Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00830 and RR42_RS00835 are separated by 91 nucleotides RR42_RS00835 and RR42_RS00840 are separated by 168 nucleotides RR42_RS00840 and RR42_RS00845 overlap by 4 nucleotides RR42_RS00845 and RR42_RS00850 overlap by 4 nucleotides
RR42_RS00830: RR42_RS00830 - cell division protein FtsN, at 203,416 to 204,192
_RS00830
RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922
_RS00835
RR42_RS00840: RR42_RS00840 - short-chain dehydrogenase, at 205,091 to 205,903
_RS00840
RR42_RS00845: RR42_RS00845 - metal ABC transporter substrate-binding protein, at 205,900 to 206,736
_RS00845
RR42_RS00850: RR42_RS00850 - hypothetical protein, at 206,733 to 206,993
_RS00850
Position (kb)
205
206 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 204.180 kb on + strand at 204.181 kb on - strand at 204.191 kb on - strand at 204.191 kb on - strand at 204.200 kb on + strand at 204.250 kb on + strand at 204.250 kb on + strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.384 kb on - strand, within RR42_RS00835 at 204.450 kb on - strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.788 kb on + strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.830 kb on + strand, within RR42_RS00835 at 204.830 kb on + strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.921 kb on - strand at 204.921 kb on - strand at 205.157 kb on + strand at 205.158 kb on - strand at 205.562 kb on + strand, within RR42_RS00840 at 205.563 kb on - strand, within RR42_RS00840 at 205.832 kb on + strand at 206.135 kb on + strand, within RR42_RS00845 at 206.327 kb on + strand, within RR42_RS00845 at 206.327 kb on + strand, within RR42_RS00845 at 206.327 kb on + strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.363 kb on - strand, within RR42_RS00845 at 206.363 kb on - strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.409 kb on + strand, within RR42_RS00845 at 206.410 kb on - strand, within RR42_RS00845 at 206.506 kb on - strand, within RR42_RS00845 at 206.544 kb on + strand, within RR42_RS00845 at 206.544 kb on + strand, within RR42_RS00845 at 206.590 kb on + strand, within RR42_RS00845 at 206.707 kb on + strand at 206.707 kb on + strand at 206.707 kb on + strand at 206.708 kb on - strand at 206.708 kb on - strand at 206.708 kb on - strand at 206.734 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 204,180 + -1.4 204,181 - -1.7 204,191 - -0.2 204,191 - -1.1 204,200 + +0.1 204,250 + +0.2 204,250 + -2.5 204,251 - -2.2 204,251 - -1.1 204,251 - -0.9 204,251 - -2.4 204,251 - +1.2 204,251 - -3.4 204,251 - -1.4 204,251 - -1.5 204,384 - RR42_RS00835 0.16 -1.2 204,450 - RR42_RS00835 0.26 -1.0 204,731 + RR42_RS00835 0.70 -1.1 204,731 + RR42_RS00835 0.70 -0.9 204,731 + RR42_RS00835 0.70 -0.2 204,732 - RR42_RS00835 0.70 -1.6 204,732 - RR42_RS00835 0.70 -2.5 204,732 - RR42_RS00835 0.70 -0.0 204,732 - RR42_RS00835 0.70 -0.4 204,732 - RR42_RS00835 0.70 -0.0 204,732 - RR42_RS00835 0.70 -0.9 204,732 - RR42_RS00835 0.70 +0.6 204,732 - RR42_RS00835 0.70 -1.6 204,788 + RR42_RS00835 0.79 -0.7 204,789 - RR42_RS00835 0.79 -1.1 204,789 - RR42_RS00835 0.79 -0.6 204,789 - RR42_RS00835 0.79 -3.6 204,830 + RR42_RS00835 0.85 +0.2 204,830 + RR42_RS00835 0.85 -0.6 204,831 - RR42_RS00835 0.86 -1.7 204,831 - RR42_RS00835 0.86 +1.2 204,831 - RR42_RS00835 0.86 -0.3 204,831 - RR42_RS00835 0.86 -1.5 204,831 - RR42_RS00835 0.86 +0.6 204,831 - RR42_RS00835 0.86 -0.7 204,921 - -0.6 204,921 - +1.0 205,157 + -0.0 205,158 - -0.8 205,562 + RR42_RS00840 0.58 -0.6 205,563 - RR42_RS00840 0.58 -1.1 205,832 + +0.3 206,135 + RR42_RS00845 0.28 +1.0 206,327 + RR42_RS00845 0.51 -0.4 206,327 + RR42_RS00845 0.51 +0.6 206,327 + RR42_RS00845 0.51 +1.1 206,328 - RR42_RS00845 0.51 +1.0 206,328 - RR42_RS00845 0.51 -0.1 206,328 - RR42_RS00845 0.51 +0.3 206,328 - RR42_RS00845 0.51 -0.6 206,328 - RR42_RS00845 0.51 +0.3 206,328 - RR42_RS00845 0.51 +0.5 206,362 + RR42_RS00845 0.55 +0.1 206,362 + RR42_RS00845 0.55 -0.4 206,362 + RR42_RS00845 0.55 +0.9 206,362 + RR42_RS00845 0.55 -0.9 206,363 - RR42_RS00845 0.55 +0.6 206,363 - RR42_RS00845 0.55 -0.9 206,407 + RR42_RS00845 0.61 -0.9 206,407 + RR42_RS00845 0.61 -0.4 206,407 + RR42_RS00845 0.61 -1.8 206,407 + RR42_RS00845 0.61 +0.2 206,407 + RR42_RS00845 0.61 +0.4 206,407 + RR42_RS00845 0.61 -0.5 206,407 + RR42_RS00845 0.61 -0.1 206,407 + RR42_RS00845 0.61 +1.2 206,407 + RR42_RS00845 0.61 -0.5 206,408 - RR42_RS00845 0.61 -1.4 206,408 - RR42_RS00845 0.61 -1.4 206,408 - RR42_RS00845 0.61 +0.3 206,408 - RR42_RS00845 0.61 -0.0 206,408 - RR42_RS00845 0.61 +0.1 206,408 - RR42_RS00845 0.61 -0.2 206,408 - RR42_RS00845 0.61 -0.6 206,408 - RR42_RS00845 0.61 -0.7 206,409 + RR42_RS00845 0.61 +1.4 206,410 - RR42_RS00845 0.61 +0.6 206,506 - RR42_RS00845 0.72 -1.0 206,544 + RR42_RS00845 0.77 -1.8 206,544 + RR42_RS00845 0.77 +0.9 206,590 + RR42_RS00845 0.82 +1.0 206,707 + +0.1 206,707 + +0.3 206,707 + -0.3 206,708 - -0.6 206,708 - -0.4 206,708 - +1.1 206,734 + -0.6
Or see this region's nucleotide sequence