Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00635 and RR42_RS00640 are separated by 677 nucleotides RR42_RS00640 and RR42_RS00645 overlap by 4 nucleotides
RR42_RS00635: RR42_RS00635 - lipoyl synthase, at 164,909 to 165,904
_RS00635
RR42_RS00640: RR42_RS00640 - allophanate hydrolase, at 166,582 to 167,232
_RS00640
RR42_RS00645: RR42_RS00645 - allophanate hydrolase, at 167,229 to 168,251
_RS00645
Position (kb) 166
167
168 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 165.991 kb on + strand at 165.991 kb on + strand at 165.991 kb on + strand at 165.992 kb on - strand at 165.992 kb on - strand at 165.992 kb on - strand at 165.995 kb on + strand at 165.996 kb on - strand at 165.996 kb on - strand at 165.996 kb on - strand at 166.043 kb on + strand at 166.043 kb on + strand at 166.044 kb on - strand at 166.044 kb on - strand at 166.397 kb on + strand at 166.411 kb on + strand at 166.412 kb on - strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.449 kb on + strand at 166.467 kb on - strand at 166.528 kb on + strand at 166.529 kb on - strand at 166.529 kb on - strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.653 kb on - strand, within RR42_RS00640 at 166.653 kb on - strand, within RR42_RS00640 at 166.653 kb on - strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.669 kb on - strand, within RR42_RS00640 at 166.951 kb on + strand, within RR42_RS00640 at 166.960 kb on + strand, within RR42_RS00640 at 166.960 kb on + strand, within RR42_RS00640 at 166.961 kb on - strand, within RR42_RS00640 at 166.961 kb on - strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.992 kb on + strand, within RR42_RS00640 at 166.992 kb on + strand, within RR42_RS00640 at 166.993 kb on - strand, within RR42_RS00640 at 167.095 kb on + strand, within RR42_RS00640 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.563 kb on - strand, within RR42_RS00645 at 168.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 165,991 + +0.7 165,991 + +0.2 165,991 + +0.7 165,992 - -1.0 165,992 - +0.1 165,992 - -1.0 165,995 + -2.3 165,996 - -1.3 165,996 - -0.4 165,996 - -0.5 166,043 + -0.8 166,043 + +1.2 166,044 - -0.0 166,044 - -0.4 166,397 + -0.1 166,411 + -1.1 166,412 - -0.5 166,424 + -1.1 166,424 + +0.0 166,424 + +1.3 166,424 + +1.6 166,449 + -0.9 166,467 - -0.7 166,528 + +0.2 166,529 - +1.2 166,529 - +0.2 166,546 + +0.2 166,546 + +0.9 166,546 + -0.5 166,546 + +0.2 166,546 + +1.7 166,653 - RR42_RS00640 0.11 +0.3 166,653 - RR42_RS00640 0.11 +1.0 166,653 - RR42_RS00640 0.11 +0.7 166,658 + RR42_RS00640 0.12 +0.7 166,658 + RR42_RS00640 0.12 +0.5 166,658 + RR42_RS00640 0.12 -0.3 166,659 - RR42_RS00640 0.12 +2.0 166,659 - RR42_RS00640 0.12 +0.2 166,659 - RR42_RS00640 0.12 -0.6 166,659 - RR42_RS00640 0.12 +1.2 166,659 - RR42_RS00640 0.12 +1.3 166,659 - RR42_RS00640 0.12 -1.5 166,668 + RR42_RS00640 0.13 -0.7 166,668 + RR42_RS00640 0.13 +0.3 166,668 + RR42_RS00640 0.13 +1.0 166,669 - RR42_RS00640 0.13 +0.2 166,951 + RR42_RS00640 0.57 -0.4 166,960 + RR42_RS00640 0.58 +0.4 166,960 + RR42_RS00640 0.58 -1.4 166,961 - RR42_RS00640 0.58 +0.2 166,961 - RR42_RS00640 0.58 +0.1 166,990 + RR42_RS00640 0.63 +0.3 166,990 + RR42_RS00640 0.63 +0.2 166,990 + RR42_RS00640 0.63 +0.1 166,990 + RR42_RS00640 0.63 -1.1 166,991 - RR42_RS00640 0.63 +0.7 166,991 - RR42_RS00640 0.63 -0.2 166,991 - RR42_RS00640 0.63 +0.2 166,992 + RR42_RS00640 0.63 -0.2 166,992 + RR42_RS00640 0.63 +0.1 166,993 - RR42_RS00640 0.63 +2.1 167,095 + RR42_RS00640 0.79 -0.3 167,562 + RR42_RS00645 0.33 -3.1 167,562 + RR42_RS00645 0.33 -0.1 167,562 + RR42_RS00645 0.33 -0.6 167,562 + RR42_RS00645 0.33 -0.2 167,563 - RR42_RS00645 0.33 +0.2 168,157 - -0.3
Or see this region's nucleotide sequence