Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00025 and RR42_RS00030 are separated by 3 nucleotides RR42_RS00030 and RR42_RS00035 are separated by 136 nucleotides RR42_RS00035 and RR42_RS00040 are separated by 213 nucleotides
RR42_RS00025: RR42_RS00025 - GTPase, at 7,996 to 10,740
_RS00025
RR42_RS00030: RR42_RS00030 - hypothetical protein, at 10,744 to 13,032
_RS00030
RR42_RS00035: RR42_RS00035 - hypothetical protein, at 13,169 to 13,756
_RS00035
RR42_RS00040: RR42_RS00040 - recombinase, at 13,970 to 14,593
_RS00040
Position (kb)
10
11
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 9.783 kb on - strand, within RR42_RS00025 at 9.863 kb on + strand, within RR42_RS00025 at 9.863 kb on + strand, within RR42_RS00025 at 9.953 kb on - strand, within RR42_RS00025 at 9.953 kb on - strand, within RR42_RS00025 at 9.990 kb on - strand, within RR42_RS00025 at 10.010 kb on + strand, within RR42_RS00025 at 10.010 kb on + strand, within RR42_RS00025 at 10.011 kb on - strand, within RR42_RS00025 at 10.086 kb on + strand, within RR42_RS00025 at 10.130 kb on + strand, within RR42_RS00025 at 10.159 kb on - strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.162 kb on + strand, within RR42_RS00025 at 10.163 kb on - strand, within RR42_RS00025 at 10.163 kb on - strand, within RR42_RS00025 at 10.163 kb on - strand, within RR42_RS00025 at 10.251 kb on + strand, within RR42_RS00025 at 10.357 kb on - strand, within RR42_RS00025 at 10.388 kb on + strand, within RR42_RS00025 at 10.388 kb on + strand, within RR42_RS00025 at 10.389 kb on - strand, within RR42_RS00025 at 10.396 kb on + strand, within RR42_RS00025 at 10.397 kb on - strand, within RR42_RS00025 at 10.397 kb on - strand, within RR42_RS00025 at 10.398 kb on + strand, within RR42_RS00025 at 10.399 kb on - strand, within RR42_RS00025 at 10.399 kb on - strand, within RR42_RS00025 at 10.468 kb on - strand at 10.504 kb on - strand at 10.615 kb on - strand at 10.615 kb on - strand at 10.615 kb on - strand at 10.615 kb on - strand at 10.643 kb on + strand at 10.644 kb on - strand at 10.664 kb on + strand at 10.664 kb on + strand at 10.706 kb on + strand at 10.707 kb on - strand at 10.707 kb on - strand at 10.750 kb on - strand at 10.805 kb on + strand at 10.814 kb on + strand at 10.814 kb on + strand at 10.836 kb on + strand at 10.836 kb on + strand at 10.965 kb on + strand at 10.965 kb on + strand at 10.965 kb on + strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 10.966 kb on - strand at 11.042 kb on + strand, within RR42_RS00030 at 11.112 kb on - strand, within RR42_RS00030 at 11.131 kb on + strand, within RR42_RS00030 at 11.132 kb on - strand, within RR42_RS00030 at 11.132 kb on - strand, within RR42_RS00030 at 11.143 kb on + strand, within RR42_RS00030 at 11.236 kb on - strand, within RR42_RS00030 at 11.236 kb on - strand, within RR42_RS00030 at 11.313 kb on + strand, within RR42_RS00030 at 11.365 kb on - strand, within RR42_RS00030 at 11.388 kb on - strand, within