Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22060 and CA265_RS22065 are separated by 493 nucleotides CA265_RS22065 and CA265_RS22070 are separated by 375 nucleotides
CA265_RS22060: CA265_RS22060 - hypothetical protein, at 5,423,334 to 5,424,179
_RS22060
CA265_RS22065: CA265_RS22065 - hypothetical protein, at 5,424,673 to 5,425,089
_RS22065
CA265_RS22070: CA265_RS22070 - hypothetical protein, at 5,425,465 to 5,425,662
_RS22070
Position (kb)
5424
5425
5426 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5424.257 kb on + strand at 5424.258 kb on - strand at 5424.259 kb on + strand at 5424.313 kb on + strand at 5424.356 kb on + strand at 5424.356 kb on + strand at 5424.357 kb on - strand at 5424.380 kb on - strand at 5424.380 kb on - strand at 5424.393 kb on - strand at 5424.457 kb on - strand at 5424.499 kb on - strand at 5424.614 kb on + strand at 5424.614 kb on + strand at 5424.643 kb on + strand at 5424.644 kb on - strand at 5424.657 kb on + strand at 5424.666 kb on - strand at 5424.672 kb on + strand at 5424.673 kb on - strand at 5424.724 kb on - strand, within CA265_RS22065 at 5424.767 kb on + strand, within CA265_RS22065 at 5424.872 kb on + strand, within CA265_RS22065 at 5424.873 kb on - strand, within CA265_RS22065 at 5424.882 kb on - strand, within CA265_RS22065 at 5424.895 kb on - strand, within CA265_RS22065 at 5424.969 kb on - strand, within CA265_RS22065 at 5424.983 kb on - strand, within CA265_RS22065 at 5424.983 kb on - strand, within CA265_RS22065 at 5424.983 kb on - strand, within CA265_RS22065 at 5424.987 kb on - strand, within CA265_RS22065 at 5424.997 kb on - strand, within CA265_RS22065 at 5425.044 kb on - strand, within CA265_RS22065 at 5425.050 kb on - strand at 5425.050 kb on - strand at 5425.234 kb on + strand at 5425.235 kb on - strand at 5425.249 kb on - strand at 5425.292 kb on + strand at 5425.299 kb on - strand at 5425.331 kb on + strand at 5425.332 kb on - strand at 5425.494 kb on + strand, within CA265_RS22070 at 5425.496 kb on + strand, within CA265_RS22070 at 5425.505 kb on + strand, within CA265_RS22070 at 5425.546 kb on + strand, within CA265_RS22070 at 5425.549 kb on - strand, within CA265_RS22070 at 5425.560 kb on + strand, within CA265_RS22070 at 5425.631 kb on + strand, within CA265_RS22070 at 5425.631 kb on + strand, within CA265_RS22070 at 5425.633 kb on + strand, within CA265_RS22070 at 5425.633 kb on + strand, within CA265_RS22070 at 5425.661 kb on - strand at 5425.666 kb on + strand at 5425.666 kb on + strand at 5425.691 kb on - strand at 5425.699 kb on + strand at 5425.764 kb on + strand at 5425.785 kb on - strand at 5425.785 kb on - strand at 5425.805 kb on - strand at 5425.891 kb on + strand at 5425.892 kb on - strand at 5425.997 kb on + strand at 5425.998 kb on - strand at 5426.012 kb on + strand at 5426.026 kb on + strand at 5426.032 kb on - strand at 5426.079 kb on + strand at 5426.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,424,257 + +2.5 5,424,258 - -0.3 5,424,259 + -2.0 5,424,313 + -3.0 5,424,356 + -1.7 5,424,356 + -0.6 5,424,357 - +0.9 5,424,380 - -1.3 5,424,380 - -2.2 5,424,393 - +2.5 5,424,457 - -1.8 5,424,499 - -1.2 5,424,614 + +1.0 5,424,614 + -2.7 5,424,643 + +1.5 5,424,644 - -1.8 5,424,657 + +0.7 5,424,666 - -1.8 5,424,672 + -0.7 5,424,673 - +0.8 5,424,724 - CA265_RS22065 0.12 +0.6 5,424,767 + CA265_RS22065 0.23 -1.8 5,424,872 + CA265_RS22065 0.48 -1.2 5,424,873 - CA265_RS22065 0.48 -0.0 5,424,882 - CA265_RS22065 0.50 +0.9 5,424,895 - CA265_RS22065 0.53 -1.5 5,424,969 - CA265_RS22065 0.71 -0.9 5,424,983 - CA265_RS22065 0.74 +0.5 5,424,983 - CA265_RS22065 0.74 -2.6 5,424,983 - CA265_RS22065 0.74 -0.6 5,424,987 - CA265_RS22065 0.75 -2.0 5,424,997 - CA265_RS22065 0.78 +0.8 5,425,044 - CA265_RS22065 0.89 -1.2 5,425,050 - -0.8 5,425,050 - -1.6 5,425,234 + -1.5 5,425,235 - -1.0 5,425,249 - -1.0 5,425,292 + +0.7 5,425,299 - -1.5 5,425,331 + +0.5 5,425,332 - +0.2 5,425,494 + CA265_RS22070 0.15 -0.8 5,425,496 + CA265_RS22070 0.16 -1.5 5,425,505 + CA265_RS22070 0.20 -2.8 5,425,546 + CA265_RS22070 0.41 -2.1 5,425,549 - CA265_RS22070 0.42 +2.7 5,425,560 + CA265_RS22070 0.48 -3.6 5,425,631 + CA265_RS22070 0.84 +0.0 5,425,631 + CA265_RS22070 0.84 -1.5 5,425,633 + CA265_RS22070 0.85 -2.6 5,425,633 + CA265_RS22070 0.85 +0.3 5,425,661 - +0.9 5,425,666 + -1.9 5,425,666 + -0.8 5,425,691 - -0.2 5,425,699 + +0.8 5,425,764 + +2.2 5,425,785 - -2.8 5,425,785 - +1.2 5,425,805 - -1.0 5,425,891 + -3.3 5,425,892 - -1.5 5,425,997 + +1.1 5,425,998 - +1.4 5,426,012 + -1.0 5,426,026 + +0.6 5,426,032 - -2.1 5,426,079 + -1.0 5,426,080 - +0.8
Or see this region's nucleotide sequence