Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS01105

Experiment: R2A with Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS01100 and RR42_RS01105 are separated by 94 nucleotidesRR42_RS01105 and RR42_RS01110 are separated by 0 nucleotidesRR42_RS01110 and RR42_RS01115 overlap by 4 nucleotidesRR42_RS01115 and RR42_RS01120 are separated by 90 nucleotides RR42_RS01100: RR42_RS01100 - LysR family transcriptional regulator, at 256,406 to 257,341 _RS01100 RR42_RS01105: RR42_RS01105 - membrane protein, at 257,436 to 257,948 _RS01105 RR42_RS01110: RR42_RS01110 - hypothetical protein, at 257,949 to 258,176 _RS01110 RR42_RS01115: RR42_RS01115 - hypothetical protein, at 258,173 to 258,400 _RS01115 RR42_RS01120: RR42_RS01120 - RNA polymerase sigma factor, at 258,491 to 259,072 _RS01120 Position (kb) 257 258Strain fitness (log2 ratio) -2 -1 0 1 2at 256.480 kb on + strandat 256.480 kb on + strandat 256.481 kb on - strandat 256.830 kb on + strand, within RR42_RS01100at 256.831 kb on - strand, within RR42_RS01100at 256.831 kb on - strand, within RR42_RS01100at 257.025 kb on + strand, within RR42_RS01100at 257.026 kb on - strand, within RR42_RS01100at 257.273 kb on - strandat 257.273 kb on - strandat 257.273 kb on - strandat 257.273 kb on - strandat 257.350 kb on + strandat 257.350 kb on + strandat 257.360 kb on - strandat 257.392 kb on - strandat 257.706 kb on + strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.707 kb on - strand, within RR42_RS01105at 257.712 kb on - strand, within RR42_RS01105at 257.712 kb on - strand, within RR42_RS01105at 257.712 kb on - strand, within RR42_RS01105at 257.757 kb on - strand, within RR42_RS01105at 258.391 kb on + strandat 258.392 kb on - strandat 258.552 kb on + strand, within RR42_RS01120at 258.553 kb on - strand, within RR42_RS01120at 258.553 kb on - strand, within RR42_RS01120at 258.571 kb on + strand, within RR42_RS01120at 258.571 kb on + strand, within RR42_RS01120at 258.572 kb on - strand, within RR42_RS01120at 258.572 kb on - strand, within RR42_RS01120at 258.599 kb on + strand, within RR42_RS01120at 258.620 kb on + strand, within RR42_RS01120at 258.620 kb on + strand, within RR42_RS01120at 258.748 kb on + strand, within RR42_RS01120at 258.749 kb on - strand, within RR42_RS01120at 258.753 kb on + strand, within RR42_RS01120at 258.753 kb on + strand, within RR42_RS01120at 258.753 kb on + strand, within RR42_RS01120at 258.754 kb on - strand, within RR42_RS01120at 258.872 kb on - strand, within RR42_RS01120at 258.872 kb on - strand, within RR42_RS01120at 258.906 kb on + strand, within RR42_RS01120at 258.906 kb on + strand, within RR42_RS01120at 258.906 kb on + strand, within RR42_RS01120at 258.907 kb on - strand, within RR42_RS01120

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml
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256,480 + -1.3
256,480 + -1.4
256,481 - -0.4
256,830 + RR42_RS01100 0.45 -0.4
256,831 - RR42_RS01100 0.45 +0.2
256,831 - RR42_RS01100 0.45 -0.1
257,025 + RR42_RS01100 0.66 -0.8
257,026 - RR42_RS01100 0.66 -2.2
257,273 - -0.8
257,273 - +1.5
257,273 - -0.6
257,273 - -0.1
257,350 + -1.1
257,350 + -0.2
257,360 - -0.5
257,392 - -0.4
257,706 + RR42_RS01105 0.53 -1.3
257,707 - RR42_RS01105 0.53 -0.5
257,707 - RR42_RS01105 0.53 +1.0
257,707 - RR42_RS01105 0.53 -0.5
257,707 - RR42_RS01105 0.53 +2.0
257,712 - RR42_RS01105 0.54 +2.0
257,712 - RR42_RS01105 0.54 -0.9
257,712 - RR42_RS01105 0.54 -2.0
257,757 - RR42_RS01105 0.63 +1.2
258,391 + +1.5
258,392 - +1.2
258,552 + RR42_RS01120 0.10 -0.7
258,553 - RR42_RS01120 0.11 +0.3
258,553 - RR42_RS01120 0.11 +0.8
258,571 + RR42_RS01120 0.14 +0.7
258,571 + RR42_RS01120 0.14 -1.2
258,572 - RR42_RS01120 0.14 -0.6
258,572 - RR42_RS01120 0.14 -0.5
258,599 + RR42_RS01120 0.19 -1.5
258,620 + RR42_RS01120 0.22 -0.0
258,620 + RR42_RS01120 0.22 -0.1
258,748 + RR42_RS01120 0.44 +0.4
258,749 - RR42_RS01120 0.44 +0.5
258,753 + RR42_RS01120 0.45 +0.8
258,753 + RR42_RS01120 0.45 -1.9
258,753 + RR42_RS01120 0.45 -1.1
258,754 - RR42_RS01120 0.45 -2.3
258,872 - RR42_RS01120 0.65 -1.0
258,872 - RR42_RS01120 0.65 +0.0
258,906 + RR42_RS01120 0.71 +0.0
258,906 + RR42_RS01120 0.71 -1.9
258,906 + RR42_RS01120 0.71 -0.8
258,907 - RR42_RS01120 0.71 -0.3

Or see this region's nucleotide sequence