Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20990 and CA265_RS20995 are separated by 7 nucleotides CA265_RS20995 and CA265_RS21000 are separated by 44 nucleotides CA265_RS21000 and CA265_RS21005 are separated by 27 nucleotides
CA265_RS20990: CA265_RS20990 - sugar transporter, at 5,172,013 to 5,172,462
_RS20990
CA265_RS20995: CA265_RS20995 - biotin--[acetyl-CoA-carboxylase] ligase, at 5,172,470 to 5,173,246
_RS20995
CA265_RS21000: CA265_RS21000 - ribosome silencing factor, at 5,173,291 to 5,173,665
_RS21000
CA265_RS21005: CA265_RS21005 - peptidase M41, at 5,173,693 to 5,175,708
_RS21005
Position (kb) 5173
5174 Strain fitness (log2 ratio) -1
0
1
2 at 5172.320 kb on - strand, within CA265_RS20990 at 5172.322 kb on - strand, within CA265_RS20990 at 5172.472 kb on - strand at 5173.220 kb on + strand at 5173.262 kb on + strand at 5173.307 kb on + strand at 5173.320 kb on + strand at 5173.352 kb on + strand, within CA265_RS21000 at 5173.374 kb on + strand, within CA265_RS21000 at 5173.374 kb on + strand, within CA265_RS21000 at 5173.425 kb on + strand, within CA265_RS21000 at 5173.478 kb on + strand, within CA265_RS21000 at 5173.479 kb on - strand, within CA265_RS21000 at 5173.490 kb on + strand, within CA265_RS21000 at 5173.493 kb on - strand at 5173.494 kb on + strand, within CA265_RS21000 at 5173.552 kb on - strand, within CA265_RS21000 at 5173.616 kb on + strand, within CA265_RS21000 at 5173.668 kb on + strand at 5173.670 kb on + strand at 5173.670 kb on + strand at 5174.224 kb on - strand, within CA265_RS21005 at 5174.562 kb on - strand, within CA265_RS21005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 5,172,320 - CA265_RS20990 0.68 -0.9 5,172,322 - CA265_RS20990 0.69 +0.5 5,172,472 - +0.8 5,173,220 + +1.1 5,173,262 + +0.2 5,173,307 + +1.3 5,173,320 + +1.9 5,173,352 + CA265_RS21000 0.16 +1.3 5,173,374 + CA265_RS21000 0.22 +1.6 5,173,374 + CA265_RS21000 0.22 +1.0 5,173,425 + CA265_RS21000 0.36 +2.0 5,173,478 + CA265_RS21000 0.50 +1.0 5,173,479 - CA265_RS21000 0.50 -0.9 5,173,490 + CA265_RS21000 0.53 +1.0 5,173,493 - +0.5 5,173,494 + CA265_RS21000 0.54 +2.0 5,173,552 - CA265_RS21000 0.70 +0.9 5,173,616 + CA265_RS21000 0.87 +2.5 5,173,668 + +1.9 5,173,670 + +1.3 5,173,670 + +1.7 5,174,224 - CA265_RS21005 0.26 -0.1 5,174,562 - CA265_RS21005 0.43 +2.5
Or see this region's nucleotide sequence