Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04320 and CA265_RS04325 are separated by 73 nucleotides CA265_RS04325 and CA265_RS04330 are separated by 108 nucleotides CA265_RS04330 and CA265_RS04335 are separated by 2 nucleotides CA265_RS04335 and CA265_RS04340 overlap by 8 nucleotides CA265_RS04340 and CA265_RS04345 are separated by 37 nucleotides
CA265_RS04320: CA265_RS04320 - hypothetical protein, at 1,041,848 to 1,043,416
_RS04320
CA265_RS04325: CA265_RS04325 - hypothetical protein, at 1,043,490 to 1,043,681
_RS04325
CA265_RS04330: CA265_RS04330 - transcriptional regulator, at 1,043,790 to 1,044,086
_RS04330
CA265_RS04335: CA265_RS04335 - hypothetical protein, at 1,044,089 to 1,044,724
_RS04335
CA265_RS04340: CA265_RS04340 - hypothetical protein, at 1,044,717 to 1,044,908
_RS04340
CA265_RS04345: CA265_RS04345 - lipoprotein-releasing system ATP-binding protein LolD, at 1,044,946 to 1,045,596
_RS04345
Position (kb)
1043
1044
1045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1042.796 kb on + strand, within CA265_RS04320 at 1042.797 kb on - strand, within CA265_RS04320 at 1042.920 kb on + strand, within CA265_RS04320 at 1042.921 kb on - strand, within CA265_RS04320 at 1042.999 kb on + strand, within CA265_RS04320 at 1042.999 kb on + strand, within CA265_RS04320 at 1043.000 kb on - strand, within CA265_RS04320 at 1043.000 kb on - strand, within CA265_RS04320 at 1043.051 kb on + strand, within CA265_RS04320 at 1043.157 kb on - strand, within CA265_RS04320 at 1043.157 kb on - strand, within CA265_RS04320 at 1043.175 kb on + strand, within CA265_RS04320 at 1043.190 kb on - strand, within CA265_RS04320 at 1043.237 kb on + strand, within CA265_RS04320 at 1043.258 kb on - strand, within CA265_RS04320 at 1043.266 kb on + strand at 1043.285 kb on - strand at 1043.285 kb on - strand at 1043.304 kb on + strand at 1043.305 kb on - strand at 1043.305 kb on - strand at 1043.311 kb on + strand at 1043.334 kb on - strand at 1043.393 kb on + strand at 1043.394 kb on - strand at 1043.465 kb on - strand at 1043.468 kb on - strand at 1043.610 kb on - strand, within CA265_RS04325 at 1043.648 kb on + strand, within CA265_RS04325 at 1043.654 kb on + strand, within CA265_RS04325 at 1043.657 kb on + strand, within CA265_RS04325 at 1043.668 kb on + strand at 1043.680 kb on - strand at 1043.699 kb on + strand at 1043.891 kb on + strand, within CA265_RS04330 at 1043.904 kb on + strand, within CA265_RS04330 at 1043.913 kb on - strand, within CA265_RS04330 at 1043.913 kb on - strand, within CA265_RS04330 at 1043.929 kb on - strand, within CA265_RS04330 at 1043.933 kb on + strand, within CA265_RS04330 at 1043.937 kb on + strand, within CA265_RS04330 at 1043.964 kb on + strand, within CA265_RS04330 at 1044.027 kb on + strand, within CA265_RS04330 at 1044.034 kb on - strand, within CA265_RS04330 at 1044.034 kb on - strand, within CA265_RS04330 at 1044.144 kb on - strand at 1044.145 kb on + strand at 1044.145 kb on + strand at 1044.146 kb on - strand at 1044.168 kb on - strand, within CA265_RS04335 at 1044.209 kb on + strand, within CA265_RS04335 at 1044.209 kb on + strand, within CA265_RS04335 at 1044.209 kb on + strand, within CA265_RS04335 at 1044.209 kb on + strand, within CA265_RS04335 at 1044.210 kb on - strand, within CA265_RS04335 at 1044.210 kb on - strand, within CA265_RS04335 at 1044.212 kb on + strand, within CA265_RS04335 at 1044.213 kb on - strand, within CA265_RS04335 at 1044.247 kb on - strand, within CA265_RS04335 at 1044.266 kb on - strand, within CA265_RS04335 at 1044.266 kb on - strand, within CA265_RS04335 at 1044.290 kb on + strand, within CA265_RS04335 at 1044.310 kb on + strand, within CA265_RS04335 at 1044.311 kb on - strand, within CA265_RS04335 at 1044.315 kb on + strand, within CA265_RS04335 at 1044.316 kb on - strand, within CA265_RS04335 at 1044.316 