Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15615 and CA265_RS15620 are separated by 257 nucleotides CA265_RS15620 and CA265_RS15625 are separated by 6 nucleotides
CA265_RS15615: CA265_RS15615 - malate dehydrogenase, at 3,930,210 to 3,931,295
_RS15615
CA265_RS15620: CA265_RS15620 - beta-1,3-glucosyltransferase, at 3,931,553 to 3,934,225
_RS15620
CA265_RS15625: CA265_RS15625 - alpha-L-fucosidase, at 3,934,232 to 3,935,656
_RS15625
Position (kb)
3931
3932
3933
3934
3935 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3930.592 kb on + strand, within CA265_RS15615 at 3930.624 kb on - strand, within CA265_RS15615 at 3930.660 kb on + strand, within CA265_RS15615 at 3930.731 kb on - strand, within CA265_RS15615 at 3930.736 kb on + strand, within CA265_RS15615 at 3930.915 kb on - strand, within CA265_RS15615 at 3930.944 kb on + strand, within CA265_RS15615 at 3930.945 kb on - strand, within CA265_RS15615 at 3930.975 kb on + strand, within CA265_RS15615 at 3930.976 kb on - strand, within CA265_RS15615 at 3931.131 kb on - strand, within CA265_RS15615 at 3931.131 kb on - strand, within CA265_RS15615 at 3931.165 kb on + strand, within CA265_RS15615 at 3931.293 kb on + strand at 3931.314 kb on + strand at 3931.315 kb on - strand at 3931.334 kb on - strand at 3931.334 kb on - strand at 3931.334 kb on - strand at 3931.347 kb on + strand at 3931.561 kb on + strand at 3931.580 kb on + strand at 3931.584 kb on + strand at 3931.585 kb on - strand at 3931.654 kb on + strand at 3931.670 kb on + strand at 3931.719 kb on - strand at 3931.849 kb on + strand, within CA265_RS15620 at 3931.915 kb on - strand, within CA265_RS15620 at 3931.915 kb on - strand, within CA265_RS15620 at 3931.927 kb on - strand, within CA265_RS15620 at 3931.963 kb on + strand, within CA265_RS15620 at 3931.965 kb on + strand, within CA265_RS15620 at 3932.072 kb on + strand, within CA265_RS15620 at 3932.076 kb on - strand, within CA265_RS15620 at 3932.159 kb on - strand, within CA265_RS15620 at 3932.189 kb on + strand, within CA265_RS15620 at 3932.226 kb on - strand, within CA265_RS15620 at 3932.252 kb on + strand, within CA265_RS15620 at 3932.285 kb on + strand, within CA265_RS15620 at 3932.306 kb on + strand, within CA265_RS15620 at 3932.306 kb on + strand, within CA265_RS15620 at 3932.306 kb on + strand, within CA265_RS15620 at 3932.331 kb on - strand, within CA265_RS15620 at 3932.362 kb on + strand, within CA265_RS15620 at 3932.367 kb on + strand, within CA265_RS15620 at 3932.368 kb on - strand, within CA265_RS15620 at 3932.470 kb on + strand, within CA265_RS15620 at 3932.471 kb on - strand, within CA265_RS15620 at 3932.494 kb on + strand, within CA265_RS15620 at 3932.577 kb on - strand, within CA265_RS15620 at 3932.607 kb on + strand, within CA265_RS15620 at 3932.608 kb on - strand, within CA265_RS15620 at 3932.608 kb