RR42_RS00030 at 11.613 kb on + strand, within RR42_RS00030 at 11.613 kb on + strand, within RR42_RS00030 at 11.614 kb on - strand, within RR42_RS00030 at 11.694 kb on + strand, within RR42_RS00030 at 11.695 kb on - strand, within RR42_RS00030 at 11.761 kb on - strand, within RR42_RS00030 at 11.767 kb on + strand at 11.767 kb on + strand, within RR42_RS00030 at 11.768 kb on - strand, within RR42_RS00030 at 11.768 kb on - strand, within RR42_RS00030 at 11.821 kb on + strand, within RR42_RS00030 at 11.822 kb on - strand, within RR42_RS00030 at 11.854 kb on + strand, within RR42_RS00030 at 11.855 kb on - strand, within RR42_RS00030 at 11.860 kb on + strand, within RR42_RS00030 at 11.860 kb on + strand, within RR42_RS00030 at 11.861 kb on - strand, within RR42_RS00030 at 11.861 kb on - strand, within RR42_RS00030 at 11.879 kb on + strand, within RR42_RS00030 at 11.880 kb on - strand, within RR42_RS00030 at 11.955 kb on + strand, within RR42_RS00030 at 11.955 kb on + strand, within RR42_RS00030 at 11.956 kb on - strand, within RR42_RS00030 at 12.002 kb on + strand, within RR42_RS00030 at 12.003 kb on - strand, within RR42_RS00030 at 12.052 kb on + strand, within RR42_RS00030 at 12.053 kb on - strand, within RR42_RS00030 at 12.053 kb on - strand, within RR42_RS00030 at 12.074 kb on + strand, within RR42_RS00030 at 12.075 kb on - strand, within RR42_RS00030 at 12.106 kb on + strand, within RR42_RS00030 at 12.313 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.347 kb on - strand, within RR42_RS00030 at 12.390 kb on + strand, within RR42_RS00030 at 12.401 kb on - strand, within RR42_RS00030 at 12.401 kb on - strand, within RR42_RS00030 at 12.450 kb on + strand, within RR42_RS00030 at 12.450 kb on + strand, within RR42_RS00030 at 12.450 kb on + strand, within RR42_RS00030 at 12.463 kb on - strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.513 kb on - strand, within RR42_RS00030 at 12.565 kb on + strand, within RR42_RS00030 at 12.566 kb on - strand, within RR42_RS00030 at 12.566 kb on - strand, within RR42_RS00030 at 12.681 kb on + strand, within RR42_RS00030 at 12.681 kb on + strand, within RR42_RS00030 at 12.681 kb on + strand, within RR42_RS00030 at 12.682 kb on - strand, within RR42_RS00030 at 12.688 kb on + strand, within RR42_RS00030 at 12.689 kb on - strand, within RR42_RS00030 at 12.689 kb on - strand, within RR42_RS00030 at 12.689 kb on - strand, within RR42_RS00030 at 12.740 kb on + strand, within RR42_RS00030 at 12.741 kb on - strand, within RR42_RS00030 at 12.743 kb on + strand, within RR42_RS00030 at 12.744 kb on - strand, within RR42_RS00030 at 12.744 kb on - strand, within RR42_RS00030 at 12.744 kb on - strand, within RR42_RS00030 at 12.758 kb on + strand, within RR42_RS00030 at 12.758 kb on + strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.766 kb on + strand, within RR42_RS00030 at 12.821 kb on + strand at 12.824 kb on + strand at 12.825 kb on - strand at 12.874 kb on + strand at 12.874 kb on + strand