Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25450 and CA265_RS25455 are separated by 92 nucleotides CA265_RS25455 and CA265_RS25460 are separated by 162 nucleotides
CA265_RS25450: CA265_RS25450 - iron-sulfur cluster assembly accessory protein, at 6,178,827 to 6,179,180
_RS25450
CA265_RS25455: CA265_RS25455 - hypothetical protein, at 6,179,273 to 6,179,803
_RS25455
CA265_RS25460: CA265_RS25460 - methylmalonyl-CoA mutase, at 6,179,966 to 6,181,513
_RS25460
Position (kb)
6179
6180
6181
6182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6179.065 kb on - strand, within CA265_RS25450 at 6179.111 kb on + strand, within CA265_RS25450 at 6179.111 kb on + strand, within CA265_RS25450 at 6179.213 kb on + strand at 6179.272 kb on + strand at 6179.292 kb on + strand at 6179.299 kb on + strand at 6179.299 kb on + strand at 6179.301 kb on + strand at 6179.301 kb on + strand at 6179.355 kb on + strand, within CA265_RS25455 at 6179.356 kb on - strand, within CA265_RS25455 at 6179.385 kb on + strand, within CA265_RS25455 at 6179.385 kb on + strand, within CA265_RS25455 at 6179.422 kb on + strand, within CA265_RS25455 at 6179.433 kb on + strand, within CA265_RS25455 at 6179.434 kb on - strand, within CA265_RS25455 at 6179.464 kb on - strand, within CA265_RS25455 at 6179.489 kb on - strand, within CA265_RS25455 at 6179.489 kb on - strand, within CA265_RS25455 at 6179.535 kb on + strand, within CA265_RS25455 at 6179.545 kb on + strand, within CA265_RS25455 at 6179.582 kb on - strand, within CA265_RS25455 at 6179.601 kb on + strand, within CA265_RS25455 at 6179.645 kb on - strand, within CA265_RS25455 at 6179.660 kb on - strand, within CA265_RS25455 at 6179.670 kb on + strand, within CA265_RS25455 at 6179.773 kb on + strand at 6179.791 kb on + strand at 6179.792 kb on - strand at 6179.792 kb on - strand at 6179.891 kb on + strand at 6179.941 kb on + strand at 6179.942 kb on - strand at 6179.958 kb on - strand at 6179.958 kb on - strand at 6179.962 kb on + strand at 6180.085 kb on + strand at 6180.086 kb on - strand at 6180.089 kb on + strand at 6180.090 kb on - strand at 6180.119 kb on + strand at 6180.119 kb on + strand at 6180.165 kb on - strand, within CA265_RS25460 at 6180.165 kb on - strand, within CA265_RS25460 at 6180.169 kb on - strand, within CA265_RS25460 at 6180.266 kb on - strand, within CA265_RS25460 at 6180.320 kb on - strand, within CA265_RS25460 at 6180.420 kb on + strand, within CA265_RS25460 at 6180.482 kb on + strand, within CA265_RS25460 at 6180.484 kb on + strand, within CA265_RS25460 at 6180.586 kb on + strand, within CA265_RS25460 at 6180.686 kb on - strand, within CA265_RS25460 at 6180.731 kb on - strand, within CA265_RS25460 at 6180.788 kb on - strand, within CA265_RS25460 at 6180.888 kb on + strand, within CA265_RS25460 at 6180.946 kb on + strand, within CA265_RS25460 at 6180.947 kb on - strand, within CA265_RS25460 at 6181.006 kb on - strand, within CA265_RS25460 at 6181.054 kb on - strand, within CA265_RS25460 at 6181.054 kb on - strand, within CA265_RS25460 at 6181.175 kb on + strand, within CA265_RS25460 at 6181.205 kb on + strand, within CA265_RS25460 