on - strand, within CA265_RS15620 at 3932.643 kb on - strand, within CA265_RS15620 at 3932.718 kb on + strand, within CA265_RS15620 at 3932.769 kb on - strand, within CA265_RS15620 at 3932.769 kb on - strand, within CA265_RS15620 at 3932.808 kb on - strand, within CA265_RS15620 at 3932.812 kb on - strand, within CA265_RS15620 at 3932.816 kb on - strand, within CA265_RS15620 at 3932.869 kb on + strand, within CA265_RS15620 at 3932.930 kb on + strand, within CA265_RS15620 at 3932.931 kb on - strand, within CA265_RS15620 at 3932.931 kb on - strand, within CA265_RS15620 at 3933.025 kb on + strand, within CA265_RS15620 at 3933.025 kb on + strand, within CA265_RS15620 at 3933.039 kb on - strand, within CA265_RS15620 at 3933.056 kb on - strand, within CA265_RS15620 at 3933.065 kb on + strand, within CA265_RS15620 at 3933.065 kb on + strand, within CA265_RS15620 at 3933.066 kb on - strand, within CA265_RS15620 at 3933.069 kb on + strand, within CA265_RS15620 at 3933.070 kb on - strand, within CA265_RS15620 at 3933.131 kb on + strand, within CA265_RS15620 at 3933.132 kb on - strand, within CA265_RS15620 at 3933.192 kb on + strand, within CA265_RS15620 at 3933.197 kb on + strand, within CA265_RS15620 at 3933.250 kb on + strand, within CA265_RS15620 at 3933.251 kb on - strand, within CA265_RS15620 at 3933.252 kb on + strand, within CA265_RS15620 at 3933.276 kb on - strand, within CA265_RS15620 at 3933.322 kb on + strand, within CA265_RS15620 at 3933.365 kb on - strand, within CA265_RS15620 at 3933.397 kb on + strand, within CA265_RS15620 at 3933.399 kb on + strand, within CA265_RS15620 at 3933.399 kb on + strand, within CA265_RS15620 at 3933.400 kb on - strand, within CA265_RS15620 at 3933.481 kb on + strand, within CA265_RS15620 at 3933.553 kb on + strand, within CA265_RS15620 at 3933.580 kb on + strand, within CA265_RS15620 at 3933.625 kb on - strand, within CA265_RS15620 at 3933.650 kb on + strand, within CA265_RS15620 at 3933.650 kb on + strand, within CA265_RS15620 at 3933.651 kb on - strand, within CA265_RS15620 at 3933.743 kb on + strand, within CA265_RS15620 at 3933.930 kb on + strand, within CA265_RS15620 at 3934.003 kb on + strand at 3934.029 kb on - strand at 3934.049 kb on + strand at 3934.049 kb on + strand at 3934.050 kb on - strand at 3934.050 kb on - strand at 3934.097 kb on + strand at 3934.200 kb on + strand at 3934.202 kb on + strand at 3934.203 kb on - strand at 3934.226 kb on + strand at 3934.229 kb on + strand at 3934.301 kb on + strand at 3934.301 kb on + strand at 3934.301 kb on + strand at 3934.346 kb on + strand at 3934.378 kb on + strand, within CA265_RS15625 at 3934.379 kb on - strand, within CA265_RS15625 at 3934.426 kb on + strand, within CA265_RS15625 at 3934.540 kb on + strand, within CA265_RS15625 at 3934.544 kb on + strand, within CA265_RS15625 at 3934.578 kb