Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06235 and CA265_RS06240 are separated by 130 nucleotides CA265_RS06240 and CA265_RS06245 are separated by 136 nucleotides CA265_RS06245 and CA265_RS06250 are separated by 203 nucleotides CA265_RS06250 and CA265_RS06255 are separated by 6 nucleotides
CA265_RS06235: CA265_RS06235 - hypothetical protein, at 1,536,621 to 1,536,842
_RS06235
CA265_RS06240: CA265_RS06240 - hypothetical protein, at 1,536,973 to 1,537,191
_RS06240
CA265_RS06245: CA265_RS06245 - hypothetical protein, at 1,537,328 to 1,537,546
_RS06245
CA265_RS06250: CA265_RS06250 - RNA polymerase subunit sigma-70, at 1,537,750 to 1,538,316
_RS06250
CA265_RS06255: CA265_RS06255 - serine protease, at 1,538,323 to 1,539,417
_RS06255
Position (kb)
1537
1538
1539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1536.858 kb on - strand at 1536.882 kb on + strand at 1536.959 kb on - strand at 1536.964 kb on + strand at 1536.970 kb on - strand at 1536.974 kb on + strand at 1536.975 kb on - strand at 1536.982 kb on - strand at 1537.010 kb on - strand, within CA265_RS06240 at 1537.015 kb on - strand, within CA265_RS06240 at 1537.015 kb on - strand, within CA265_RS06240 at 1537.044 kb on + strand, within CA265_RS06240 at 1537.062 kb on + strand, within CA265_RS06240 at 1537.099 kb on - strand, within CA265_RS06240 at 1537.129 kb on - strand, within CA265_RS06240 at 1537.129 kb on - strand, within CA265_RS06240 at 1537.318 kb on + strand at 1537.318 kb on + strand at 1537.725 kb on + strand at 1537.741 kb on + strand at 1537.808 kb on + strand, within CA265_RS06250 at 1537.849 kb on + strand, within CA265_RS06250 at 1538.058 kb on + strand, within CA265_RS06250 at 1538.241 kb on + strand, within CA265_RS06250 at 1538.342 kb on + strand at 1538.412 kb on - strand at 1538.454 kb on + strand, within CA265_RS06255 at 1538.455 kb on - strand, within CA265_RS06255 at 1538.520 kb on + strand, within CA265_RS06255 at 1538.521 kb on - strand, within CA265_RS06255 at 1538.605 kb on - strand, within CA265_RS06255 at 1538.664 kb on + strand, within CA265_RS06255 at 1538.994 kb on - strand, within CA265_RS06255 at 1539.008 kb on - strand, within CA265_RS06255 at 1539.036 kb on - strand, within CA265_RS06255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 1,536,858 - -1.8 1,536,882 + -0.5 1,536,959 - -1.5 1,536,964 + -3.0 1,536,970 - +1.2 1,536,974 + -1.0 1,536,975 - +1.7 1,536,982 - -1.6 1,537,010 - CA265_RS06240 0.17 -0.2 1,537,015 - CA265_RS06240 0.19 +0.0 1,537,015 - CA265_RS06240 0.19 -1.2 1,537,044 + CA265_RS06240 0.32 -0.9 1,537,062 + CA265_RS06240 0.41 +1.5 1,537,099 - CA265_RS06240 0.58 +1.4 1,537,129 - CA265_RS06240 0.71 -0.0 1,537,129 - CA265_RS06240 0.71 +0.5 1,537,318 + -0.3 1,537,318 + -0.4 1,537,725 + -0.8 1,537,741 + -2.0 1,537,808 + CA265_RS06250 0.10 +2.3 1,537,849 + CA265_RS06250 0.17 +2.2 1,538,058 + CA265_RS06250 0.54 -2.6 1,538,241 + CA265_RS06250 0.87 +0.3 1,538,342 + +1.8 1,538,412 - +0.2 1,538,454 + CA265_RS06255 0.12 -0.8 1,538,455 - CA265_RS06255 0.12 -0.4 1,538,520 + CA265_RS06255 0.18 -0.8 1,538,521 - CA265_RS06255 0.18 +1.3 1,538,605 - CA265_RS06255 0.26 -1.2 1,538,664 + CA265_RS06255 0.31 -0.8 1,538,994 - CA265_RS06255 0.61 -1.0 1,539,008 - CA265_RS06255 0.63 -2.8 1,539,036 - CA265_RS06255 0.65 -0.8
Or see this region's nucleotide sequence