at 6181.258 kb on + strand, within CA265_RS25460 at 6181.258 kb on + strand, within CA265_RS25460 at 6181.258 kb on + strand, within CA265_RS25460 at 6181.259 kb on - strand, within CA265_RS25460 at 6181.300 kb on + strand, within CA265_RS25460 at 6181.420 kb on + strand at 6181.496 kb on - strand at 6181.508 kb on + strand at 6181.603 kb on - strand at 6181.669 kb on - strand at 6181.706 kb on - strand at 6181.706 kb on - strand at 6181.725 kb on + strand at 6181.726 kb on - strand at 6181.784 kb on - strand at 6181.784 kb on - strand at 6181.784 kb on - strand at 6181.843 kb on - strand at 6181.855 kb on + strand at 6181.926 kb on + strand at 6181.926 kb on + strand at 6181.942 kb on - strand at 6182.032 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 6,179,065 - CA265_RS25450 0.67 -1.9 6,179,111 + CA265_RS25450 0.80 -2.3 6,179,111 + CA265_RS25450 0.80 -0.9 6,179,213 + +0.2 6,179,272 + +1.7 6,179,292 + -0.4 6,179,299 + -1.8 6,179,299 + +0.2 6,179,301 + -2.5 6,179,301 + +0.2 6,179,355 + CA265_RS25455 0.15 -0.6 6,179,356 - CA265_RS25455 0.16 -1.0 6,179,385 + CA265_RS25455 0.21 -0.2 6,179,385 + CA265_RS25455 0.21 -0.1 6,179,422 + CA265_RS25455 0.28 +1.2 6,179,433 + CA265_RS25455 0.30 -2.1 6,179,434 - CA265_RS25455 0.30 -2.1 6,179,464 - CA265_RS25455 0.36 -1.6 6,179,489 - CA265_RS25455 0.41 -1.6 6,179,489 - CA265_RS25455 0.41 +3.0 6,179,535 + CA265_RS25455 0.49 -2.2 6,179,545 + CA265_RS25455 0.51 -0.2 6,179,582 - CA265_RS25455 0.58 +0.4 6,179,601 + CA265_RS25455 0.62 +0.1 6,179,645 - CA265_RS25455 0.70 -1.9 6,179,660 - CA265_RS25455 0.73 +0.6 6,179,670 + CA265_RS25455 0.75 -1.1 6,179,773 + -0.1 6,179,791 + +1.7 6,179,792 - +0.0 6,179,792 - -0.1 6,179,891 + +0.8 6,179,941 + -1.6 6,179,942 - -1.2 6,179,958 - -1.6 6,179,958 - -2.9 6,179,962 + +0.8 6,180,085 + -2.2 6,180,086 - +1.1 6,180,089 + +0.7 6,180,090 - -0.9 6,180,119 + -0.2 6,180,119 + -1.5 6,180,165 - CA265_RS25460 0.13 +0.4 6,180,165 - CA265_RS25460 0.13 +2.8 6,180,169 - CA265_RS25460 0.13 -1.3 6,180,266 - CA265_RS25460 0.19 +0.3 6,180,320 - CA265_RS25460 0.23 +1.1 6,180,420 + CA265_RS25460 0.29 +0.6 6,180,482 + CA265_RS25460 0.33 -2.3 6,180,484 + CA265_RS25460 0.33 +0.7 6,180,586 + CA265_RS25460 0.40 +1.5 6,180,686 - CA265_RS25460 0.47 -0.2 6,180,731 - CA265_RS25460 0.49 -0.3 6,180,788 - CA265_RS25460 0.53 +0.1 6,180,888 + CA265_RS25460 0.60 -2.0 6,180,946 + CA265_RS25460 0.63 -2.1 6,180,947 - CA265_RS25460 0.63 +0.2 6,181,006 - CA265_RS25460 0.67 +0.2 6,181,054 - CA265_RS25460 0.70 +0.1 6,181,054 - CA265_RS25460 0.70 +0.4 6,181,175 + CA265_RS25460 0.78 +0.1 6,181,205 + CA265_RS25460 0.80 -0.1 6,181,258 + CA265_RS25460 0.83 +0.2 6,181,258 + CA265_RS25460 0.83 -0.7 6,181,258 + CA265_RS25460 0.83 +0.7 6,181,259 - CA265_RS25460 0.84 -2.0 6,181,300 + CA265_RS25460 0.86 -1.4 6,181,420 + -1.5 6,181,496 - +0.8 6,181,508 + -2.1 6,181,603 - -0.2 6,181,669 - +0.6 6,181,706 - +0.7 6,181,706 - +0.4 6,181,725 + -2.6 6,181,726 - -0.7 6,181,784 - -2.9 6,181,784 - +0.1 6,181,784 - -1.3 6,181,843 - +0.3 6,181,855 + +0.1 6,181,926 + -1.0 6,181,926 + -2.4 6,181,942 - +0.4 6,182,032 + +0.9
Or see this region's nucleotide sequence