Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3232 and Pf1N1B4_3233 overlap by 1 nucleotides Pf1N1B4_3233 and Pf1N1B4_3234 overlap by 1 nucleotides Pf1N1B4_3234 and Pf1N1B4_3235 are separated by 51 nucleotides Pf1N1B4_3235 and Pf1N1B4_3236 are separated by 1 nucleotides
Pf1N1B4_3232 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12), at 3,619,249 to 3,620,106
_3232
Pf1N1B4_3233 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12), at 3,620,106 to 3,620,885
_3233
Pf1N1B4_3234 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,620,885 to 3,622,087
_3234
Pf1N1B4_3235 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 3,622,139 to 3,622,843
_3235
Pf1N1B4_3236 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 3,622,845 to 3,623,411
_3236
Position (kb)
3620
3621
3622
3623 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3619.920 kb on - strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.284 kb on + strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.524 kb on + strand, within Pf1N1B4_3233 at 3620.525 kb on - strand, within Pf1N1B4_3233 at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.085 kb on - strand, within Pf1N1B4_3234 at 3621.726 kb on + strand at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.727 kb on - strand, within Pf1N1B4_3234 at 3621.982 kb on - strand at 3622.104 kb on + strand at 3622.105 kb on - strand at 3622.116 kb on + strand at 3622.119 kb on + strand at 3622.119 kb on + strand at 3622.119 kb on + strand at 3622.120 kb on - strand at 3622.138 kb on - strand at 3622.138 kb on - strand at 3622.190 kb on + strand at 3622.190 kb on + strand at 3622.191 kb on - strand at 3622.206 kb on - strand at 3622.365 kb on + strand at 3622.365 kb on + strand, within Pf1N1B4_3235 at 3622.365 kb on + strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.692 kb on - strand, within Pf1N1B4_3235 at 3622.692 kb on - strand, within Pf1N1B4_3235 at 3622.703 kb on + strand, within Pf1N1B4_3235 at 3622.778 kb on + strand at 3622.779 kb on - strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.842 kb on - strand at 3622.842 kb on - strand at 3622.842 kb on - strand at 3622.884 kb on + strand at 3622.885 kb on - strand at 3622.885 kb on - strand at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 3,619,920 - Pf1N1B4_3232 0.78 +0.6 3,619,978 + Pf1N1B4_3232 0.85 -0.3 3,619,978 + Pf1N1B4_3232 0.85 -1.3 3,619,978 + -1.3 3,619,979 - Pf1N1B4_3232 0.85 -1.9 3,619,979 - Pf1N1B4_3232 0.85 +1.0 3,620,038 + -1.7 3,620,038 + -0.2 3,620,038 + -0.5 3,620,038 + +0.8 3,620,038 + +0.3 3,620,038 + +0.5 3,620,038 + -0.1 3,620,039 - -1.3 3,620,039 - -1.3 3,620,039 - +0.9 3,620,039 - +0.0 3,620,104 + -0.4 3,620,104 + -1.9 3,620,104 + -0.0 3,620,105 - +0.3 3,620,105 - +0.3 3,620,105 - -0.7 3,620,112 + -0.0 3,620,112 + -0.3 3,620,112 + -1.2 3,620,113 - +2.3 