Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25210 and CA265_RS25215 are separated by 217 nucleotides CA265_RS25215 and CA265_RS25220 are separated by 108 nucleotides
CA265_RS25210: CA265_RS25210 - lipid-binding protein, at 6,136,391 to 6,136,978
_RS25210
CA265_RS25215: CA265_RS25215 - MBL fold hydrolase, at 6,137,196 to 6,137,966
_RS25215
CA265_RS25220: CA265_RS25220 - sulfatase, at 6,138,075 to 6,139,400
_RS25220
Position (kb)
6137
6138 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6136.268 kb on - strand at 6136.284 kb on + strand at 6136.298 kb on - strand at 6136.307 kb on + strand at 6136.328 kb on + strand at 6136.329 kb on - strand at 6136.329 kb on - strand at 6136.329 kb on - strand at 6136.422 kb on + strand at 6136.435 kb on - strand at 6136.475 kb on + strand, within CA265_RS25210 at 6136.475 kb on + strand, within CA265_RS25210 at 6136.476 kb on - strand, within CA265_RS25210 at 6136.476 kb on - strand, within CA265_RS25210 at 6136.476 kb on - strand, within CA265_RS25210 at 6136.482 kb on + strand, within CA265_RS25210 at 6136.483 kb on - strand, within CA265_RS25210 at 6136.525 kb on - strand, within CA265_RS25210 at 6136.564 kb on + strand, within CA265_RS25210 at 6136.609 kb on + strand, within CA265_RS25210 at 6136.609 kb on + strand, within CA265_RS25210 at 6136.665 kb on + strand, within CA265_RS25210 at 6136.724 kb on - strand, within CA265_RS25210 at 6136.767 kb on + strand, within CA265_RS25210 at 6136.784 kb on - strand, within CA265_RS25210 at 6136.786 kb on + strand, within CA265_RS25210 at 6136.787 kb on - strand, within CA265_RS25210 at 6136.812 kb on + strand, within CA265_RS25210 at 6136.877 kb on + strand, within CA265_RS25210 at 6136.878 kb on - strand, within CA265_RS25210 at 6137.108 kb on + strand at 6137.135 kb on - strand at 6137.148 kb on + strand at 6137.148 kb on + strand at 6137.197 kb on + strand at 6137.198 kb on - strand at 6137.229 kb on + strand at 6137.230 kb on - strand at 6137.230 kb on - strand at 6137.230 kb on - strand at 6137.509 kb on - strand, within CA265_RS25215 at 6137.561 kb on - strand, within CA265_RS25215 at 6137.561 kb on - strand, within CA265_RS25215 at 6137.610 kb on + strand, within CA265_RS25215 at 6137.706 kb on + strand, within CA265_RS25215 at 6137.706 kb on + strand, within CA265_RS25215 at 6137.707 kb on - strand, within CA265_RS25215 at 6137.715 kb on + strand, within CA265_RS25215 at 6137.723 kb on + strand, within CA265_RS25215 at 6137.886 kb on + strand, within CA265_RS25215 at 6137.932 kb on + strand at 6137.933 kb on - strand at 6137.933 kb on - strand at 6137.937 kb on - strand at 6137.986 kb on - strand at 6137.994 kb on - strand at 6138.011 kb on + strand at 6138.054 kb on + strand at 6138.120 kb on - strand at 6138.156 kb on - strand at 6138.161 kb on - strand at 6138.275 kb on + strand, within CA265_RS25220 at 6138.275 kb on + strand, within CA265_RS25220 at 6138.291 kb on - strand, within CA265_RS25220 at 6138.314 kb on + strand, within CA265_RS25220 at 6138.324 kb on + strand, within CA265_RS25220 at 6138.486 kb on - strand, within CA265_RS25220 at 6138.519 kb on + strand, within CA265_RS25220 at 6138.520 kb on - strand, within CA265_RS25220 at 6138.520 kb on - strand, within CA265_RS25220 at 6138.543 kb on + strand, within CA265_RS25220 at 6138.581 kb on + strand, within CA265_RS25220 at 6138.584 kb on + strand, within CA265_RS25220 at 6138.597 kb on + strand, within CA265_RS25220 at 6138.597 kb on + strand, within CA265_RS25220 at 6138.598 kb on - strand, within CA265_RS25220 at 6138.611 kb on + strand, within CA265_RS25220 at 6138.615 kb on - strand, within CA265_RS25220 at 6138.631 kb on - strand, within CA265_RS25220 at 6138.656 kb on + strand, within CA265_RS25220 at 6138.707 kb on + strand, within CA265_RS25220 at 6138.708 kb on - strand, within CA265_RS25220 at 6138.912 kb on + strand, within CA265_RS25220 at 6138.912 kb on + strand, within CA265_RS25220 at 6138.913 kb on - strand, within CA265_RS25220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 6,136,268 - -0.1 6,136,284 + -0.8 6,136,298 - -0.3 6,136,307 + +1.1 6,136,328 + -0.4 6,136,329 - +1.1 6,136,329 - +2.0 6,136,329 - -1.2 6,136,422 + +0.5 6,136,435 - +0.3 6,136,475 + CA265_RS25210 0.14 +0.3 6,136,475 + CA265_RS25210 0.14 +0.1 6,136,476 - CA265_RS25210 0.14 +0.6 6,136,476 - CA265_RS25210 0.14 +0.8 6,136,476 - CA265_RS25210 0.14 -0.6 6,136,482 + CA265_RS25210 0.15 +1.0 6,136,483 - CA265_RS25210 0.16 -0.4 6,136,525 - CA265_RS25210 0.23 +1.8 6,136,564 + CA265_RS25210 0.29 -1.8 6,136,609 + CA265_RS25210 0.37 -1.5 6,136,609 + CA265_RS25210 0.37 +2.4 6,136,665 + CA265_RS25210 0.47 +0.3 6,136,724 - CA265_RS25210 0.57 +2.6 6,136,767 + CA265_RS25210 0.64 -3.1 6,136,784 - CA265_RS25210 0.67 -0.9 6,136,786 + CA265_RS25210 0.67 +0.1 6,136,787 - CA265_RS25210 0.67 +0.8 6,136,812 + CA265_RS25210 0.72 -0.2 6,136,877 + CA265_RS25210 0.83 +0.7 6,136,878 - CA265_RS25210 0.83 -0.1 6,137,108 + +1.6 6,137,135 - -0.8 6,137,148 + -0.0 6,137,148 + +0.1 6,137,197 + -0.8 6,137,198 - -2.1 6,137,229 + +0.2 6,137,230 - +0.4 6,137,230 - +0.3 6,137,230 - -0.5 6,137,509 - CA265_RS25215 0.41 -1.5 6,137,561 - CA265_RS25215 0.47 -0.0 6,137,561 - CA265_RS25215 0.47 -1.7 6,137,610 + CA265_RS25215 0.54 -0.6 6,137,706 + CA265_RS25215 0.66 +1.9 6,137,706 + CA265_RS25215 0.66 -2.6 6,137,707 - CA265_RS25215 0.66 -2.4 6,137,715 + CA265_RS25215 0.67 -0.8 6,137,723 + CA265_RS25215 0.68 -1.9 6,137,886 + CA265_RS25215 0.89 -0.1 6,137,932 + -1.7 6,137,933 - +0.7 6,137,933 - -0.1 6,137,937 - -3.5 6,137,986 - -1.2 6,137,994 - -0.2 6,138,011 + -1.2 6,138,054 + +0.8 6,138,120 - +0.1 6,138,156 - -1.4 6,138,161 - +0.8 6,138,275 + CA265_RS25220 0.15 -1.0 6,138,275 + CA265_RS25220 0.15 +1.2 6,138,291 - CA265_RS25220 0.16 -1.3 6,138,314 + CA265_RS25220 0.18 +0.1 6,138,324 + CA265_RS25220 0.19 -1.1 6,138,486 - CA265_RS25220 0.31 -1.2 6,138,519 + CA265_RS25220 0.33 -0.0 6,138,520 - CA265_RS25220 0.34 +1.7 6,138,520 - CA265_RS25220 0.34 +0.4 6,138,543 + CA265_RS25220 0.35 +0.9 6,138,581 + CA265_RS25220 0.38 -1.3 6,138,584 + CA265_RS25220 0.38 +0.4 6,138,597 + CA265_RS25220 0.39 +0.1 6,138,597 + CA265_RS25220 0.39 +0.8 6,138,598 - CA265_RS25220 0.39 -1.8 6,138,611 + CA265_RS25220 0.40 +0.1 6,138,615 - CA265_RS25220 0.41 +0.8 6,138,631 - CA265_RS25220 0.42 +0.1 6,138,656 + CA265_RS25220 0.44 +0.2 6,138,707 + CA265_RS25220 0.48 -0.2 6,138,708 - CA265_RS25220 0.48 -1.1 6,138,912 + CA265_RS25220 0.63 +1.7 6,138,912 + CA265_RS25220 0.63 -0.0 6,138,913 - CA265_RS25220 0.63 -0.2
Or see this region's nucleotide sequence