Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11410 and CA265_RS11415 are separated by 28 nucleotides CA265_RS11415 and CA265_RS11420 are separated by 41 nucleotides CA265_RS11420 and CA265_RS11425 are separated by 0 nucleotides
CA265_RS11410: CA265_RS11410 - dihydrofolate reductase, at 2,834,973 to 2,835,527
_RS11410
CA265_RS11415: CA265_RS11415 - glyoxalase, at 2,835,556 to 2,835,945
_RS11415
CA265_RS11420: CA265_RS11420 - hypothetical protein, at 2,835,987 to 2,836,583
_RS11420
CA265_RS11425: CA265_RS11425 - hypothetical protein, at 2,836,584 to 2,839,304
_RS11425
Position (kb)
2835
2836
2837 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2835.018 kb on + strand at 2835.018 kb on + strand at 2835.224 kb on + strand, within CA265_RS11410 at 2835.278 kb on + strand, within CA265_RS11410 at 2835.278 kb on + strand, within CA265_RS11410 at 2835.279 kb on - strand, within CA265_RS11410 at 2835.296 kb on - strand, within CA265_RS11410 at 2835.387 kb on + strand, within CA265_RS11410 at 2835.470 kb on - strand, within CA265_RS11410 at 2835.470 kb on - strand, within CA265_RS11410 at 2835.503 kb on - strand at 2835.514 kb on - strand at 2835.538 kb on - strand at 2835.551 kb on + strand at 2835.552 kb on - strand at 2835.555 kb on + strand at 2835.555 kb on + strand at 2835.665 kb on + strand, within CA265_RS11415 at 2835.721 kb on - strand, within CA265_RS11415 at 2835.783 kb on + strand, within CA265_RS11415 at 2835.813 kb on - strand, within CA265_RS11415 at 2835.854 kb on + strand, within CA265_RS11415 at 2835.886 kb on + strand, within CA265_RS11415 at 2835.887 kb on - strand, within CA265_RS11415 at 2835.887 kb on - strand, within CA265_RS11415 at 2835.925 kb on - strand at 2835.927 kb on + strand at 2835.932 kb on + strand at 2835.943 kb on + strand at 2835.948 kb on - strand at 2835.951 kb on + strand at 2835.951 kb on + strand at 2836.001 kb on + strand at 2836.014 kb on - strand at 2836.014 kb on - strand at 2836.077 kb on + strand, within CA265_RS11420 at 2836.077 kb on + strand, within CA265_RS11420 at 2836.101 kb on + strand, within CA265_RS11420 at 2836.129 kb on - strand, within CA265_RS11420 at 2836.343 kb on - strand, within CA265_RS11420 at 2836.346 kb on + strand, within CA265_RS11420 at 2836.347 kb on - strand, within CA265_RS11420 at 2836.347 kb on - strand, within CA265_RS11420 at 2836.390 kb on - strand, within CA265_RS11420 at 2836.422 kb on - strand, within CA265_RS11420 at 2836.436 kb on + strand, within CA265_RS11420 at 2836.436 kb on + strand, within CA265_RS11420 at 2836.458 kb on - strand, within CA265_RS11420 at 2836.458 kb on - strand, within CA265_RS11420 at 2836.471 kb on - strand at 2836.471 kb on - strand, within CA265_RS11420 at 2836.574 kb on + strand at 2836.575 kb on - strand at 2836.575 kb on - strand at 2836.626 kb on + strand at 2836.638 kb on + strand at 2836.769 kb on + strand at 2836.769 kb on + strand at 2836.770 kb on - strand at 2836.775 kb