Experiment: D-Galactose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS18165 and HMPREF1058_RS20215 are separated by 131 nucleotides HMPREF1058_RS20215 and HMPREF1058_RS0119310 overlap by 4 nucleotides HMPREF1058_RS0119310 and HMPREF1058_RS18160 are separated by 107 nucleotides HMPREF1058_RS18160 and HMPREF1058_RS18155 are separated by 139 nucleotides
HMPREF1058_RS18165: HMPREF1058_RS18165 - toprim domain-containing protein, at 242,634 to 243,674
_RS18165
HMPREF1058_RS20215: HMPREF1058_RS20215 - hypothetical protein, at 243,806 to 244,141
_RS20215
HMPREF1058_RS0119310: HMPREF1058_RS0119310 - DUF3987 domain-containing protein, at 244,138 to 245,163
_RS0119310
HMPREF1058_RS18160: HMPREF1058_RS18160 - hypothetical protein, at 245,271 to 245,513
_RS18160
HMPREF1058_RS18155: HMPREF1058_RS18155 - helix-turn-helix transcriptional regulator, at 245,653 to 246,315
_RS18155
Position (kb)
244
245
246 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 243.402 kb on - strand, within HMPREF1058_RS18165 at 243.555 kb on - strand, within HMPREF1058_RS18165 at 243.555 kb on - strand, within HMPREF1058_RS18165 at 243.555 kb on - strand, within HMPREF1058_RS18165 at 243.630 kb on + strand at 243.649 kb on - strand at 243.650 kb on + strand at 243.651 kb on - strand at 243.742 kb on + strand at 243.742 kb on + strand at 243.743 kb on - strand at 243.807 kb on + strand at 243.820 kb on + strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.934 kb on + strand, within HMPREF1058_RS20215 at 243.935 kb on - strand, within HMPREF1058_RS20215 at 243.961 kb on - strand, within HMPREF1058_RS20215 at 243.961 kb on - strand, within HMPREF1058_RS20215 at 243.993 kb on - strand, within HMPREF1058_RS20215 at 243.995 kb on + strand, within HMPREF1058_RS20215 at 243.997 kb on + strand, within HMPREF1058_RS20215 at 244.016 kb on - strand, within HMPREF1058_RS20215 at 244.016 kb on - strand, within HMPREF1058_RS20215 at 244.016 kb on - strand, within HMPREF1058_RS20215 at 244.158 kb on + strand at 244.158 kb on + strand at 244.159 kb on - strand at 244.159 kb on - strand at 244.159 kb on - strand at 244.160 kb on + strand at 244.160 kb on + strand at 244.161 kb on - strand at 244.161 kb on - strand at 244.161 kb on - strand at 244.162 kb on + strand at 244.162 kb on + strand at 244.162 kb on + strand at 244.162 kb on + strand at 244.162 kb on + strand at 244.162 kb on + strand at 244.178 kb on + strand at 244.179 kb on - strand at 244.180 kb on + strand at 244.217 kb on - strand at 244.225 kb on - strand at 244.230 kb on + strand at 244.230 kb on + strand at 244.230 kb on + strand at 244.255 kb on - strand, within HMPREF1058_RS0119310 at 244.267 kb on - strand, within HMPREF1058_RS0119310 at 244.288 kb on + strand, within HMPREF1058_RS0119310 at 244.289 kb on - strand, within HMPREF1058_RS0119310 at 244.289 kb on - strand, within HMPREF1058_RS0119310 at 244.315 kb on - strand, within HMPREF1058_RS0119310 at 244.363 kb on + strand, within HMPREF1058_RS0119310 at 244.410 kb on + strand, within HMPREF1058_RS0119310 at 244.516 kb on - strand, within HMPREF1058_RS0119310 at 244.564 kb on + strand, within HMPREF1058_RS0119310 at 244.618 kb on - strand, within HMPREF1058_RS0119310 at 244.620 kb on + strand, within HMPREF1058_RS0119310 at 244.621 kb on - strand, within HMPREF1058_RS0119310 at 244.623 kb on - strand, within HMPREF1058_RS0119310 at 244.664 kb on + strand, within HMPREF1058_RS0119310 at 244.884 kb on + strand, within HMPREF1058_RS0119310 at 244.931 kb on + strand, within HMPREF1058_RS0119310 at 244.931 kb on + strand at 244.988 kb