at 12.874 kb on + strand at 12.875 kb on - strand at 12.884 kb on + strand at 12.884 kb on + strand at 12.885 kb on - strand at 12.885 kb on - strand at 12.885 kb on - strand at 12.954 kb on + strand at 12.973 kb on + strand at 12.973 kb on + strand at 12.973 kb on + strand at 12.974 kb on - strand at 13.079 kb on + strand at 13.080 kb on - strand at 13.127 kb on + strand at 13.127 kb on + strand at 13.128 kb on - strand at 13.128 kb on - strand at 13.583 kb on + strand, within RR42_RS00035 at 13.639 kb on + strand, within RR42_RS00035 at 13.640 kb on - strand, within RR42_RS00035 at 13.684 kb on - strand, within RR42_RS00035 at 13.686 kb on + strand, within RR42_RS00035 at 13.686 kb on + strand, within RR42_RS00035 at 13.686 kb on + strand, within RR42_RS00035 at 13.687 kb on - strand, within RR42_RS00035 at 13.792 kb on - strand at 13.792 kb on - strand at 13.879 kb on - strand at 13.879 kb on - strand at 13.879 kb on - strand at 13.901 kb on + strand at 13.901 kb on + strand at 13.902 kb on - strand at 13.902 kb on - strand at 13.902 kb on - strand at 13.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 9,783 - RR42_RS00025 0.65 -0.2 9,863 + RR42_RS00025 0.68 -2.0 9,863 + RR42_RS00025 0.68 +1.2 9,953 - RR42_RS00025 0.71 -0.2 9,953 - RR42_RS00025 0.71 -0.0 9,990 - RR42_RS00025 0.73 +0.5 10,010 + RR42_RS00025 0.73 -1.6 10,010 + RR42_RS00025 0.73 -0.0 10,011 - RR42_RS00025 0.73 -1.1 10,086 + RR42_RS00025 0.76 +0.4 10,130 + RR42_RS00025 0.78 -0.5 10,159 - RR42_RS00025 0.79 -0.3 10,162 + RR42_RS00025 0.79 -1.3 10,162 + RR42_RS00025 0.79 -0.5 10,162 + RR42_RS00025 0.79 +0.0 10,162 + RR42_RS00025 0.79 +0.5 10,162 + RR42_RS00025 0.79 +0.3 10,163 - RR42_RS00025 0.79 -0.5 10,163 - RR42_RS00025 0.79 -0.3 10,163 - RR42_RS00025 0.79 -0.1 10,251 + RR42_RS00025 0.82 +0.7 10,357 - RR42_RS00025 0.86 +1.2 10,388 + RR42_RS00025 0.87 +1.2 10,388 + RR42_RS00025 0.87 +0.8 10,389 - RR42_RS00025 0.87 -2.8 10,396 + RR42_RS00025 0.87 +0.2 10,397 - RR42_RS00025 0.87 -0.6 10,397 - RR42_RS00025 0.87 -0.0 10,398 + RR42_RS00025 0.88 -0.8 10,399 - RR42_RS00025 0.88 +0.0 10,399 - RR42_RS00025 0.88 -0.9 10,468 - +0.2 10,504 - +1.0 10,615 - -0.3 10,615 - -0.9 10,615 - +2.7 10,615 - +1.4 10,643 + -0.3 10,644 - +0.0 10,664 + +1.0 10,664 + -0.5 10,706 + -0.8 10,707 - -0.2 10,707 - +1.4 10,750 - +0.9 10,805 + -0.8 10,814 + -0.0 10,814 + -0.3 10,836 + -0.2 10,836 + -0.1 10,965 + -0.2 10,965 + +1.5 10,965 + -0.5 10,966 - -1.1 10,966 - -0.2 10,966 - +0.4 10,966 - -0.3 10,966 - -0.7 10,966 - +0.9 11,042 + RR42_RS00030 0.13 -2.2 11,112 - RR42_RS00030 0.16 +0.0 11,131 + RR42_RS00030 0.17 -0.3 11,132 - RR42_RS00030 0.17 +0.1 11,132 - RR42_RS00030 0.17 -0.0 11,143 + RR42_RS00030 0.17 +0.1 11,236 - RR42_RS00030 0.21 +0.7 11,236 - RR42_RS00030 0.21 +0.5 11,313 + RR42_RS00030 0.25 -1.1 11,365 - RR42_RS00030 0.27 -0.2 11,388 - RR42_RS00030 0.28 -2.3 11,613 + RR42_RS00030 0.38 -0.2 11,613 + RR42_RS00030 0.38 +0.4 11,614 - RR42_RS00030 0.38 +0.2 11,694 + RR42_RS00030 