Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10330 and CA265_RS10335 are separated by 551 nucleotides CA265_RS10335 and CA265_RS10340 overlap by 4 nucleotides CA265_RS10340 and CA265_RS10345 are separated by 67 nucleotides
CA265_RS10330: CA265_RS10330 - hypothetical protein, at 2,554,541 to 2,555,812
_RS10330
CA265_RS10335: CA265_RS10335 - hypothetical protein, at 2,556,364 to 2,558,040
_RS10335
CA265_RS10340: CA265_RS10340 - hypothetical protein, at 2,558,037 to 2,558,627
_RS10340
CA265_RS10345: CA265_RS10345 - hypothetical protein, at 2,558,695 to 2,560,563
_RS10345
Position (kb)
2556
2557
2558
2559 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2555.465 kb on - strand, within CA265_RS10330 at 2555.477 kb on + strand, within CA265_RS10330 at 2555.512 kb on - strand, within CA265_RS10330 at 2555.594 kb on + strand, within CA265_RS10330 at 2555.595 kb on - strand, within CA265_RS10330 at 2555.634 kb on + strand, within CA265_RS10330 at 2555.686 kb on - strand at 2555.696 kb on - strand at 2555.710 kb on - strand at 2555.849 kb on - strand at 2555.896 kb on + strand at 2555.897 kb on - strand at 2555.897 kb on - strand at 2555.897 kb on - strand at 2555.923 kb on - strand at 2555.928 kb on + strand at 2555.929 kb on - strand at 2556.000 kb on + strand at 2556.101 kb on + strand at 2556.102 kb on - strand at 2556.153 kb on + strand at 2556.166 kb on - strand at 2556.252 kb on + strand at 2556.264 kb on + strand at 2556.505 kb on - strand at 2556.534 kb on + strand, within CA265_RS10335 at 2556.534 kb on + strand at 2556.558 kb on + strand, within CA265_RS10335 at 2556.558 kb on + strand, within CA265_RS10335 at 2556.559 kb on - strand, within CA265_RS10335 at 2556.689 kb on - strand, within CA265_RS10335 at 2556.795 kb on + strand, within CA265_RS10335 at 2556.828 kb on - strand, within CA265_RS10335 at 2556.849 kb on - strand, within CA265_RS10335 at 2556.876 kb on + strand, within CA265_RS10335 at 2556.931 kb on - strand, within CA265_RS10335 at 2556.933 kb on - strand, within CA265_RS10335 at 2556.933 kb on - strand, within CA265_RS10335 at 2557.119 kb on + strand, within CA265_RS10335 at 2557.120 kb on - strand, within CA265_RS10335 at 2557.120 kb on - strand, within CA265_RS10335 at 2557.120 kb on - strand, within CA265_RS10335 at 2557.180 kb on + strand, within CA265_RS10335 at 2557.180 kb on + strand, within CA265_RS10335 at 2557.213 kb on - strand, within CA265_RS10335 at 2557.243 kb on + strand, within CA265_RS10335 at 2557.463 kb on + strand, within CA265_RS10335 at 2557.527 kb on - strand, within CA265_RS10335 at 2557.609 kb on - strand, within CA265_RS10335 at 2557.609 kb on - strand, within CA265_RS10335 at 2557.609 kb on - strand, within CA265_RS10335 at 2557.609 kb on - strand, within CA265_RS10335 at 2557.609 kb on - strand, within CA265_RS10335 at 2557.796 kb on - strand, within CA265_RS10335 at 2557.824 kb on - strand, within CA265_RS10335 at 2557.824 kb on - strand, within CA265_RS10335 at 2558.029 kb on - strand at 2558.031 kb on - strand at 2558.034 kb on + strand at 2558.034 kb on + strand at 2558.035 kb on - strand at 2558.035 kb on - strand at 2558.035 kb on - strand at 2558.035 kb on - strand at 2558.136 kb on - strand, within CA265_RS10340 at 2558.136 kb on - strand, within CA265_RS10340 at 2558.266 kb on + strand, within CA265_RS10340 at 2558.275 