Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14215 and CA265_RS14220 are separated by 208 nucleotides CA265_RS14220 and CA265_RS14225 overlap by 8 nucleotides
CA265_RS14215: CA265_RS14215 - hypothetical protein, at 3,583,135 to 3,587,175
_RS14215
CA265_RS14220: CA265_RS14220 - hypothetical protein, at 3,587,384 to 3,589,081
_RS14220
CA265_RS14225: CA265_RS14225 - hypothetical protein, at 3,589,074 to 3,590,210
_RS14225
Position (kb)
3587
3588
3589
3590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3586.423 kb on + strand, within CA265_RS14215 at 3586.458 kb on - strand, within CA265_RS14215 at 3586.470 kb on + strand, within CA265_RS14215 at 3586.497 kb on + strand, within CA265_RS14215 at 3586.499 kb on + strand, within CA265_RS14215 at 3586.499 kb on + strand, within CA265_RS14215 at 3586.499 kb on + strand, within CA265_RS14215 at 3586.520 kb on + strand, within CA265_RS14215 at 3586.521 kb on - strand, within CA265_RS14215 at 3586.561 kb on - strand, within CA265_RS14215 at 3586.564 kb on - strand, within CA265_RS14215 at 3586.574 kb on + strand, within CA265_RS14215 at 3586.575 kb on - strand, within CA265_RS14215 at 3586.575 kb on - strand, within CA265_RS14215 at 3586.676 kb on - strand, within CA265_RS14215 at 3586.717 kb on + strand, within CA265_RS14215 at 3586.717 kb on + strand, within CA265_RS14215 at 3586.717 kb on + strand, within CA265_RS14215 at 3586.720 kb on - strand, within CA265_RS14215 at 3586.720 kb on - strand, within CA265_RS14215 at 3586.842 kb on + strand at 3586.876 kb on - strand at 3586.963 kb on + strand at 3586.997 kb on + strand at 3587.066 kb on + strand at 3587.067 kb on - strand at 3587.081 kb on - strand at 3587.188 kb on - strand at 3587.188 kb on - strand at 3587.238 kb on + strand at 3587.276 kb on + strand at 3587.277 kb on - strand at 3587.293 kb on + strand at 3587.294 kb on - strand at 3587.297 kb on + strand at 3587.328 kb on - strand at 3587.395 kb on - strand at 3587.420 kb on + strand at 3587.450 kb on - strand at 3587.455 kb on + strand at 3587.487 kb on + strand at 3587.513 kb on - strand at 3587.592 kb on + strand, within CA265_RS14220 at 3587.679 kb on + strand, within CA265_RS14220 at 3587.721 kb on + strand, within CA265_RS14220 at 3587.725 kb on + strand, within CA265_RS14220 at 3587.799 kb on + strand, within CA265_RS14220 at 3587.799 kb on + strand, within CA265_RS14220 at 3587.799 kb on + strand, within CA265_RS14220 at 3587.800 kb on - strand, within CA265_RS14220 at 3587.803 kb on + strand, within CA265_RS14220 at 3587.803 kb on + strand, within CA265_RS14220 at 3587.806 kb on + strand, within CA265_RS14220 at 3587.860 kb on + strand, within CA265_RS14220 at 3587.863 kb on - strand, within CA265_RS14220 at 3587.894 kb on + strand, within CA265_RS14220 at 3587.894 kb on + strand, within