Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01025 and RR42_RS01030 are separated by 253 nucleotides RR42_RS01030 and RR42_RS01035 overlap by 2 nucleotides RR42_RS01035 and RR42_RS01040 are separated by 282 nucleotides
RR42_RS01025: RR42_RS01025 - GTPase, at 242,771 to 244,066
_RS01025
RR42_RS01030: RR42_RS01030 - molecular chaperone DnaK, at 244,320 to 244,739
_RS01030
RR42_RS01035: RR42_RS01035 - hypothetical protein, at 244,738 to 245,046
_RS01035
RR42_RS01040: RR42_RS01040 - cobalamin biosynthesis protein CobW, at 245,329 to 246,417
_RS01040
Position (kb) 244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 243.563 kb on + strand, within RR42_RS01025 at 243.564 kb on - strand, within RR42_RS01025 at 243.564 kb on - strand, within RR42_RS01025 at 243.564 kb on - strand, within RR42_RS01025 at 243.680 kb on - strand, within RR42_RS01025 at 243.786 kb on + strand, within RR42_RS01025 at 243.787 kb on - strand, within RR42_RS01025 at 244.041 kb on - strand at 244.122 kb on + strand at 244.189 kb on + strand at 244.190 kb on - strand at 244.190 kb on - strand at 244.195 kb on + strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 245.187 kb on - strand at 245.187 kb on - strand at 245.212 kb on - strand at 245.212 kb on - strand at 245.212 kb on - strand at 245.216 kb on + strand at 245.216 kb on + strand at 245.216 kb on + strand at 245.217 kb on - strand at 245.217 kb on - strand at 245.240 kb on - strand at 245.240 kb on - strand at 245.270 kb on - strand at 245.270 kb on - strand at 245.496 kb on + strand, within RR42_RS01040 at 245.496 kb on + strand, within RR42_RS01040 at 245.496 kb on + strand, within RR42_RS01040 at 245.497 kb on - strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.503 kb on + strand, within RR42_RS01040 at 245.503 kb on + strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.513 kb on + strand, within RR42_RS01040 at 245.534 kb on + strand, within RR42_RS01040 at 245.534 kb on + strand, within RR42_RS01040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 243,563 + RR42_RS01025 0.61 -1.5 243,564 - RR42_RS01025 0.61 -2.5 243,564 - RR42_RS01025 0.61 -1.4 243,564 - RR42_RS01025 0.61 -0.6 243,680 - RR42_RS01025 0.70 -0.7 243,786 + RR42_RS01025 0.78 +0.9 243,787 - RR42_RS01025 0.78 -2.6 244,041 - -1.2 244,122 + -1.0 244,189 + -0.4 244,190 - -0.0 244,190 - -0.7 244,195 + -0.4 244,196 - -0.5 244,196 - -0.1 244,196 - -0.9 244,196 - -1.1 244,196 - -0.1 245,187 - +1.1 245,187 - -1.6 245,212 - -1.6 245,212 - +0.9 245,212 - -0.4 245,216 + -1.5 245,216 + -1.6 245,216 + -0.7 245,217 - +0.5 245,217 - +1.1 245,240 - +0.3 245,240 - -1.5 245,270 - +0.9 245,270 - +1.0 245,496 + RR42_RS01040 0.15 +0.4 245,496 + RR42_RS01040 0.15 +0.2 245,496 + RR42_RS01040 0.15 +0.3 245,497 - RR42_RS01040 0.15 -0.0 245,498 + RR42_RS01040 0.16 -0.7 245,498 + RR42_RS01040 0.16 +0.0 245,498 + RR42_RS01040 0.16 -0.9 245,498 + RR42_RS01040 0.16 +0.3 245,498 + RR42_RS01040 0.16 -1.6 245,498 + RR42_RS01040 0.16 -0.2 245,498 + RR42_RS01040 0.16 -0.7 245,498 + RR42_RS01040 0.16 -0.2 245,498 + RR42_RS01040 0.16 -1.6 245,498 + RR42_RS01040 0.16 -1.0 245,498 + RR42_RS01040 0.16 -1.0 245,498 + RR42_RS01040 0.16 +1.2 245,498 + RR42_RS01040 0.16 -0.4 245,498 + RR42_RS01040 0.16 -0.7 245,498 + RR42_RS01040 0.16 +1.0 245,499 - RR42_RS01040 0.16 -0.8 245,499 - RR42_RS01040 0.16 +0.7 245,499 - RR42_RS01040 0.16 +1.1 245,499 - RR42_RS01040 0.16 +0.2 245,503 + RR42_RS01040 0.16 -0.7 245,503 + RR42_RS01040 0.16 -0.3 245,504 - RR42_RS01040 0.16 +2.2 245,504 - RR42_RS01040 0.16 +1.2 245,504 - RR42_RS01040 0.16 +0.5 245,504 - RR42_RS01040 0.16 -3.0 245,504 - RR42_RS01040 0.16 +0.2 245,513 + RR42_RS01040 0.17 -0.9 245,534 + RR42_RS01040 0.19 +0.3 245,534 + RR42_RS01040 0.19 -0.4
Or see this region's nucleotide sequence