Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22225 and CA265_RS22230 are separated by 1 nucleotides CA265_RS22230 and CA265_RS22235 are separated by 12 nucleotides CA265_RS22235 and CA265_RS22240 are separated by 1 nucleotides CA265_RS22240 and CA265_RS22245 are separated by 3 nucleotides
CA265_RS22225: CA265_RS22225 - hypothetical protein, at 5,460,714 to 5,461,265
_RS22225
CA265_RS22230: CA265_RS22230 - hypothetical protein, at 5,461,267 to 5,461,854
_RS22230
CA265_RS22235: CA265_RS22235 - type IV secretion protein Rhs, at 5,461,867 to 5,463,666
_RS22235
CA265_RS22240: CA265_RS22240 - hypothetical protein, at 5,463,668 to 5,464,471
_RS22240
CA265_RS22245: CA265_RS22245 - hypothetical protein, at 5,464,475 to 5,466,127
_RS22245
Position (kb) 5461
5462
5463
5464 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5460.897 kb on - strand, within CA265_RS22225 at 5460.986 kb on + strand, within CA265_RS22225 at 5461.086 kb on + strand, within CA265_RS22225 at 5461.087 kb on - strand, within CA265_RS22225 at 5461.224 kb on - strand at 5461.224 kb on - strand at 5461.224 kb on - strand at 5461.232 kb on + strand at 5461.233 kb on - strand at 5461.323 kb on + strand at 5461.369 kb on + strand, within CA265_RS22230 at 5461.374 kb on + strand, within CA265_RS22230 at 5461.375 kb on - strand, within CA265_RS22230 at 5461.375 kb on - strand, within CA265_RS22230 at 5461.380 kb on + strand, within CA265_RS22230 at 5461.425 kb on + strand, within CA265_RS22230 at 5461.426 kb on - strand, within CA265_RS22230 at 5461.426 kb on - strand, within CA265_RS22230 at 5461.428 kb on + strand, within CA265_RS22230 at 5461.429 kb on - strand, within CA265_RS22230 at 5461.540 kb on - strand, within CA265_RS22230 at 5461.555 kb on - strand, within CA265_RS22230 at 5461.620 kb on + strand, within CA265_RS22230 at 5461.620 kb on + strand, within CA265_RS22230 at 5461.738 kb on - strand, within CA265_RS22230 at 5461.738 kb on - strand, within CA265_RS22230 at 5461.788 kb on + strand, within CA265_RS22230 at 5461.789 kb on - strand, within CA265_RS22230 at 5461.789 kb on - strand, within CA265_RS22230 at 5461.894 kb on - strand at 5461.894 kb on - strand at 5461.898 kb on + strand at 5461.899 kb on - strand at 5461.933 kb on - strand at 5461.933 kb on - strand at 5461.937 kb on + strand at 5461.951 kb on - strand at 5461.964 kb on - strand at 5461.981 kb on - strand at 5462.164 kb on + strand, within CA265_RS22235 at 5462.279 kb on + strand, within CA265_RS22235 at 5462.279 kb on + strand, within CA265_RS22235 at 5462.307 kb on + strand, within CA265_RS22235 at 5462.307 kb on + strand, within CA265_RS22235 at 5462.314 kb on + strand, within CA265_RS22235 at 5462.315 kb on - strand at 5462.315 kb on - strand, within CA265_RS22235 at 5462.315 kb on - strand, within