3,620,113 - -1.1 3,620,113 - +1.6 3,620,113 - -1.0 3,620,113 - -1.0 3,620,113 - -0.5 3,620,113 - -1.3 3,620,239 + Pf1N1B4_3233 0.17 +0.6 3,620,239 + -0.0 3,620,239 + Pf1N1B4_3233 0.17 -1.6 3,620,239 + Pf1N1B4_3233 0.17 +0.9 3,620,240 - Pf1N1B4_3233 0.17 +0.5 3,620,240 - Pf1N1B4_3233 0.17 +0.3 3,620,240 - Pf1N1B4_3233 0.17 +0.9 3,620,250 + Pf1N1B4_3233 0.18 +0.3 3,620,250 + Pf1N1B4_3233 0.18 +0.6 3,620,250 + Pf1N1B4_3233 0.18 -0.5 3,620,250 + Pf1N1B4_3233 0.18 +0.6 3,620,250 + Pf1N1B4_3233 0.18 +0.3 3,620,251 - Pf1N1B4_3233 0.19 +0.9 3,620,251 - Pf1N1B4_3233 0.19 -0.6 3,620,251 - Pf1N1B4_3233 0.19 -0.3 3,620,284 + Pf1N1B4_3233 0.23 -1.0 3,620,455 - Pf1N1B4_3233 0.45 -0.7 3,620,455 - Pf1N1B4_3233 0.45 +0.2 3,620,524 + Pf1N1B4_3233 0.54 +0.5 3,620,525 - Pf1N1B4_3233 0.54 -1.3 3,620,897 + -1.5 3,620,897 + -1.2 3,620,897 + +1.7 3,620,897 + -1.3 3,620,897 + -0.6 3,620,897 + +1.7 3,620,897 + -1.2 3,620,897 + -1.5 3,620,898 - -0.3 3,620,898 - -1.9 3,620,898 - +0.6 3,620,898 - +0.3 3,620,899 + -1.0 3,620,899 + -0.6 3,620,899 + -2.4 3,620,899 + -0.7 3,620,899 + -1.2 3,620,899 + -0.3 3,620,900 - -0.0 3,620,900 - -1.7 3,620,900 - -2.7 3,621,084 + Pf1N1B4_3234 0.17 +1.7 3,621,084 + Pf1N1B4_3234 0.17 -1.9 3,621,084 + Pf1N1B4_3234 0.17 +0.3 3,621,085 - Pf1N1B4_3234 0.17 -0.7 3,621,726 + -0.7 3,621,726 + Pf1N1B4_3234 0.70 -0.1 3,621,726 + Pf1N1B4_3234 0.70 -1.1 3,621,726 + Pf1N1B4_3234 0.70 +0.7 3,621,727 - Pf1N1B4_3234 0.70 -1.5 3,621,982 - +0.3 3,622,104 + -0.4 3,622,105 - +0.5 3,622,116 + +0.9 3,622,119 + +2.0 3,622,119 + -1.3 3,622,119 + -0.0 3,622,120 - -0.3 3,622,138 - -0.0 3,622,138 - +1.7 3,622,190 + -0.6 3,622,190 + +0.3 3,622,191 - -0.1 3,622,206 - -0.3 3,622,365 + -0.3 3,622,365 + Pf1N1B4_3235 0.32 -1.2 3,622,365 + Pf1N1B4_3235 0.32 -0.2 3,622,366 - Pf1N1B4_3235 0.32 +1.9 3,622,366 - Pf1N1B4_3235 0.32 -0.7 3,622,366 - Pf1N1B4_3235 0.32 -0.0 3,622,664 + Pf1N1B4_3235 0.74 -0.7 3,622,664 + Pf1N1B4_3235 0.74 +1.0 3,622,664 + Pf1N1B4_3235 0.74 -0.5 3,622,664 + Pf1N1B4_3235 0.74 -2.0 3,622,665 - Pf1N1B4_3235 0.75 +1.9 3,622,665 - Pf1N1B4_3235 0.75 +1.3 3,622,665 - Pf1N1B4_3235 0.75 +0.4 3,622,665 - Pf1N1B4_3235 0.75 +1.3 3,622,665 - Pf1N1B4_3235 0.75 -0.7 3,622,665 - Pf1N1B4_3235 0.75 -0.5 3,622,692 - Pf1N1B4_3235 0.78 -0.3 3,622,692 - Pf1N1B4_3235 0.78 -0.0 3,622,703 + Pf1N1B4_3235 0.80 -2.3 3,622,778 + +0.3 3,622,779 - -0.0 3,622,841 + -1.0 3,622,841 + +0.3 3,622,841 + -0.9 3,622,841 + -0.6 3,622,841 + +0.6 3,622,841 + +2.3 3,622,842 - -2.5 3,622,842 - +0.1 3,622,842 - -0.9 3,622,884 + -2.0 3,622,885 - +1.6 3,622,885 - +0.3 3,623,043 + Pf1N1B4_3236 0.35 -1.0 3,623,043 + Pf1N1B4_3236 0.35 +0.3 3,623,043 + Pf1N1B4_3236 0.35 +1.3 3,623,043 + Pf1N1B4_3236 0.35 -0.3 3,623,044 - Pf1N1B4_3236 0.35 +0.3 3,623,044 - Pf1N1B4_3236 0.35 -1.1 3,623,044 - Pf1N1B4_3236 0.35 -1.3
Or see this region's nucleotide sequence