kb on - strand, within CA265_RS10340 at 2558.347 kb on + strand, within CA265_RS10340 at 2558.351 kb on + strand, within CA265_RS10340 at 2558.528 kb on + strand, within CA265_RS10340 at 2558.549 kb on + strand, within CA265_RS10340 at 2558.577 kb on - strand at 2558.638 kb on - strand at 2558.687 kb on + strand at 2558.691 kb on + strand at 2558.691 kb on + strand at 2558.692 kb on - strand at 2558.692 kb on - strand at 2558.704 kb on + strand at 2558.704 kb on + strand at 2558.705 kb on - strand at 2558.801 kb on - strand at 2558.803 kb on - strand at 2558.805 kb on - strand at 2558.844 kb on + strand at 2558.956 kb on - strand, within CA265_RS10345 at 2558.969 kb on + strand, within CA265_RS10345 at 2558.979 kb on + strand, within CA265_RS10345 at 2558.980 kb on - strand, within CA265_RS10345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 2,555,465 - CA265_RS10330 0.73 -0.3 2,555,477 + CA265_RS10330 0.74 -0.4 2,555,512 - CA265_RS10330 0.76 +1.6 2,555,594 + CA265_RS10330 0.83 -0.2 2,555,595 - CA265_RS10330 0.83 +0.5 2,555,634 + CA265_RS10330 0.86 -0.6 2,555,686 - +1.3 2,555,696 - -0.2 2,555,710 - +0.1 2,555,849 - -0.6 2,555,896 + +0.7 2,555,897 - +0.2 2,555,897 - -2.6 2,555,897 - +0.2 2,555,923 - -0.2 2,555,928 + +0.8 2,555,929 - -2.1 2,556,000 + -2.1 2,556,101 + -0.8 2,556,102 - -2.9 2,556,153 + -0.3 2,556,166 - -2.0 2,556,252 + -3.0 2,556,264 + -0.3 2,556,505 - -0.6 2,556,534 + CA265_RS10335 0.10 +0.7 2,556,534 + +0.7 2,556,558 + CA265_RS10335 0.12 -0.6 2,556,558 + CA265_RS10335 0.12 -0.3 2,556,559 - CA265_RS10335 0.12 -0.3 2,556,689 - CA265_RS10335 0.19 -1.4 2,556,795 + CA265_RS10335 0.26 -0.3 2,556,828 - CA265_RS10335 0.28 +0.6 2,556,849 - CA265_RS10335 0.29 -1.1 2,556,876 + CA265_RS10335 0.31 +1.2 2,556,931 - CA265_RS10335 0.34 -1.5 2,556,933 - CA265_RS10335 0.34 -0.2 2,556,933 - CA265_RS10335 0.34 -1.3 2,557,119 + CA265_RS10335 0.45 -1.0 2,557,120 - CA265_RS10335 0.45 -3.7 2,557,120 - CA265_RS10335 0.45 -0.0 2,557,120 - CA265_RS10335 0.45 -0.5 2,557,180 + CA265_RS10335 0.49 -2.1 2,557,180 + CA265_RS10335 0.49 -1.5 2,557,213 - CA265_RS10335 0.51 +0.8 2,557,243 + CA265_RS10335 0.52 -0.0 2,557,463 + CA265_RS10335 0.66 +1.1 2,557,527 - CA265_RS10335 0.69 +0.4 2,557,609 - CA265_RS10335 0.74 -2.0 2,557,609 - CA265_RS10335 0.74 -1.2 2,557,609 - CA265_RS10335 0.74 -3.1 2,557,609 - CA265_RS10335 0.74 -0.4 2,557,609 - CA265_RS10335 0.74 -3.4 2,557,796 - CA265_RS10335 0.85 -0.5 2,557,824 - CA265_RS10335 0.87 -1.8 2,557,824 - CA265_RS10335 0.87 +0.4 2,558,029 - +0.7 2,558,031 - +1.2 2,558,034 + +1.0 2,558,034 + -3.6 2,558,035 - +1.6 2,558,035 - +0.2 2,558,035 - +0.3 2,558,035 - +0.5 2,558,136 - CA265_RS10340 0.17 -1.8 2,558,136 - CA265_RS10340 0.17 -0.2 2,558,266 + CA265_RS10340 0.39 +0.3 2,558,275 - CA265_RS10340 0.40 -2.0 2,558,347 + CA265_RS10340 0.52 +0.3 2,558,351 + CA265_RS10340 0.53 +0.6 2,558,528 + CA265_RS10340 0.83 -1.6 2,558,549 + CA265_RS10340 0.87 -1.1 2,558,577 - +0.3 2,558,638 - +1.7 2,558,687 + -2.2 2,558,691 + -0.2 2,558,691 + +0.0 2,558,692 - +0.5 2,558,692 - +1.3 2,558,704 + -0.2 2,558,704 + +0.3 2,558,705 - +1.1 2,558,801 - +0.2 2,558,803 - -1.0 2,558,805 - -0.9 2,558,844 + +0.1 2,558,956 - CA265_RS10345 0.14 +0.3 2,558,969 + CA265_RS10345 0.15 +0.3 2,558,979 + CA265_RS10345 0.15 +0.5 2,558,980 - CA265_RS10345 0.15 +0.5
Or see this region's nucleotide sequence