Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23500 and CA265_RS23505 are separated by 3 nucleotides CA265_RS23505 and CA265_RS23510 are separated by 19 nucleotides CA265_RS23510 and CA265_RS23515 are separated by 6 nucleotides CA265_RS23515 and CA265_RS23520 are separated by 3 nucleotides
CA265_RS23500: CA265_RS23500 - biopolymer transporter ExbD, at 5,748,532 to 5,749,146
_RS23500
CA265_RS23505: CA265_RS23505 - biopolymer transporter ExbD, at 5,749,150 to 5,749,656
_RS23505
CA265_RS23510: CA265_RS23510 - energy transducer TonB, at 5,749,676 to 5,750,494
_RS23510
CA265_RS23515: CA265_RS23515 - hypothetical protein, at 5,750,501 to 5,750,728
_RS23515
CA265_RS23520: CA265_RS23520 - phosphate ABC transporter substrate-binding protein, at 5,750,732 to 5,751,634
_RS23520
Position (kb) 5749
5750
5751 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5749.160 kb on + strand at 5749.197 kb on + strand at 5749.217 kb on + strand, within CA265_RS23505 at 5749.218 kb on - strand, within CA265_RS23505 at 5749.260 kb on - strand, within CA265_RS23505 at 5749.387 kb on + strand, within CA265_RS23505 at 5749.449 kb on + strand, within CA265_RS23505 at 5749.456 kb on - strand, within CA265_RS23505 at 5749.530 kb on + strand, within CA265_RS23505 at 5749.551 kb on + strand, within CA265_RS23505 at 5749.617 kb on + strand at 5749.661 kb on + strand at 5749.671 kb on + strand at 5749.690 kb on + strand at 5749.762 kb on + strand at 5749.795 kb on - strand, within CA265_RS23510 at 5749.798 kb on + strand, within CA265_RS23510 at 5749.818 kb on + strand, within CA265_RS23510 at 5749.933 kb on + strand, within CA265_RS23510 at 5750.231 kb on - strand, within CA265_RS23510 at 5750.247 kb on + strand, within CA265_RS23510 at 5750.252 kb on - strand, within CA265_RS23510 at 5750.260 kb on + strand, within CA265_RS23510 at 5750.299 kb on + strand, within CA265_RS23510 at 5750.482 kb on + strand at 5750.492 kb on + strand at 5750.492 kb on + strand at 5750.498 kb on + strand at 5750.515 kb on + strand at 5750.515 kb on + strand at 5750.583 kb on - strand, within CA265_RS23515 at 5750.605 kb on + strand, within CA265_RS23515 at 5750.684 kb on - strand, within CA265_RS23515 at 5750.684 kb on - strand, within CA265_RS23515 at 5750.698 kb on - strand, within CA265_RS23515 at 5750.706 kb on + strand at 5750.828 kb on - strand, within CA265_RS23520 at 5750.833 kb on + strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.949 kb on + strand, within CA265_RS23520 at 5750.997 kb on + strand, within CA265_RS23520 at 5751.037 kb on + strand, within CA265_RS23520 at 5751.067 kb on - strand, within CA265_RS23520 at 5751.082 kb on - strand, within CA265_RS23520 at 5751.109 kb on + strand, within CA265_RS23520 at 5751.110 kb on - strand, within CA265_RS23520 at 5751.136 kb on + strand, within CA265_RS23520 at 5751.137 kb