CA265_RS14220 at 3587.895 kb on - strand, within CA265_RS14220 at 3587.896 kb on + strand, within CA265_RS14220 at 3587.909 kb on - strand, within CA265_RS14220 at 3587.955 kb on + strand, within CA265_RS14220 at 3587.959 kb on - strand, within CA265_RS14220 at 3588.036 kb on + strand, within CA265_RS14220 at 3588.086 kb on + strand, within CA265_RS14220 at 3588.228 kb on - strand, within CA265_RS14220 at 3588.229 kb on + strand, within CA265_RS14220 at 3588.230 kb on - strand, within CA265_RS14220 at 3588.230 kb on - strand, within CA265_RS14220 at 3588.241 kb on + strand, within CA265_RS14220 at 3588.245 kb on + strand, within CA265_RS14220 at 3588.258 kb on + strand, within CA265_RS14220 at 3588.274 kb on + strand, within CA265_RS14220 at 3588.275 kb on - strand, within CA265_RS14220 at 3588.295 kb on + strand, within CA265_RS14220 at 3588.295 kb on + strand, within CA265_RS14220 at 3588.296 kb on - strand, within CA265_RS14220 at 3588.296 kb on - strand, within CA265_RS14220 at 3588.298 kb on + strand, within CA265_RS14220 at 3588.298 kb on + strand, within CA265_RS14220 at 3588.299 kb on - strand, within CA265_RS14220 at 3588.326 kb on + strand, within CA265_RS14220 at 3588.327 kb on - strand, within CA265_RS14220 at 3588.361 kb on + strand, within CA265_RS14220 at 3588.362 kb on - strand, within CA265_RS14220 at 3588.386 kb on + strand, within CA265_RS14220 at 3588.386 kb on + strand, within CA265_RS14220 at 3588.386 kb on + strand, within CA265_RS14220 at 3588.388 kb on + strand, within CA265_RS14220 at 3588.461 kb on + strand, within CA265_RS14220 at 3588.461 kb on + strand, within CA265_RS14220 at 3588.484 kb on + strand, within CA265_RS14220 at 3588.530 kb on - strand, within CA265_RS14220 at 3588.530 kb on - strand, within CA265_RS14220 at 3588.560 kb on + strand, within CA265_RS14220 at 3588.561 kb on - strand, within CA265_RS14220 at 3588.561 kb on - strand, within CA265_RS14220 at 3588.573 kb on + strand, within CA265_RS14220 at 3588.631 kb on - strand, within CA265_RS14220 at 3588.773 kb on + strand, within CA265_RS14220 at 3588.774 kb on - strand, within CA265_RS14220 at 3588.777 kb on + strand, within CA265_RS14220 at 3588.788 kb on + strand, within CA265_RS14220 at 3588.789 kb on - strand, within CA265_RS14220 at 3588.791 kb on - strand, within CA265_RS14220 at 3588.864 kb on - strand, within CA265_RS14220 at 3588.951 kb on - strand at 3588.951 kb on - strand at 3589.072 kb on - strand at 3589.115 kb on + strand at 3589.115 kb on + strand at 3589.116 kb on - strand at 3589.140 kb on + strand at 3589.141 kb on - strand at 3589.185 kb on - strand at 3589.191 kb on + strand, within CA265_RS14225 at 3589.191 kb on + strand, within CA265_RS14225 at 3589.192 kb on - strand, within CA265_RS14225 at 3589.202 kb on - strand, within CA265_RS14225 at 