Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00445 and RR42_RS00450 are separated by 351 nucleotides RR42_RS00450 and RR42_RS00455 are separated by 15 nucleotides RR42_RS00455 and RR42_RS00460 are separated by 62 nucleotides
RR42_RS00445: RR42_RS00445 - membrane protein, at 115,610 to 116,698
_RS00445
RR42_RS00450: RR42_RS00450 - cell division topological specificity factor MinE, at 117,050 to 117,304
_RS00450
RR42_RS00455: RR42_RS00455 - cell division inhibitor MinD, at 117,320 to 118,135
_RS00455
RR42_RS00460: RR42_RS00460 - septum formation inhibitor, at 118,198 to 119,013
_RS00460
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 116.346 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.451 kb on + strand, within RR42_RS00445 at 116.451 kb on + strand, within RR42_RS00445 at 116.611 kb on + strand at 116.616 kb on + strand at 116.616 kb on + strand at 116.616 kb on + strand at 116.617 kb on - strand at 116.643 kb on + strand at 116.665 kb on + strand at 116.666 kb on - strand at 116.666 kb on - strand at 116.712 kb on + strand at 116.712 kb on + strand at 116.713 kb on - strand at 116.756 kb on - strand at 116.767 kb on - strand at 116.810 kb on + strand at 116.810 kb on + strand at 116.810 kb on + strand at 116.811 kb on - strand at 116.811 kb on - strand at 116.811 kb on - strand at 116.811 kb on - strand at 116.833 kb on + strand at 116.834 kb on - strand at 116.912 kb on + strand at 116.921 kb on - strand at 116.938 kb on + strand at 117.322 kb on - strand at 117.322 kb on - strand at 117.363 kb on + strand at 117.420 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.589 kb on - strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.789 kb on + strand, within RR42_RS00455 at 117.789 kb on + strand, within RR42_RS00455 at 117.790 kb on - strand, within RR42_RS00455 at 117.945 kb on - strand, within RR42_RS00455 at 117.945 kb on - strand, within RR42_RS00455 at 117.963 kb on + strand, within RR42_RS00455 at 118.094 kb on + strand at 118.199 kb on + strand at 118.200 kb on - strand at 118.200 kb on - strand at 118.200 kb on - strand at 118.301 kb on + strand, within RR42_RS00460 at 118.406 kb on + strand, within RR42_RS00460 at 118.407 kb on - strand, within RR42_RS00460 at 118.407 kb on - strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.416 kb on + strand, within RR42_RS00460 at 118.445 kb on + strand, within RR42_RS00460 at 118.484 kb on + strand, within RR42_RS00460 at 118.485 kb on - strand, within RR42_RS00460 at 119.013 kb on + strand at 119.013 kb on + strand at 119.013 kb on + strand at 119.013 kb on + strand at 119.014 kb on - strand at 119.042 kb on + strand at 119.043 kb on - strand at 119.074 kb on - strand at 119.126 kb on + strand at 119.126 kb on + strand at 119.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 116,346 + RR42_RS00445 0.68 +0.4 116,394 + RR42_RS00445 0.72 -0.1 116,394 + RR42_RS00445 0.72 +0.6 116,394 + RR42_RS00445 0.72 +0.2 116,394 + RR42_RS00445 0.72 +0.2 116,394 + RR42_RS00445 0.72 -0.1 116,394 + RR42_RS00445 0.72 +0.3 116,394 + RR42_RS00445 0.72 -1.0 116,394 + RR42_RS00445 0.72 +0.6 116,395 - RR42_RS00445 0.72 -0.8 116,395 - RR42_RS00445 0.72 +0.4 116,395 - RR42_RS00445 0.72 -0.2 116,395 - RR42_RS00445 0.72 +0.1 116,395 - RR42_RS00445 0.72 +0.4 116,395 - RR42_RS00445 0.72 +0.2 116,395 - RR42_RS00445 0.72 +1.7 116,395 - RR42_RS00445 0.72 +1.0 116,451 + RR42_RS00445 0.77 +0.4 116,451 + RR42_RS00445 0.77 +2.5 116,611 + +0.4 116,616 + +1.6 116,616 + +1.2 116,616 + +0.4 116,617 - +0.2 116,643 + +0.6 116,665 + +0.3 116,666 - +0.5 116,666 - -0.1 116,712 + +0.6 116,712 + +0.2 116,713 - -0.6 116,756 - +2.8 116,767 - -0.2 116,810 + -1.6 116,810 + +0.6 116,810 + -0.1 116,811 - -1.0 116,811 - +1.9 116,811 - +0.2 116,811 - +0.4 116,833 + +0.1 116,834 - -1.4 116,912 + -0.2 116,921 - +0.7 116,938 + -0.2 117,322 - -1.5 117,322 - -1.6 117,363 + -0.6 117,420 + RR42_RS00455 0.12 -0.1 117,588 + RR42_RS00455 0.33 -1.1 117,588 + RR42_RS00455 0.33 +0.2 117,588 + RR42_RS00455 0.33 -3.0 117,588 + RR42_RS00455 0.33 -1.1 117,589 - RR42_RS00455 0.33 -1.1 117,735 + RR42_RS00455 0.51 -1.4 117,735 + RR42_RS00455 0.51 -1.0 117,735 + RR42_RS00455 0.51 -0.6 117,735 + RR42_RS00455 0.51 -0.5 117,736 - RR42_RS00455 0.51 -1.9 117,736 - RR42_RS00455 0.51 -0.3 117,736 - RR42_RS00455 0.51 -4.2 117,789 + RR42_RS00455 0.57 -0.6 117,789 + RR42_RS00455 0.57 -0.3 117,790 - RR42_RS00455 0.58 -0.2 117,945 - RR42_RS00455 0.77 +0.4 117,945 - RR42_RS00455 0.77 -1.0 117,963 + RR42_RS00455 0.79 -0.8 118,094 + -2.1 118,199 + -2.9 118,200 - +1.2 118,200 - -1.9 118,200 - -1.3 118,301 + RR42_RS00460 0.13 -1.0 118,406 + RR42_RS00460 0.25 -1.9 118,407 - RR42_RS00460 0.26 -1.1 118,407 - RR42_RS00460 0.26 -2.9 118,410 + RR42_RS00460 0.26 -0.4 118,410 + RR42_RS00460 0.26 -0.7 118,410 + RR42_RS00460 0.26 -0.8 118,411 - RR42_RS00460 0.26 -1.7 118,411 - RR42_RS00460 0.26 -1.0 118,411 - RR42_RS00460 0.26 +0.2 118,411 - RR42_RS00460 0.26 +0.5 118,411 - RR42_RS00460 0.26 -1.5 118,416 + RR42_RS00460 0.27 -1.0 118,445 + RR42_RS00460 0.30 -3.1 118,484 + RR42_RS00460 0.35 -1.6 118,485 - RR42_RS00460 0.35 -0.7 119,013 + -1.5 119,013 + -0.9 119,013 + +0.2 119,013 + -0.9 119,014 - +0.4 119,042 + -1.1 119,043 - -2.3 119,074 - -1.5 119,126 + +1.4 119,126 + +0.0 119,127 - +0.4
Or see this region's nucleotide sequence