0.42 +0.1 11,695 - RR42_RS00030 0.42 -1.8 11,761 - RR42_RS00030 0.44 +0.0 11,767 + +0.2 11,767 + RR42_RS00030 0.45 +0.1 11,768 - RR42_RS00030 0.45 -0.2 11,768 - RR42_RS00030 0.45 -0.3 11,821 + RR42_RS00030 0.47 +1.2 11,822 - RR42_RS00030 0.47 -0.7 11,854 + RR42_RS00030 0.48 +0.7 11,855 - RR42_RS00030 0.49 -0.3 11,860 + RR42_RS00030 0.49 -0.5 11,860 + RR42_RS00030 0.49 +1.5 11,861 - RR42_RS00030 0.49 -0.2 11,861 - RR42_RS00030 0.49 -1.3 11,879 + RR42_RS00030 0.50 +0.9 11,880 - RR42_RS00030 0.50 -0.1 11,955 + RR42_RS00030 0.53 -0.5 11,955 + RR42_RS00030 0.53 +0.3 11,956 - RR42_RS00030 0.53 +0.2 12,002 + RR42_RS00030 0.55 +0.1 12,003 - RR42_RS00030 0.55 -0.6 12,052 + RR42_RS00030 0.57 -1.3 12,053 - RR42_RS00030 0.57 +0.5 12,053 - RR42_RS00030 0.57 -0.9 12,074 + RR42_RS00030 0.58 +4.9 12,075 - RR42_RS00030 0.58 +0.6 12,106 + RR42_RS00030 0.60 +0.7 12,313 + RR42_RS00030 0.69 -0.0 12,346 + RR42_RS00030 0.70 -0.2 12,346 + RR42_RS00030 0.70 +0.0 12,346 + RR42_RS00030 0.70 +0.2 12,346 + RR42_RS00030 0.70 +0.3 12,347 - RR42_RS00030 0.70 -0.5 12,390 + RR42_RS00030 0.72 -0.1 12,401 - RR42_RS00030 0.72 -0.2 12,401 - RR42_RS00030 0.72 -0.3 12,450 + RR42_RS00030 0.75 +0.8 12,450 + RR42_RS00030 0.75 -0.9 12,450 + RR42_RS00030 0.75 -0.1 12,463 - RR42_RS00030 0.75 -0.0 12,512 + RR42_RS00030 0.77 -1.2 12,512 + RR42_RS00030 0.77 -1.4 12,512 + RR42_RS00030 0.77 -0.4 12,512 + RR42_RS00030 0.77 +0.4 12,513 - RR42_RS00030 0.77 -0.7 12,565 + RR42_RS00030 0.80 -0.7 12,566 - RR42_RS00030 0.80 -0.2 12,566 - RR42_RS00030 0.80 -0.1 12,681 + RR42_RS00030 0.85 -0.1 12,681 + RR42_RS00030 0.85 -0.8 12,681 + RR42_RS00030 0.85 +0.3 12,682 - RR42_RS00030 0.85 +0.2 12,688 + RR42_RS00030 0.85 -0.8 12,689 - RR42_RS00030 0.85 +0.5 12,689 - RR42_RS00030 0.85 +0.5 12,689 - RR42_RS00030 0.85 +1.2 12,740 + RR42_RS00030 0.87 +0.2 12,741 - RR42_RS00030 0.87 +0.1 12,743 + RR42_RS00030 0.87 -0.1 12,744 - RR42_RS00030 0.87 -1.0 12,744 - RR42_RS00030 0.87 -0.6 12,744 - RR42_RS00030 0.87 -1.0 12,758 + RR42_RS00030 0.88 +0.7 12,758 + RR42_RS00030 0.88 +0.4 12,759 - RR42_RS00030 0.88 -0.3 12,759 - RR42_RS00030 0.88 -0.2 12,759 - RR42_RS00030 0.88 +0.7 12,759 - RR42_RS00030 0.88 +1.0 12,766 + RR42_RS00030 0.88 -1.4 12,821 + +2.2 12,824 + -2.5 12,825 - -0.1 12,874 + +1.2 12,874 + -0.1 12,874 + -0.5 12,875 - -1.6 12,884 + +0.1 12,884 + +0.4 12,885 - -2.1 12,885 - +0.2 12,885 - -1.2 12,954 + -1.2 12,973 + +0.8 12,973 + -1.6 12,973 + -0.1 12,974 - -0.2 13,079 + -0.9 13,080 - -0.1 13,127 + +0.3 13,127 + -1.4 13,128 - +0.1 13,128 - -1.3 13,583 + RR42_RS00035 0.70 -0.3 13,639 + RR42_RS00035 0.80 +1.0 13,640 - RR42_RS00035 0.80 -0.7 13,684 - RR42_RS00035 0.88 -0.1 13,686 + RR42_RS00035 0.88 -0.4 13,686 + RR42_RS00035 0.88 +1.0 13,686 + RR42_RS00035 0.88 -0.0 13,687 - RR42_RS00035 0.88 +0.5 13,792 - -0.9 13,792 - +0.2 13,879 - +0.2 13,879 - -0.0 13,879 - -0.7 13,901 + -0.7 13,901 + -1.9 13,902 - +0.7 13,902 - -0.9 13,902 - -0.1 13,902 - +0.7
Or see this region's nucleotide sequence