3589.239 kb on + strand, within CA265_RS14225 at 3589.362 kb on + strand, within CA265_RS14225 at 3589.362 kb on + strand, within CA265_RS14225 at 3589.363 kb on - strand, within CA265_RS14225 at 3589.370 kb on - strand, within CA265_RS14225 at 3589.420 kb on + strand, within CA265_RS14225 at 3589.428 kb on + strand, within CA265_RS14225 at 3589.474 kb on - strand, within CA265_RS14225 at 3589.560 kb on + strand, within CA265_RS14225 at 3589.561 kb on - strand, within CA265_RS14225 at 3589.561 kb on - strand, within CA265_RS14225 at 3589.566 kb on - strand, within CA265_RS14225 at 3589.585 kb on + strand, within CA265_RS14225 at 3589.615 kb on + strand, within CA265_RS14225 at 3589.656 kb on - strand, within CA265_RS14225 at 3589.665 kb on - strand, within CA265_RS14225 at 3589.711 kb on + strand, within CA265_RS14225 at 3589.839 kb on - strand, within CA265_RS14225 at 3589.861 kb on - strand, within CA265_RS14225 at 3589.862 kb on + strand, within CA265_RS14225 at 3590.001 kb on + strand, within CA265_RS14225 at 3590.002 kb on - strand, within CA265_RS14225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 3,586,423 + CA265_RS14215 0.81 +1.8 3,586,458 - CA265_RS14215 0.82 +0.9 3,586,470 + CA265_RS14215 0.83 +0.4 3,586,497 + CA265_RS14215 0.83 -2.1 3,586,499 + CA265_RS14215 0.83 +0.7 3,586,499 + CA265_RS14215 0.83 +0.1 3,586,499 + CA265_RS14215 0.83 -0.4 3,586,520 + CA265_RS14215 0.84 +1.1 3,586,521 - CA265_RS14215 0.84 +0.8 3,586,561 - CA265_RS14215 0.85 +1.1 3,586,564 - CA265_RS14215 0.85 +0.8 3,586,574 + CA265_RS14215 0.85 -1.2 3,586,575 - CA265_RS14215 0.85 -0.2 3,586,575 - CA265_RS14215 0.85 -0.3 3,586,676 - CA265_RS14215 0.88 -0.6 3,586,717 + CA265_RS14215 0.89 -1.2 3,586,717 + CA265_RS14215 0.89 -0.2 3,586,717 + CA265_RS14215 0.89 +0.1 3,586,720 - CA265_RS14215 0.89 -0.8 3,586,720 - CA265_RS14215 0.89 -0.3 3,586,842 + +0.6 3,586,876 - -0.9 3,586,963 + +0.9 3,586,997 + +3.7 3,587,066 + +0.9 3,587,067 - -0.4 3,587,081 - -0.1 3,587,188 - -0.2 3,587,188 - -1.4 3,587,238 + -0.5 3,587,276 + +1.7 3,587,277 - +0.7 3,587,293 + +0.4 3,587,294 - -0.1 3,587,297 + +0.0 3,587,328 - +0.8 3,587,395 - -0.5 3,587,420 + -1.9 3,587,450 - -0.8 3,587,455 + +0.0 3,587,487 + +0.0 3,587,513 - -0.3 3,587,592 + CA265_RS14220 0.12 +0.5 3,587,679 + CA265_RS14220 0.17 +1.7 3,587,721 + CA265_RS14220 0.20 +1.8 3,587,725 + CA265_RS14220 0.20 -0.6 3,587,799 + CA265_RS14220 0.24 -1.8 3,587,799 + CA265_RS14220 0.24 -0.8 3,587,799 + CA265_RS14220 0.24 -0.2 3,587,800 - CA265_RS14220 0.24 +1.5 3,587,803 + CA265_RS14220 0.25 -0.5 3,587,803 + CA265_RS14220 0.25 -0.1 3,587,806 + CA265_RS14220 0.25 +0.5 3,587,860 + CA265_RS14220 0.28 +0.3 3,587,863 - CA265_RS14220 0.28 -1.4 3,587,894 + CA265_RS14220 0.30 +1.2 3,587,894 + CA265_RS14220 0.30 -2.4 3,587,895 - CA265_RS14220 0.30 +0.8 3,587,896 + CA265_RS14220 0.30 +0.4 3,587,909 - CA265_RS14220 0.31 +0.5 3,587,955 + CA265_RS14220 0.34 +0.0 3,587,959 - CA265_RS14220 0.34 -0.2 3,588,036 + CA265_RS14220 0.38 +1.2 3,588,086 + CA265_RS14220 0.41 -0.4 3,588,228 - CA265_RS14220 0.50 -1.9 3,588,229 + CA265_RS14220 0.50 +0.5 3,588,230 - CA265_RS14220 0.50 -0.4 3,588,230 - CA265_RS14220 0.50 +1.5 3,588,241 + CA265_RS14220 0.50 -0.1 3,588,245 + CA265_RS14220 0.51 -1.9 3,588,258 + CA265_RS14220 0.51 -2.9 3,588,274 + CA265_RS14220 0.52 -2.4 3,588,275 - CA265_RS14220 0.52 +0.0 3,588,295 + CA265_RS14220 0.54 -0.6 3,588,295 + CA265_RS14220 0.54 -1.3 3,588,296 - CA265_RS14220 0.54 -1.8 3,588,296 - CA265_RS14220 0.54 +0.2 3,588,298 + CA265_RS14220 0.54 -1.0 3,588,298 + CA265_RS14220 0.54 -1.5 3,588,299 - CA265_RS14220 0.54 +0.5 3,588,326 + CA265_RS14220 0.55 -0.0 3,588,327 - CA265_RS14220 0.56 -0.1 3,588,361 + CA265_RS14220 0.58 -1.2 3,588,362 - CA265_RS14220 0.58 -0.4 3,588,386 + CA265_RS14220 0.59 -1.8 3,588,386 + CA265_RS14220 0.59 +1.0 3,588,386 + CA265_RS14220 0.59 -1.8 3,588,388 + CA265_RS14220 0.59 -2.4 3,588,461 + CA265_RS14220 0.63 +0.1 3,588,461 + CA265_RS14220 0.63 -2.8 3,588,484 + CA265_RS14220 0.65 +0.8 3,588,530 - CA265_RS14220 0.67 +0.4 3,588,530 - CA265_RS14220 0.67 +0.6 3,588,560 + CA265_RS14220 0.69 +0.2 3,588,561 - CA265_RS14220 0.69 +0.9 3,588,561 - CA265_RS14220 0.69 -0.7 3,588,573 + CA265_RS14220 0.70 -1.0 3,588,631 - CA265_RS14220 0.73 +0.0 3,588,773 + CA265_RS14220 0.82 -0.6 3,588,774 - CA265_RS14220 0.82 +0.4 3,588,777 + CA265_RS14220 0.82 -0.9 3,588,788 + CA265_RS14220 0.83 -0.2 3,588,789 - CA265_RS14220 0.83 +0.4 3,588,791 - CA265_RS14220 0.83 -1.4 3,588,864 - CA265_RS14220 0.87 +0.1 3,588,951 - -1.0 3,588,951 - -0.5 3,589,072 - -0.5 3,589,115 + +0.1 3,589,115 + -1.0 3,589,116 - +0.7 3,589,140 + -1.0 3,589,141 - +0.8 3,589,185 - +1.0 3,589,191 + CA265_RS14225 0.10 -0.2 3,589,191 + CA265_RS14225 0.10 +0.2 3,589,192 - CA265_RS14225 0.10 -3.3 3,589,202 - CA265_RS14225 0.11 +0.3 3,589,239 + CA265_RS14225 0.15 -2.6 3,589,362 + CA265_RS14225 0.25 -2.2 3,589,362 + CA265_RS14225 0.25 +0.3 3,589,363 - CA265_RS14225 0.25 -0.9 3,589,370 - CA265_RS14225 0.26 +0.5 3,589,420 + CA265_RS14225 0.30 -0.2 3,589,428 + CA265_RS14225 0.31 +0.9 3,589,474 - CA265_RS14225 0.35 -0.3 3,589,560 + CA265_RS14225 0.43 +1.0 3,589,561 - CA265_RS14225 0.43 +0.3 3,589,561 - CA265_RS14225 0.43 -3.1 3,589,566 - CA265_RS14225 0.43 -1.3 3,589,585 + CA265_RS14225 0.45 -0.3 3,589,615 + CA265_RS14225 0.48 +0.1 3,589,656 - CA265_RS14225 0.51 -0.1 3,589,665 - CA265_RS14225 0.52 -2.6 3,589,711 + CA265_RS14225 0.56 -1.9 3,589,839 - CA265_RS14225 0.67 -0.5 3,589,861 - CA265_RS14225 0.69 +0.3 3,589,862 + CA265_RS14225 0.69 -0.3 3,590,001 + CA265_RS14225 0.82 -0.3 3,590,002 - CA265_RS14225 0.82 +0.0
Or see this region's nucleotide sequence