Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_716 and Pf1N1B4_717 are separated by 27 nucleotides Pf1N1B4_717 and Pf1N1B4_718 are separated by 14 nucleotides
Pf1N1B4_716 - Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites, at 816,248 to 817,675
_716
Pf1N1B4_717 - Iron-regulated protein A precursor, at 817,703 to 818,767
_717
Pf1N1B4_718 - outer membrane component of uptake system, probably for ferrous iron (from data) , at 818,782 to 819,882
_718
Position (kb)
817
818
819 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 816.740 kb on - strand, within Pf1N1B4_716 at 817.093 kb on + strand, within Pf1N1B4_716 at 817.229 kb on + strand, within Pf1N1B4_716 at 817.229 kb on + strand, within Pf1N1B4_716 at 817.229 kb on + strand, within Pf1N1B4_716 at 817.231 kb on + strand, within Pf1N1B4_716 at 817.398 kb on - strand, within Pf1N1B4_716 at 817.673 kb on + strand at 817.676 kb on + strand at 817.677 kb on - strand at 817.823 kb on + strand, within Pf1N1B4_717 at 817.824 kb on - strand, within Pf1N1B4_717 at 818.101 kb on - strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.580 kb on - strand, within Pf1N1B4_717 at 818.672 kb on + strand at 818.672 kb on + strand at 818.672 kb on + strand at 818.672 kb on + strand at 818.918 kb on - strand, within Pf1N1B4_718 at 819.011 kb on + strand, within Pf1N1B4_718 at 819.050 kb on - strand, within Pf1N1B4_718 at 819.115 kb on + strand, within Pf1N1B4_718 at 819.115 kb on + strand, within Pf1N1B4_718 at 819.154 kb on + strand, within Pf1N1B4_718 at 819.155 kb on - strand, within Pf1N1B4_718 at 819.205 kb on + strand, within Pf1N1B4_718 at 819.205 kb on + strand, within Pf1N1B4_718 at 819.206 kb on - strand, within Pf1N1B4_718 at 819.206 kb on - strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.386 kb on + strand, within Pf1N1B4_718 at 819.604 kb on + strand, within Pf1N1B4_718 at 819.604 kb on + strand, within Pf1N1B4_718 at 819.605 kb on - strand, within Pf1N1B4_718 at 819.678 kb on + strand, within Pf1N1B4_718 at 819.679 kb on - strand, within Pf1N1B4_718 at 819.679 kb on - strand, within Pf1N1B4_718 at 819.704 kb on + strand, within Pf1N1B4_718 at 819.705 kb on - strand, within Pf1N1B4_718
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 816,740 - Pf1N1B4_716 0.34 +0.3 817,093 + Pf1N1B4_716 0.59 -2.5 817,229 + Pf1N1B4_716 0.69 -2.8 817,229 + Pf1N1B4_716 0.69 -1.7 817,229 + Pf1N1B4_716 0.69 -2.1 817,231 + Pf1N1B4_716 0.69 -1.0 817,398 - Pf1N1B4_716 0.81 -2.0 817,673 + +0.3 817,676 + +1.9 817,677 - -1.3 817,823 + Pf1N1B4_717 0.11 -2.2 817,824 - Pf1N1B4_717 0.11 -1.6 818,101 - Pf1N1B4_717 0.37 +0.0 818,129 + Pf1N1B4_717 0.40 -0.4 818,129 + Pf1N1B4_717 0.40 -1.0 818,129 + +0.7 818,129 + Pf1N1B4_717 0.40 -1.0 818,129 + Pf1N1B4_717 0.40 -2.2 818,129 + +1.3 818,129 + Pf1N1B4_717 0.40 -0.5 818,129 + Pf1N1B4_717 0.40 -1.2 818,129 + Pf1N1B4_717 0.40 -0.7 818,129 + Pf1N1B4_717 0.40 -1.6 818,130 - Pf1N1B4_717 0.40 -0.3 818,130 - Pf1N1B4_717 0.40 -0.0 818,130 - Pf1N1B4_717 0.40 -0.7 818,130 - Pf1N1B4_717 0.40 +1.3 818,130 - Pf1N1B4_717 0.40 -0.3 818,130 - Pf1N1B4_717 0.40 +0.7 818,130 - Pf1N1B4_717 0.40 -0.7 818,130 - Pf1N1B4_717 0.40 -0.9 818,183 + Pf1N1B4_717 0.45 -1.4 818,183 + Pf1N1B4_717 0.45 -1.3 818,183 + Pf1N1B4_717 0.45 +1.6 818,183 + Pf1N1B4_717 0.45 -1.3 818,184 - Pf1N1B4_717 0.45 -0.4 818,184 - Pf1N1B4_717 0.45 -3.5 818,184 - Pf1N1B4_717 0.45 +0.3 818,580 - Pf1N1B4_717 0.82 -2.3 818,672 + -1.3 818,672 + +0.3 818,672 + -1.0 818,672 + -1.5 818,918 - Pf1N1B4_718 0.12 -0.7 819,011 + Pf1N1B4_718 0.21 -0.3 819,050 - Pf1N1B4_718 0.24 -0.5 819,115 + Pf1N1B4_718 0.30 -2.5 819,115 + Pf1N1B4_718 0.30 -0.5 819,154 + Pf1N1B4_718 0.34 -1.9 819,155 - Pf1N1B4_718 0.34 +1.3 819,205 + Pf1N1B4_718 0.38 +0.3 819,205 + Pf1N1B4_718 0.38 -1.0 819,206 - Pf1N1B4_718 0.39 -1.0 819,206 - Pf1N1B4_718 0.39 -2.0 819,207 + Pf1N1B4_718 0.39 +2.3 819,207 + Pf1N1B4_718 0.39 +0.7 819,207 + Pf1N1B4_718 0.39 -1.2 819,207 + Pf1N1B4_718 0.39 -0.7 819,207 + Pf1N1B4_718 0.39 +0.3 819,207 + Pf1N1B4_718 0.39 -0.5 819,207 + Pf1N1B4_718 0.39 -0.3 819,208 - Pf1N1B4_718 0.39 -2.3 819,208 - Pf1N1B4_718 0.39 -1.3 819,208 - Pf1N1B4_718 0.39 +0.7 819,208 - Pf1N1B4_718 0.39 -1.9 819,386 + Pf1N1B4_718 0.55 -0.7 819,604 + Pf1N1B4_718 0.75 -1.7 819,604 + Pf1N1B4_718 0.75 -1.3 819,605 - Pf1N1B4_718 0.75 -0.0 819,678 + Pf1N1B4_718 0.81 -0.3 819,679 - Pf1N1B4_718 0.81 -0.9 819,679 - Pf1N1B4_718 0.81 -1.0 819,704 + Pf1N1B4_718 0.84 +0.5 819,705 - Pf1N1B4_718 0.84 -1.0
Or see this region's nucleotide sequence