CA265_RS22235 at 5462.386 kb on + strand, within CA265_RS22235 at 5462.477 kb on - strand, within CA265_RS22235 at 5462.477 kb on - strand, within CA265_RS22235 at 5462.518 kb on + strand, within CA265_RS22235 at 5462.597 kb on + strand at 5462.598 kb on - strand, within CA265_RS22235 at 5462.617 kb on + strand, within CA265_RS22235 at 5462.622 kb on + strand, within CA265_RS22235 at 5462.654 kb on + strand, within CA265_RS22235 at 5462.655 kb on - strand, within CA265_RS22235 at 5462.707 kb on - strand, within CA265_RS22235 at 5462.707 kb on - strand, within CA265_RS22235 at 5462.726 kb on + strand, within CA265_RS22235 at 5462.784 kb on + strand, within CA265_RS22235 at 5462.808 kb on + strand, within CA265_RS22235 at 5462.952 kb on + strand, within CA265_RS22235 at 5462.969 kb on + strand, within CA265_RS22235 at 5463.030 kb on + strand, within CA265_RS22235 at 5463.066 kb on + strand, within CA265_RS22235 at 5463.168 kb on + strand, within CA265_RS22235 at 5463.169 kb on - strand, within CA265_RS22235 at 5463.217 kb on - strand, within CA265_RS22235 at 5463.252 kb on - strand, within CA265_RS22235 at 5463.270 kb on + strand, within CA265_RS22235 at 5463.270 kb on + strand, within CA265_RS22235 at 5463.271 kb on - strand, within CA265_RS22235 at 5463.271 kb on - strand, within CA265_RS22235 at 5463.271 kb on - strand, within CA265_RS22235 at 5463.271 kb on - strand, within CA265_RS22235 at 5463.276 kb on - strand, within CA265_RS22235 at 5463.305 kb on + strand, within CA265_RS22235 at 5463.331 kb on - strand, within CA265_RS22235 at 5463.354 kb on + strand, within CA265_RS22235 at 5463.375 kb on + strand, within CA265_RS22235 at 5463.375 kb on + strand, within CA265_RS22235 at 5463.376 kb on - strand, within CA265_RS22235 at 5463.379 kb on - strand, within CA265_RS22235 at 5463.430 kb on - strand, within CA265_RS22235 at 5463.430 kb on - strand, within CA265_RS22235 at 5463.439 kb on - strand, within CA265_RS22235 at 5463.508 kb on - strand at 5463.540 kb on - strand at 5463.543 kb on + strand at 5463.543 kb on + strand at 5463.613 kb on - strand at 5463.625 kb on - strand at 5463.625 kb on - strand at 5463.753 kb on + strand, within CA265_RS22240 at 5463.761 kb on + strand, within CA265_RS22240 at 5463.762 kb on - strand, within CA265_RS22240 at 5463.765 kb on + strand, within CA265_RS22240 at 5463.765 kb on + strand, within CA265_RS22240 at 5463.766 kb on - strand, within CA265_RS22240 at 5463.768 kb on + strand, within CA265_RS22240 at 5463.819 kb on + strand, within CA265_RS22240 at 5463.820 kb on - strand, within CA265_RS22240 at 5463.882 kb on - strand, within CA265_RS22240 at 5463.900 kb on - strand, within CA265_RS22240 at 5463.905 kb on + strand, within CA265_RS22240 at 5463.927 kb on + strand, within CA265_RS22240 at 5463.931 kb on + strand, within