Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21355 and CA265_RS21360 are separated by 149 nucleotides CA265_RS21360 and CA265_RS21365 overlap by 1 nucleotides CA265_RS21365 and CA265_RS21370 are separated by 14 nucleotides
CA265_RS21355: CA265_RS21355 - hypothetical protein, at 5,269,351 to 5,269,551
_RS21355
CA265_RS21360: CA265_RS21360 - hypothetical protein, at 5,269,701 to 5,269,949
_RS21360
CA265_RS21365: CA265_RS21365 - thiol reductase thioredoxin, at 5,269,949 to 5,270,257
_RS21365
CA265_RS21370: CA265_RS21370 - peroxiredoxin, at 5,270,272 to 5,270,904
_RS21370
Position (kb) 5269
5270
5271 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5269.011 kb on + strand at 5269.030 kb on + strand at 5269.077 kb on - strand at 5269.077 kb on - strand at 5269.084 kb on + strand at 5269.092 kb on + strand at 5269.093 kb on - strand at 5269.093 kb on - strand at 5269.093 kb on - strand at 5269.116 kb on + strand at 5269.120 kb on - strand at 5269.127 kb on + strand at 5269.177 kb on - strand at 5269.209 kb on + strand at 5269.246 kb on - strand at 5269.284 kb on + strand at 5269.315 kb on + strand at 5269.315 kb on + strand at 5269.360 kb on + strand at 5269.434 kb on + strand, within CA265_RS21355 at 5269.442 kb on - strand, within CA265_RS21355 at 5269.453 kb on - strand, within CA265_RS21355 at 5269.508 kb on + strand, within CA265_RS21355 at 5269.513 kb on - strand, within CA265_RS21355 at 5269.516 kb on + strand, within CA265_RS21355 at 5269.557 kb on - strand at 5269.665 kb on + strand at 5269.666 kb on - strand at 5269.743 kb on + strand, within CA265_RS21360 at 5269.785 kb on + strand, within CA265_RS21360 at 5269.785 kb on + strand, within CA265_RS21360 at 5269.977 kb on - strand at 5270.009 kb on + strand, within CA265_RS21365 at 5270.046 kb on - strand, within CA265_RS21365 at 5270.050 kb on + strand, within CA265_RS21365 at 5270.188 kb on - strand, within CA265_RS21365 at 5270.244 kb on - strand at 5270.270 kb on - strand at 5270.402 kb on + strand, within CA265_RS21370 at 5270.572 kb on + strand, within CA265_RS21370 at 5270.587 kb on - strand, within CA265_RS21370 at 5270.604 kb on - strand, within CA265_RS21370 at 5270.607 kb on + strand, within CA265_RS21370 at 5270.607 kb on + strand, within CA265_RS21370 at 5270.640 kb on + strand, within CA265_RS21370 at 5270.641 kb on - strand, within CA265_RS21370 at 5270.645 kb on - strand, within CA265_RS21370 at 5270.671 kb on - strand, within CA265_RS21370 at 5270.671 kb on - strand, within CA265_RS21370 at 5270.697 kb on + strand, within CA265_RS21370 at 5270.789 kb on - strand, within CA265_RS21370 at 5270.825 kb on - strand, within CA265_RS21370 at 5270.938 kb on - strand at 5270.943 kb on + strand at 5270.986 kb on + strand at 5271.005 kb on - strand at 5271.082 kb on + strand at 5271.083 kb on - strand at 5271.146 kb on - strand at 5271.177 kb on + strand at 5271.181 kb on - strand at 5271.181 kb on - strand at 5271.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,269,011 + -0.9 5,269,030 + -2.6 5,269,077 - +1.1 5,269,077 - -0.9 5,269,084 + -1.9 5,269,092 + +0.7 5,269,093 - -0.1 5,269,093 - -2.0 5,269,093 - +1.4 5,269,116 + +0.2 5,269,120 - -1.5 5,269,127 + +0.8 5,269,177 - -1.4 5,269,209 + +3.1 5,269,246 - -2.8 5,269,284 + -0.5 5,269,315 + +0.7 5,269,315 + +1.9 5,269,360 + -0.6 5,269,434 + CA265_RS21355 0.41 +0.4 5,269,442 - CA265_RS21355 0.45 -1.6 5,269,453 - CA265_RS21355 0.51 -0.8 5,269,508 + CA265_RS21355 0.78 +0.5 5,269,513 - CA265_RS21355 0.81 -0.1 5,269,516 + CA265_RS21355 0.82 +0.4 5,269,557 - -0.5 5,269,665 + -0.4 5,269,666 - -1.7 5,269,743 + CA265_RS21360 0.17 -1.1 5,269,785 + CA265_RS21360 0.34 +0.1 5,269,785 + CA265_RS21360 0.34 +0.2 5,269,977 - -2.3 5,270,009 + CA265_RS21365 0.19 +1.2 5,270,046 - CA265_RS21365 0.31 +2.7 5,270,050 + CA265_RS21365 0.33 -0.3 5,270,188 - CA265_RS21365 0.77 -0.5 5,270,244 - +0.5 5,270,270 - -1.1 5,270,402 + CA265_RS21370 0.21 -0.4 5,270,572 + CA265_RS21370 0.47 +0.4 5,270,587 - CA265_RS21370 0.50 -0.3 5,270,604 - CA265_RS21370 0.52 +0.3 5,270,607 + CA265_RS21370 0.53 -3.0 5,270,607 + CA265_RS21370 0.53 -0.5 5,270,640 + CA265_RS21370 0.58 +0.6 5,270,641 - CA265_RS21370 0.58 -1.4 5,270,645 - CA265_RS21370 0.59 +0.9 5,270,671 - CA265_RS21370 0.63 +0.8 5,270,671 - CA265_RS21370 0.63 +0.5 5,270,697 + CA265_RS21370 0.67 +0.9 5,270,789 - CA265_RS21370 0.82 +0.4 5,270,825 - CA265_RS21370 0.87 -0.9 5,270,938 - -2.2 5,270,943 + -1.4 5,270,986 + -1.0 5,271,005 - -0.0 5,271,082 + +0.7 5,271,083 - +0.1 5,271,146 - +1.6 5,271,177 + -1.5 5,271,181 - +0.0 5,271,181 - -0.2 5,271,182 + +0.2
Or see this region's nucleotide sequence