on - strand, within CA265_RS15625 at 3934.634 kb on + strand, within CA265_RS15625 at 3934.635 kb on - strand, within CA265_RS15625 at 3934.829 kb on + strand, within CA265_RS15625 at 3934.862 kb on - strand, within CA265_RS15625 at 3934.862 kb on - strand, within CA265_RS15625 at 3934.862 kb on - strand, within CA265_RS15625 at 3934.908 kb on - strand, within CA265_RS15625 at 3934.908 kb on - strand, within CA265_RS15625 at 3934.919 kb on + strand, within CA265_RS15625 at 3934.937 kb on + strand, within CA265_RS15625 at 3934.938 kb on - strand, within CA265_RS15625 at 3934.977 kb on + strand, within CA265_RS15625 at 3934.989 kb on - strand, within CA265_RS15625 at 3935.013 kb on + strand at 3935.020 kb on + strand, within CA265_RS15625 at 3935.058 kb on + strand, within CA265_RS15625 at 3935.059 kb on - strand, within CA265_RS15625 at 3935.085 kb on + strand, within CA265_RS15625 at 3935.085 kb on + strand, within CA265_RS15625 at 3935.103 kb on + strand, within CA265_RS15625 at 3935.104 kb on - strand, within CA265_RS15625 at 3935.178 kb on + strand, within CA265_RS15625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 3,930,592 + CA265_RS15615 0.35 +1.9 3,930,624 - CA265_RS15615 0.38 +1.4 3,930,660 + CA265_RS15615 0.41 -0.2 3,930,731 - CA265_RS15615 0.48 -0.9 3,930,736 + CA265_RS15615 0.48 -1.0 3,930,915 - CA265_RS15615 0.65 -1.6 3,930,944 + CA265_RS15615 0.68 -1.1 3,930,945 - CA265_RS15615 0.68 -1.2 3,930,975 + CA265_RS15615 0.70 -1.8 3,930,976 - CA265_RS15615 0.71 -0.7 3,931,131 - CA265_RS15615 0.85 +0.7 3,931,131 - CA265_RS15615 0.85 +0.5 3,931,165 + CA265_RS15615 0.88 -0.7 3,931,293 + +0.8 3,931,314 + +0.5 3,931,315 - +1.4 3,931,334 - -1.2 3,931,334 - +0.5 3,931,334 - +0.3 3,931,347 + +0.8 3,931,561 + +0.9 3,931,580 + +0.3 3,931,584 + -2.0 3,931,585 - -2.3 3,931,654 + -0.0 3,931,670 + +1.4 3,931,719 - +0.2 3,931,849 + CA265_RS15620 0.11 +0.7 3,931,915 - CA265_RS15620 0.14 +0.0 3,931,915 - CA265_RS15620 0.14 +0.2 3,931,927 - CA265_RS15620 0.14 -2.6 3,931,963 + CA265_RS15620 0.15 -1.3 3,931,965 + CA265_RS15620 0.15 -1.3 3,932,072 + CA265_RS15620 0.19 -1.5 3,932,076 - CA265_RS15620 0.20 +1.3 3,932,159 - CA265_RS15620 0.23 +0.1 3,932,189 + CA265_RS15620 0.24 +0.5 3,932,226 - CA265_RS15620 0.25 -1.2 3,932,252 + CA265_RS15620 0.26 +2.1 3,932,285 + CA265_RS15620 0.27 -0.2 3,932,306 + CA265_RS15620 0.28 +0.2 3,932,306 + CA265_RS15620 0.28 -0.1 3,932,306 + CA265_RS15620 0.28 -0.3 3,932,331 - CA265_RS15620 0.29 +0.8 3,932,362 + CA265_RS15620 0.30 +1.3 3,932,367 + CA265_RS15620 0.30 +0.4 3,932,368 - CA265_RS15620 0.30 -0.2 3,932,470 + CA265_RS15620 0.34 +1.5 3,932,471 - CA265_RS15620 0.34 -0.9 3,932,494 + CA265_RS15620 0.35 +1.7 3,932,577 - CA265_RS15620 0.38 +0.7 3,932,607 + CA265_RS15620 0.39 -1.8 3,932,608 - CA265_RS15620 0.39 -1.6 3,932,608 - CA265_RS15620 0.39 -0.2 3,932,643 - CA265_RS15620 0.41 -0.1 3,932,718 + CA265_RS15620 0.44 -1.3 3,932,769 - CA265_RS15620 0.45 -2.9 3,932,769 - CA265_RS15620 0.45 +0.4 3,932,808 - CA265_RS15620 0.47 +0.7 3,932,812 - CA265_RS15620 0.47 +1.5 3,932,816 - CA265_RS15620 0.47 -1.9 3,932,869 + CA265_RS15620 0.49 -1.3 3,932,930 + CA265_RS15620 0.52 +1.1 3,932,931 - CA265_RS15620 0.52 +0.1 3,932,931 - CA265_RS15620 0.52 +0.4 3,933,025 + CA265_RS15620 0.55 +0.5 3,933,025 + CA265_RS15620 0.55 -0.7 3,933,039 - CA265_RS15620 0.56 -0.5 3,933,056 - CA265_RS15620 0.56 -2.6 3,933,065 + CA265_RS15620 0.57 +0.6 3,933,065 + CA265_RS15620 0.57 -1.8 3,933,066 - CA265_RS15620 0.57 +2.9 3,933,069 + CA265_RS15620 0.57 +3.1 3,933,070 - CA265_RS15620 0.57 -3.6 3,933,131 + CA265_RS15620 0.59 +0.9 3,933,132 - CA265_RS15620 0.59 -0.0 3,933,192 + CA265_RS15620 0.61 -0.1 3,933,197 + CA265_RS15620 0.62 -0.1 3,933,250 + CA265_RS15620 0.63 +1.2 3,933,251 - CA265_RS15620 0.64 +1.0 3,933,252 + CA265_RS15620 0.64 +0.4 3,933,276 - CA265_RS15620 0.64 -0.6 3,933,322 + CA265_RS15620 0.66 -0.3 3,933,365 - CA265_RS15620 0.68 +0.3 3,933,397 + CA265_RS15620 0.69 -2.6 3,933,399 + CA265_RS15620 0.69 +1.0 3,933,399 + CA265_RS15620 0.69 -0.0 3,933,400 - CA265_RS15620 0.69 +2.3 3,933,481 + CA265_RS15620 0.72 -3.5 3,933,553 + CA265_RS15620 0.75 +1.2 3,933,580 + CA265_RS15620 0.76 +0.4 3,933,625 - CA265_RS15620 0.78 +0.2 3,933,650 + CA265_RS15620 0.78 -0.2 3,933,650 + CA265_RS15620 0.78 +1.4 3,933,651 - CA265_RS15620 0.78 -0.5 3,933,743 + CA265_RS15620 0.82 +1.2 3,933,930 + CA265_RS15620 0.89 -0.2 3,934,003 + -0.5 3,934,029 - +2.4 3,934,049 + -0.1 3,934,049 + +1.1 3,934,050 - -1.7 3,934,050 - -2.6 3,934,097 + +0.4 3,934,200 + -0.2 3,934,202 + -0.7 3,934,203 - +0.1 3,934,226 + -0.2 3,934,229 + +0.9 3,934,301 + -1.3 3,934,301 + +0.4 3,934,301 + -0.5 3,934,346 + +0.5 3,934,378 + CA265_RS15625 0.10 -1.1 3,934,379 - CA265_RS15625 0.10 -1.0 3,934,426 + CA265_RS15625 0.14 +0.3 3,934,540 + CA265_RS15625 0.22 +1.1 3,934,544 + CA265_RS15625 0.22 -0.5 3,934,578 - CA265_RS15625 0.24 -1.5 3,934,634 + CA265_RS15625 0.28 +0.4 3,934,635 - CA265_RS15625 0.28 -1.0 3,934,829 + CA265_RS15625 0.42 -1.4 3,934,862 - CA265_RS15625 0.44 -1.4 3,934,862 - CA265_RS15625 0.44 -3.2 3,934,862 - CA265_RS15625 0.44 -0.3 3,934,908 - CA265_RS15625 0.47 -2.5 3,934,908 - CA265_RS15625 0.47 -0.7 3,934,919 + CA265_RS15625 0.48 -0.3 3,934,937 + CA265_RS15625 0.49 -0.6 3,934,938 - CA265_RS15625 0.50 -1.2 3,934,977 + CA265_RS15625 0.52 +0.4 3,934,989 - CA265_RS15625 0.53 +0.6 3,935,013 + -0.5 3,935,020 + CA265_RS15625 0.55 -0.9 3,935,058 + CA265_RS15625 0.58 +0.1 3,935,059 - CA265_RS15625 0.58 -0.6 3,935,085 + CA265_RS15625 0.60 -1.1 3,935,085 + CA265_RS15625 0.60 -2.2 3,935,103 + CA265_RS15625 0.61 +0.8 3,935,104 - CA265_RS15625 0.61 -0.3 3,935,178 + CA265_RS15625 0.66 +0.6
Or see this region's nucleotide sequence