CA265_RS22240 at 5463.932 kb on - strand, within CA265_RS22240 at 5463.932 kb on - strand, within CA265_RS22240 at 5463.957 kb on + strand, within CA265_RS22240 at 5463.957 kb on + strand at 5463.958 kb on - strand, within CA265_RS22240 at 5463.989 kb on - strand, within CA265_RS22240 at 5464.022 kb on - strand, within CA265_RS22240 at 5464.122 kb on + strand, within CA265_RS22240 at 5464.136 kb on + strand, within CA265_RS22240 at 5464.250 kb on + strand, within CA265_RS22240 at 5464.270 kb on + strand, within CA265_RS22240 at 5464.270 kb on + strand, within CA265_RS22240 at 5464.276 kb on - strand, within CA265_RS22240 at 5464.294 kb on - strand, within CA265_RS22240 at 5464.294 kb on - strand, within CA265_RS22240 at 5464.297 kb on + strand, within CA265_RS22240 at 5464.298 kb on - strand, within CA265_RS22240 at 5464.340 kb on - strand, within CA265_RS22240 at 5464.343 kb on - strand, within CA265_RS22240 at 5464.371 kb on - strand, within CA265_RS22240 at 5464.373 kb on - strand, within CA265_RS22240 at 5464.434 kb on - strand at 5464.446 kb on + strand at 5464.601 kb on - strand at 5464.648 kb on + strand, within CA265_RS22245 at 5464.658 kb on - strand, within CA265_RS22245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,460,897 - CA265_RS22225 0.33 +0.5 5,460,986 + CA265_RS22225 0.49 +1.4 5,461,086 + CA265_RS22225 0.67 -0.6 5,461,087 - CA265_RS22225 0.68 +0.8 5,461,224 - -1.5 5,461,224 - -0.8 5,461,224 - -0.5 5,461,232 + +0.1 5,461,233 - -0.8 5,461,323 + +0.6 5,461,369 + CA265_RS22230 0.17 -0.8 5,461,374 + CA265_RS22230 0.18 -0.5 5,461,375 - CA265_RS22230 0.18 -0.5 5,461,375 - CA265_RS22230 0.18 -0.1 5,461,380 + CA265_RS22230 0.19 +1.6 5,461,425 + CA265_RS22230 0.27 -2.2 5,461,426 - CA265_RS22230 0.27 +0.4 5,461,426 - CA265_RS22230 0.27 -0.3 5,461,428 + CA265_RS22230 0.27 +0.5 5,461,429 - CA265_RS22230 0.28 +0.0 5,461,540 - CA265_RS22230 0.46 -1.8 5,461,555 - CA265_RS22230 0.49 -2.2 5,461,620 + CA265_RS22230 0.60 -1.2 5,461,620 + CA265_RS22230 0.60 -3.3 5,461,738 - CA265_RS22230 0.80 +0.4 5,461,738 - CA265_RS22230 0.80 -0.3 5,461,788 + CA265_RS22230 0.89 +2.0 5,461,789 - CA265_RS22230 0.89 +0.9 5,461,789 - CA265_RS22230 0.89 -0.1 5,461,894 - -1.6 5,461,894 - -0.2 5,461,898 + -2.5 5,461,899 - +1.3 5,461,933 - +1.5 5,461,933 - +1.0 5,461,937 + -0.8 5,461,951 - +0.6 5,461,964 - +1.4 5,461,981 - +0.8 5,462,164 + CA265_RS22235 0.17 -1.5 5,462,279 + CA265_RS22235 0.23 -0.1 5,462,279 + CA265_RS22235 0.23 -1.3 5,462,307 + CA265_RS22235 0.24 +0.4 5,462,307 + CA265_RS22235 0.24 -1.6 5,462,314 + CA265_RS22235 0.25 +0.1 5,462,315 - +3.0 5,462,315 - CA265_RS22235 0.25 +1.7 5,462,315 - CA265_RS22235 0.25 +0.4 5,462,386 + CA265_RS22235 0.29 +0.2 5,462,477 - CA265_RS22235 0.34 -0.4 5,462,477 - CA265_RS22235 0.34 +0.6 5,462,518 + CA265_RS22235 0.36 +2.9 