Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22070 and CA265_RS22075 are separated by 519 nucleotides CA265_RS22075 and CA265_RS22080 are separated by 127 nucleotides CA265_RS22080 and CA265_RS22085 are separated by 238 nucleotides
CA265_RS22070: CA265_RS22070 - hypothetical protein, at 5,425,465 to 5,425,662
_RS22070
CA265_RS22075: CA265_RS22075 - hypothetical protein, at 5,426,182 to 5,426,601
_RS22075
CA265_RS22080: CA265_RS22080 - hypothetical protein, at 5,426,729 to 5,427,145
_RS22080
CA265_RS22085: CA265_RS22085 - N-acetylgalactosamine-6-sulfatase, at 5,427,384 to 5,428,907
_RS22085
Position (kb)
5426
5427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5425.234 kb on + strand at 5425.235 kb on - strand at 5425.249 kb on - strand at 5425.292 kb on + strand at 5425.299 kb on - strand at 5425.331 kb on + strand at 5425.332 kb on - strand at 5425.494 kb on + strand, within CA265_RS22070 at 5425.496 kb on + strand, within CA265_RS22070 at 5425.505 kb on + strand, within CA265_RS22070 at 5425.546 kb on + strand, within CA265_RS22070 at 5425.549 kb on - strand, within CA265_RS22070 at 5425.560 kb on + strand, within CA265_RS22070 at 5425.631 kb on + strand, within CA265_RS22070 at 5425.631 kb on + strand, within CA265_RS22070 at 5425.633 kb on + strand, within CA265_RS22070 at 5425.633 kb on + strand, within CA265_RS22070 at 5425.661 kb on - strand at 5425.666 kb on + strand at 5425.666 kb on + strand at 5425.691 kb on - strand at 5425.699 kb on + strand at 5425.764 kb on + strand at 5425.785 kb on - strand at 5425.785 kb on - strand at 5425.805 kb on - strand at 5425.891 kb on + strand at 5425.892 kb on - strand at 5425.997 kb on + strand at 5425.998 kb on - strand at 5426.012 kb on + strand at 5426.026 kb on + strand at 5426.032 kb on - strand at 5426.079 kb on + strand at 5426.080 kb on - strand at 5426.108 kb on + strand at 5426.126 kb on - strand at 5426.126 kb on - strand at 5426.126 kb on - strand at 5426.128 kb on - strand at 5426.162 kb on + strand at 5426.193 kb on - strand at 5426.193 kb on - strand at 5426.268 kb on + strand, within CA265_RS22075 at 5426.280 kb on + strand, within CA265_RS22075 at 5426.281 kb on - strand, within CA265_RS22075 at 5426.325 kb on + strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.327 kb on + strand, within CA265_RS22075 at 5426.416 kb on - strand, within CA265_RS22075 at 5426.424 kb on + strand, within CA265_RS22075 at 5426.460 kb on + strand, within CA265_RS22075 at 5426.460 kb on + strand, within CA265_RS22075 at 5426.461 kb on - strand, within CA265_RS22075 at 5426.540 kb on + strand at 5426.608 kb on + strand at 5426.672 kb on + strand at 5426.672 kb on + strand at 5426.677 kb on - strand at 5426.749 kb on - strand at 5426.751 kb on + strand at 5426.751 kb on + strand at 5426.762 kb on - strand at 5426.818 kb on + strand, within CA265_RS22080 at 5426.854 kb on - strand, within CA265_RS22080 at 5426.896 kb on - strand, within CA265_RS22080 at 5426.955 kb on + strand, within CA265_RS22080 at 5427.042 kb on + strand, within CA265_RS22080 at 5427.150 kb on + strand at 5427.258 kb on + strand at 5427.383 kb on + strand at 5427.395 kb on - strand at 5427.422 kb on - strand at 5427.464 kb on + strand at 5427.486 kb on - strand at 5427.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,425,234 + -1.5 5,425,235 - -1.0 5,425,249 - -1.0 5,425,292 + +0.7 5,425,299 - -1.5 5,425,331 + +0.5 5,425,332 - +0.2 5,425,494 + CA265_RS22070 0.15 -0.8 5,425,496 + CA265_RS22070 0.16 -1.5 5,425,505 + CA265_RS22070 0.20 -2.8 5,425,546 + CA265_RS22070 0.41 -2.1 5,425,549 - CA265_RS22070 0.42 +2.7 5,425,560 + CA265_RS22070 0.48 -3.6 5,425,631 + CA265_RS22070 0.84 +0.0 5,425,631 + CA265_RS22070 0.84 -1.5 5,425,633 + CA265_RS22070 0.85 -2.6 5,425,633 + CA265_RS22070 0.85 +0.3 5,425,661 - +0.9 5,425,666 + -1.9 5,425,666 + -0.8 5,425,691 - -0.2 5,425,699 + +0.8 5,425,764 + +2.2 5,425,785 - -2.8 5,425,785 - +1.2 5,425,805 - -1.0 5,425,891 + -3.3 5,425,892 - -1.5 5,425,997 + +1.1 5,425,998 - +1.4 5,426,012 + -1.0 5,426,026 + +0.6 5,426,032 - -2.1 5,426,079 + -1.0 5,426,080 - +0.8 5,426,108 + +1.3 5,426,126 - -2.9 5,426,126 - +0.8 5,426,126 - +1.1 5,426,128 - -1.5 5,426,162 + +5.0 5,426,193 - -0.8 5,426,193 - -2.3 5,426,268 + CA265_RS22075 0.20 +0.7 5,426,280 + CA265_RS22075 0.23 -1.5 5,426,281 - CA265_RS22075 0.24 +0.7 5,426,325 + CA265_RS22075 0.34 -1.9 5,426,326 - CA265_RS22075 0.34 +2.0 5,426,326 - CA265_RS22075 0.34 +0.1 5,426,326 - CA265_RS22075 0.34 -2.2 5,426,326 - CA265_RS22075 0.34 +1.6 5,426,327 + CA265_RS22075 0.35 -0.9 5,426,416 - CA265_RS22075 0.56 -0.6 5,426,424 + CA265_RS22075 0.58 -1.0 5,426,460 + CA265_RS22075 0.66 -1.3 5,426,460 + CA265_RS22075 0.66 -2.2 5,426,461 - CA265_RS22075 0.66 -0.7 5,426,540 + +3.5 5,426,608 + +1.5 5,426,672 + -1.7 5,426,672 + +0.5 5,426,677 - -0.5 5,426,749 - +4.5 5,426,751 + -2.3 5,426,751 + +2.5 5,426,762 - -1.1 5,426,818 + CA265_RS22080 0.21 +1.0 5,426,854 - CA265_RS22080 0.30 -0.8 5,426,896 - CA265_RS22080 0.40 +0.7 5,426,955 + CA265_RS22080 0.54 -1.2 5,427,042 + CA265_RS22080 0.75 -2.5 5,427,150 + +1.0 5,427,258 + -0.2 5,427,383 + +0.0 5,427,395 - +0.7 5,427,422 - +0.8 5,427,464 + +0.3 5,427,486 - -0.1 5,427,486 - -1.0
Or see this region's nucleotide sequence