on - strand, within HMPREF1058_RS0119310 at 244.989 kb on + strand, within HMPREF1058_RS0119310 at 244.990 kb on - strand, within HMPREF1058_RS0119310 at 245.011 kb on + strand, within HMPREF1058_RS0119310 at 245.012 kb on - strand, within HMPREF1058_RS0119310 at 245.014 kb on - strand, within HMPREF1058_RS0119310 at 245.039 kb on + strand, within HMPREF1058_RS0119310 at 245.139 kb on + strand at 245.139 kb on + strand at 245.140 kb on - strand at 245.140 kb on - strand at 245.407 kb on + strand, within HMPREF1058_RS18160 at 245.407 kb on + strand, within HMPREF1058_RS18160 at 245.408 kb on - strand, within HMPREF1058_RS18160 at 245.408 kb on - strand, within HMPREF1058_RS18160 at 245.644 kb on + strand at 245.672 kb on + strand at 245.699 kb on + strand at 245.898 kb on + strand, within HMPREF1058_RS18155 at 246.081 kb on + strand, within HMPREF1058_RS18155 at 246.138 kb on + strand, within HMPREF1058_RS18155 at 246.142 kb on + strand, within HMPREF1058_RS18155
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose 20 mM (C) remove 243,402 - HMPREF1058_RS18165 0.74 -1.8 243,555 - HMPREF1058_RS18165 0.88 -0.1 243,555 - HMPREF1058_RS18165 0.88 +0.1 243,555 - HMPREF1058_RS18165 0.88 +0.1 243,630 + +0.2 243,649 - -0.3 243,650 + -0.4 243,651 - -2.5 243,742 + +0.9 243,742 + -0.1 243,743 - -0.6 243,807 + -1.5 243,820 + +1.1 243,821 - +0.9 243,821 - -1.2 243,934 + HMPREF1058_RS20215 0.38 +0.5 243,935 - HMPREF1058_RS20215 0.38 +0.8 243,961 - HMPREF1058_RS20215 0.46 +1.1 243,961 - HMPREF1058_RS20215 0.46 -1.1 243,993 - HMPREF1058_RS20215 0.56 +1.4 243,995 + HMPREF1058_RS20215 0.56 +1.0 243,997 + HMPREF1058_RS20215 0.57 -0.1 244,016 - HMPREF1058_RS20215 0.62 -1.6 244,016 - HMPREF1058_RS20215 0.62 -0.0 244,016 - HMPREF1058_RS20215 0.62 -0.6 244,158 + -1.7 244,158 + +1.0 244,159 - -0.3 244,159 - +0.1 244,159 - +0.3 244,160 + -0.2 244,160 + -2.3 244,161 - -0.9 244,161 - -2.0 244,161 - -1.2 244,162 + +2.4 244,162 + -2.2 244,162 + +1.3 244,162 + -0.4 244,162 + -1.4 244,162 + -1.0 244,178 + -2.5 244,179 - -1.9 244,180 + +0.3 244,217 - -2.3 244,225 - -0.5 244,230 + -0.8 244,230 + +1.1 244,230 + -0.6 244,255 - HMPREF1058_RS0119310 0.11 -0.7 244,267 - HMPREF1058_RS0119310 0.13 +1.0 244,288 + HMPREF1058_RS0119310 0.15 -0.9 244,289 - HMPREF1058_RS0119310 0.15 +0.1 244,289 - HMPREF1058_RS0119310 0.15 +2.2 244,315 - HMPREF1058_RS0119310 0.17 -1.1 244,363 + HMPREF1058_RS0119310 0.22 -1.3 244,410 + HMPREF1058_RS0119310 0.27 +0.6 244,516 - HMPREF1058_RS0119310 0.37 -2.8 244,564 + HMPREF1058_RS0119310 0.42 -1.8 244,618 - HMPREF1058_RS0119310 0.47 -0.0 244,620 + HMPREF1058_RS0119310 0.47 +1.1 244,621 - HMPREF1058_RS0119310 0.47 -1.5 244,623 - HMPREF1058_RS0119310 0.47 -1.6 244,664 + HMPREF1058_RS0119310 0.51 +0.9 244,884 + HMPREF1058_RS0119310 0.73 +2.0 244,931 + HMPREF1058_RS0119310 0.77 +0.6 244,931 + -0.0 244,988 - HMPREF1058_RS0119310 0.83 -1.0 244,989 + HMPREF1058_RS0119310 0.83 -0.6 244,990 - HMPREF1058_RS0119310 0.83 -0.8 245,011 + HMPREF1058_RS0119310 0.85 -2.2 245,012 - HMPREF1058_RS0119310 0.85 -0.7 245,014 - HMPREF1058_RS0119310 0.85 +0.5 245,039 + HMPREF1058_RS0119310 0.88 -0.8 245,139 + -2.5 245,139 + -1.5 245,140 - -0.6 245,140 - +0.8 245,407 + HMPREF1058_RS18160 0.56 -1.0 245,407 + HMPREF1058_RS18160 0.56 +0.4 245,408 - HMPREF1058_RS18160 0.56 +0.8 245,408 - HMPREF1058_RS18160 0.56 +1.6 245,644 + +0.7 245,672 + +1.1 245,699 + -1.3 245,898 + HMPREF1058_RS18155 0.37 +0.6 246,081 + HMPREF1058_RS18155 0.65 -0.7 246,138 + HMPREF1058_RS18155 0.73 +2.8 246,142 + HMPREF1058_RS18155 0.74 +0.3
Or see this region's nucleotide sequence