5,462,597 + +0.6 5,462,598 - CA265_RS22235 0.41 +0.1 5,462,617 + CA265_RS22235 0.42 -1.1 5,462,622 + CA265_RS22235 0.42 -0.1 5,462,654 + CA265_RS22235 0.44 +0.3 5,462,655 - CA265_RS22235 0.44 -1.4 5,462,707 - CA265_RS22235 0.47 +0.7 5,462,707 - CA265_RS22235 0.47 -0.8 5,462,726 + CA265_RS22235 0.48 -0.2 5,462,784 + CA265_RS22235 0.51 -0.8 5,462,808 + CA265_RS22235 0.52 -0.5 5,462,952 + CA265_RS22235 0.60 +2.2 5,462,969 + CA265_RS22235 0.61 -1.5 5,463,030 + CA265_RS22235 0.65 +1.2 5,463,066 + CA265_RS22235 0.67 -0.5 5,463,168 + CA265_RS22235 0.72 +0.7 5,463,169 - CA265_RS22235 0.72 -2.2 5,463,217 - CA265_RS22235 0.75 +2.0 5,463,252 - CA265_RS22235 0.77 +1.0 5,463,270 + CA265_RS22235 0.78 -1.8 5,463,270 + CA265_RS22235 0.78 +2.1 5,463,271 - CA265_RS22235 0.78 -1.8 5,463,271 - CA265_RS22235 0.78 -0.7 5,463,271 - CA265_RS22235 0.78 +0.6 5,463,271 - CA265_RS22235 0.78 -1.1 5,463,276 - CA265_RS22235 0.78 +2.0 5,463,305 + CA265_RS22235 0.80 +0.8 5,463,331 - CA265_RS22235 0.81 +0.8 5,463,354 + CA265_RS22235 0.83 -1.5 5,463,375 + CA265_RS22235 0.84 +0.8 5,463,375 + CA265_RS22235 0.84 +1.4 5,463,376 - CA265_RS22235 0.84 +0.8 5,463,379 - CA265_RS22235 0.84 -3.6 5,463,430 - CA265_RS22235 0.87 -0.7 5,463,430 - CA265_RS22235 0.87 +0.6 5,463,439 - CA265_RS22235 0.87 -0.3 5,463,508 - -0.2 5,463,540 - +1.2 5,463,543 + -0.4 5,463,543 + +0.2 5,463,613 - -1.3 5,463,625 - +0.8 5,463,625 - +0.3 5,463,753 + CA265_RS22240 0.11 -0.4 5,463,761 + CA265_RS22240 0.12 +0.6 5,463,762 - CA265_RS22240 0.12 +1.5 5,463,765 + CA265_RS22240 0.12 -0.5 5,463,765 + CA265_RS22240 0.12 -0.5 5,463,766 - CA265_RS22240 0.12 +1.3 5,463,768 + CA265_RS22240 0.12 +0.4 5,463,819 + CA265_RS22240 0.19 -1.7 5,463,820 - CA265_RS22240 0.19 +0.6 5,463,882 - CA265_RS22240 0.27 +0.3 5,463,900 - CA265_RS22240 0.29 +1.3 5,463,905 + CA265_RS22240 0.29 +0.4 5,463,927 + CA265_RS22240 0.32 +0.4 5,463,931 + CA265_RS22240 0.33 +1.6 5,463,932 - CA265_RS22240 0.33 +0.2 5,463,932 - CA265_RS22240 0.33 -1.6 5,463,957 + CA265_RS22240 0.36 -2.6 5,463,957 + +0.6 5,463,958 - CA265_RS22240 0.36 +0.4 5,463,989 - CA265_RS22240 0.40 +0.2 5,464,022 - CA265_RS22240 0.44 +1.3 5,464,122 + CA265_RS22240 0.56 -2.2 5,464,136 + CA265_RS22240 0.58 +0.9 5,464,250 + CA265_RS22240 0.72 +3.1 5,464,270 + CA265_RS22240 0.75 -1.8 5,464,270 + CA265_RS22240 0.75 -0.1 5,464,276 - CA265_RS22240 0.76 +0.4 5,464,294 - CA265_RS22240 0.78 -0.8 5,464,294 - CA265_RS22240 0.78 +0.6 5,464,297 + CA265_RS22240 0.78 -0.4 5,464,298 - CA265_RS22240 0.78 -1.8 5,464,340 - CA265_RS22240 0.84 -0.6 5,464,343 - CA265_RS22240 0.84 +1.5 5,464,371 - CA265_RS22240 0.87 -0.7 5,464,373 - CA265_RS22240 0.88 +0.3 5,464,434 - +1.4 5,464,446 + +0.6 5,464,601 - -0.2 5,464,648 + CA265_RS22245 0.10 +2.6 5,464,658 - CA265_RS22245 0.11 -0.1
Or see this region's nucleotide sequence