on - strand at 2836.775 kb on - strand at 2836.862 kb on + strand, within CA265_RS11425 at 2836.899 kb on - strand, within CA265_RS11425 at 2836.941 kb on - strand, within CA265_RS11425 at 2836.990 kb on - strand, within CA265_RS11425 at 2837.081 kb on + strand, within CA265_RS11425 at 2837.252 kb on + strand, within CA265_RS11425 at 2837.253 kb on - strand, within CA265_RS11425 at 2837.254 kb on + strand, within CA265_RS11425 at 2837.255 kb on - strand, within CA265_RS11425 at 2837.274 kb on - strand, within CA265_RS11425 at 2837.395 kb on + strand, within CA265_RS11425 at 2837.442 kb on - strand, within CA265_RS11425 at 2837.526 kb on - strand, within CA265_RS11425 at 2837.532 kb on + strand, within CA265_RS11425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 2,835,018 + -2.0 2,835,018 + +1.6 2,835,224 + CA265_RS11410 0.45 +0.1 2,835,278 + CA265_RS11410 0.55 +0.2 2,835,278 + CA265_RS11410 0.55 -2.2 2,835,279 - CA265_RS11410 0.55 -1.6 2,835,296 - CA265_RS11410 0.58 +1.4 2,835,387 + CA265_RS11410 0.75 -2.4 2,835,470 - CA265_RS11410 0.90 -1.8 2,835,470 - CA265_RS11410 0.90 +0.0 2,835,503 - +0.6 2,835,514 - +1.5 2,835,538 - +1.3 2,835,551 + -0.6 2,835,552 - -1.9 2,835,555 + -0.4 2,835,555 + +0.5 2,835,665 + CA265_RS11415 0.28 -1.5 2,835,721 - CA265_RS11415 0.42 -1.2 2,835,783 + CA265_RS11415 0.58 -1.2 2,835,813 - CA265_RS11415 0.66 -3.1 2,835,854 + CA265_RS11415 0.76 -2.2 2,835,886 + CA265_RS11415 0.85 -3.8 2,835,887 - CA265_RS11415 0.85 +0.1 2,835,887 - CA265_RS11415 0.85 -2.5 2,835,925 - -0.8 2,835,927 + +0.6 2,835,932 + -1.9 2,835,943 + -2.3 2,835,948 - +0.9 2,835,951 + -0.3 2,835,951 + -0.6 2,836,001 + -0.1 2,836,014 - -0.8 2,836,014 - -2.0 2,836,077 + CA265_RS11420 0.15 -2.1 2,836,077 + CA265_RS11420 0.15 -0.1 2,836,101 + CA265_RS11420 0.19 -0.2 2,836,129 - CA265_RS11420 0.24 +0.7 2,836,343 - CA265_RS11420 0.60 +0.4 2,836,346 + CA265_RS11420 0.60 -2.0 2,836,347 - CA265_RS11420 0.60 -1.5 2,836,347 - CA265_RS11420 0.60 -0.4 2,836,390 - CA265_RS11420 0.68 -0.9 2,836,422 - CA265_RS11420 0.73 -0.5 2,836,436 + CA265_RS11420 0.75 +0.6 2,836,436 + CA265_RS11420 0.75 -1.0 2,836,458 - CA265_RS11420 0.79 -1.0 2,836,458 - CA265_RS11420 0.79 -0.5 2,836,471 - -1.0 2,836,471 - CA265_RS11420 0.81 -4.1 2,836,574 + +0.2 2,836,575 - -2.3 2,836,575 - -0.2 2,836,626 + +0.6 2,836,638 + +0.1 2,836,769 + -0.0 2,836,769 + -1.2 2,836,770 - -1.2 2,836,775 - +0.3 2,836,775 - +1.0 2,836,862 + CA265_RS11425 0.10 +0.0 2,836,899 - CA265_RS11425 0.12 +1.6 2,836,941 - CA265_RS11425 0.13 -0.9 2,836,990 - CA265_RS11425 0.15 -1.0 2,837,081 + CA265_RS11425 0.18 -1.0 2,837,252 + CA265_RS11425 0.25 +0.2 2,837,253 - CA265_RS11425 0.25 -1.9 2,837,254 + CA265_RS11425 0.25 -1.9 2,837,255 - CA265_RS11425 0.25 +0.3 2,837,274 - CA265_RS11425 0.25 -1.3 2,837,395 + CA265_RS11425 0.30 +1.2 2,837,442 - CA265_RS11425 0.32 +0.5 2,837,526 - CA265_RS11425 0.35 -2.5 2,837,532 + CA265_RS11425 0.35 -0.7
Or see this region's nucleotide sequence