2.0 271,373 - -1.6 271,426 + RR42_RS01185 0.12 -1.8 271,427 - RR42_RS01185 0.12 -1.2 271,427 - RR42_RS01185 0.12 -1.0 271,610 - RR42_RS01185 0.27 -2.0 271,660 + RR42_RS01185 0.31 -2.2 271,660 + RR42_RS01185 0.31 -1.8 271,826 - RR42_RS01185 0.44 -2.4 271,996 + RR42_RS01185 0.58 -2.3 271,997 - RR42_RS01185 0.58 -1.0 271,997 - RR42_RS01185 0.58 -2.8
Or see this region's nucleotide sequence