on - strand, within CA265_RS23520 at 5751.138 kb on + strand, within CA265_RS23520 at 5751.139 kb on - strand, within CA265_RS23520 at 5751.141 kb on + strand, within CA265_RS23520 at 5751.185 kb on + strand, within CA265_RS23520 at 5751.313 kb on + strand, within CA265_RS23520 at 5751.363 kb on - strand, within CA265_RS23520 at 5751.411 kb on + strand, within CA265_RS23520 at 5751.412 kb on - strand, within CA265_RS23520 at 5751.426 kb on - strand, within CA265_RS23520 at 5751.426 kb on - strand at 5751.451 kb on - strand, within CA265_RS23520 at 5751.483 kb on + strand, within CA265_RS23520 at 5751.484 kb on - strand, within CA265_RS23520 at 5751.484 kb on - strand, within CA265_RS23520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,749,160 + -0.8 5,749,197 + +3.2 5,749,217 + CA265_RS23505 0.13 -1.7 5,749,218 - CA265_RS23505 0.13 -0.4 5,749,260 - CA265_RS23505 0.22 -0.2 5,749,387 + CA265_RS23505 0.47 +0.4 5,749,449 + CA265_RS23505 0.59 -1.6 5,749,456 - CA265_RS23505 0.60 +0.1 5,749,530 + CA265_RS23505 0.75 -0.0 5,749,551 + CA265_RS23505 0.79 +1.5 5,749,617 + -0.2 5,749,661 + +0.6 5,749,671 + +2.4 5,749,690 + -0.2 5,749,762 + +0.8 5,749,795 - CA265_RS23510 0.15 +0.4 5,749,798 + CA265_RS23510 0.15 +1.3 5,749,818 + CA265_RS23510 0.17 +0.5 5,749,933 + CA265_RS23510 0.31 +0.5 5,750,231 - CA265_RS23510 0.68 +1.8 5,750,247 + CA265_RS23510 0.70 +1.3 5,750,252 - CA265_RS23510 0.70 +0.5 5,750,260 + CA265_RS23510 0.71 -0.5 5,750,299 + CA265_RS23510 0.76 +1.5 5,750,482 + -0.8 5,750,492 + +1.1 5,750,492 + -1.4 5,750,498 + +0.0 5,750,515 + -1.7 5,750,515 + +0.8 5,750,583 - CA265_RS23515 0.36 -0.8 5,750,605 + CA265_RS23515 0.46 +0.1 5,750,684 - CA265_RS23515 0.80 +0.7 5,750,684 - CA265_RS23515 0.80 +1.0 5,750,698 - CA265_RS23515 0.86 +2.3 5,750,706 + +0.4 5,750,828 - CA265_RS23520 0.11 +2.1 5,750,833 + CA265_RS23520 0.11 +0.0 5,750,834 - CA265_RS23520 0.11 -0.7 5,750,834 - CA265_RS23520 0.11 +0.3 5,750,834 - CA265_RS23520 0.11 +0.7 5,750,834 - CA265_RS23520 0.11 -2.5 5,750,949 + CA265_RS23520 0.24 -0.5 5,750,997 + CA265_RS23520 0.29 -0.2 5,751,037 + CA265_RS23520 0.34 -1.4 5,751,067 - CA265_RS23520 0.37 -0.1 5,751,082 - CA265_RS23520 0.39 -2.1 5,751,109 + CA265_RS23520 0.42 -0.9 5,751,110 - CA265_RS23520 0.42 +0.7 5,751,136 + CA265_RS23520 0.45 +0.8 5,751,137 - CA265_RS23520 0.45 +0.1 5,751,138 + CA265_RS23520 0.45 -0.3 5,751,139 - CA265_RS23520 0.45 +2.1 5,751,141 + CA265_RS23520 0.45 +0.5 5,751,185 + CA265_RS23520 0.50 -1.9 5,751,313 + CA265_RS23520 0.64 +2.0 5,751,363 - CA265_RS23520 0.70 -3.0 5,751,411 + CA265_RS23520 0.75 -0.5 5,751,412 - CA265_RS23520 0.75 +0.8 5,751,426 - CA265_RS23520 0.77 +0.0 5,751,426 - +0.8 5,751,451 - CA265_RS23520 0.80 +0.5 5,751,483 + CA265_RS23520 0.83 -2.2 5,751,484 - CA265_RS23520 0.83 -1.0 5,751,484 - CA265_RS23520 0.83 -0.5
Or see this region's nucleotide sequence