kb on - strand, within CA265_RS04335 at 1044.349 kb on + strand, within CA265_RS04335 at 1044.353 kb on + strand, within CA265_RS04335 at 1044.380 kb on + strand, within CA265_RS04335 at 1044.380 kb on + strand, within CA265_RS04335 at 1044.385 kb on + strand, within CA265_RS04335 at 1044.452 kb on - strand, within CA265_RS04335 at 1044.493 kb on + strand, within CA265_RS04335 at 1044.613 kb on + strand, within CA265_RS04335 at 1044.613 kb on + strand, within CA265_RS04335 at 1044.613 kb on + strand, within CA265_RS04335 at 1044.614 kb on - strand, within CA265_RS04335 at 1044.636 kb on - strand, within CA265_RS04335 at 1044.636 kb on - strand at 1044.636 kb on - strand, within CA265_RS04335 at 1044.766 kb on + strand, within CA265_RS04340 at 1044.856 kb on + strand, within CA265_RS04340 at 1044.858 kb on + strand, within CA265_RS04340 at 1044.858 kb on + strand, within CA265_RS04340 at 1044.858 kb on + strand, within CA265_RS04340 at 1044.859 kb on - strand, within CA265_RS04340 at 1044.860 kb on + strand, within CA265_RS04340 at 1044.888 kb on + strand, within CA265_RS04340 at 1044.900 kb on + strand at 1044.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 1,042,796 + CA265_RS04320 0.60 -1.4 1,042,797 - CA265_RS04320 0.60 -0.0 1,042,920 + CA265_RS04320 0.68 +1.3 1,042,921 - CA265_RS04320 0.68 +0.4 1,042,999 + CA265_RS04320 0.73 +1.0 1,042,999 + CA265_RS04320 0.73 +0.9 1,043,000 - CA265_RS04320 0.73 -2.0 1,043,000 - CA265_RS04320 0.73 +0.4 1,043,051 + CA265_RS04320 0.77 +2.1 1,043,157 - CA265_RS04320 0.83 +0.2 1,043,157 - CA265_RS04320 0.83 +0.5 1,043,175 + CA265_RS04320 0.85 -1.1 1,043,190 - CA265_RS04320 0.86 +0.8 1,043,237 + CA265_RS04320 0.89 +0.3 1,043,258 - CA265_RS04320 0.90 +0.4 1,043,266 + -1.3 1,043,285 - +0.8 1,043,285 - -1.2 1,043,304 + -1.7 1,043,305 - +1.7 1,043,305 - -1.4 1,043,311 + -3.3 1,043,334 - -0.4 1,043,393 + +1.7 1,043,394 - +0.7 1,043,465 - +1.5 1,043,468 - +1.6 1,043,610 - CA265_RS04325 0.62 +1.5 1,043,648 + CA265_RS04325 0.82 +0.7 1,043,654 + CA265_RS04325 0.85 -0.4 1,043,657 + CA265_RS04325 0.87 -0.8 1,043,668 + +0.9 1,043,680 - +1.1 1,043,699 + -0.5 1,043,891 + CA265_RS04330 0.34 +0.9 1,043,904 + CA265_RS04330 0.38 +1.2 1,043,913 - CA265_RS04330 0.41 +0.0 1,043,913 - CA265_RS04330 0.41 +1.6 1,043,929 - CA265_RS04330 0.47 +2.2 1,043,933 + CA265_RS04330 0.48 +0.1 1,043,937 + CA265_RS04330 0.49 -0.4 1,043,964 + CA265_RS04330 0.59 +0.7 1,044,027 + CA265_RS04330 0.80 +1.6 1,044,034 - CA265_RS04330 0.82 +1.4 1,044,034 - CA265_RS04330 0.82 +0.6 1,044,144 - +0.4 1,044,145 + +1.5 1,044,145 + +3.3 1,044,146 - +0.9 1,044,168 - CA265_RS04335 0.12 +1.2 1,044,209 + CA265_RS04335 0.19 -1.4 1,044,209 + CA265_RS04335 0.19 +1.2 1,044,209 + CA265_RS04335 0.19 +0.2 1,044,209 + CA265_RS04335 0.19 +0.6 1,044,210 - CA265_RS04335 0.19 +0.1 1,044,210 - CA265_RS04335 0.19 +0.5 1,044,212 + CA265_RS04335 0.19 +1.7 1,044,213 - CA265_RS04335 0.19 -1.6 1,044,247 - CA265_RS04335 0.25 -0.1 1,044,266 - CA265_RS04335 0.28 +0.5 1,044,266 - CA265_RS04335 0.28 +0.7 1,044,290 + CA265_RS04335 0.32 +0.7 1,044,310 + CA265_RS04335 0.35 -0.1 1,044,311 - CA265_RS04335 0.35 -0.5 1,044,315 + CA265_RS04335 0.36 -0.9 1,044,316 - CA265_RS04335 0.36 -0.3 1,044,316 - CA265_RS04335 0.36 +0.6 1,044,349 + CA265_RS04335 0.41 -0.9 1,044,353 + CA265_RS04335 0.42 +1.8 1,044,380 + CA265_RS04335 0.46 +0.5 1,044,380 + CA265_RS04335 0.46 +0.3 1,044,385 + CA265_RS04335 0.47 +0.3 1,044,452 - CA265_RS04335 0.57 -2.2 1,044,493 + CA265_RS04335 0.64 -0.6 1,044,613 + CA265_RS04335 0.82 +1.3 1,044,613 + CA265_RS04335 0.82 -0.1 1,044,613 + CA265_RS04335 0.82 +0.0 1,044,614 - CA265_RS04335 0.83 -0.9 1,044,636 - CA265_RS04335 0.86 +0.0 1,044,636 - +1.9 1,044,636 - CA265_RS04335 0.86 +0.7 1,044,766 + CA265_RS04340 0.26 +0.1 1,044,856 + CA265_RS04340 0.72 +1.5 1,044,858 + CA265_RS04340 0.73 +0.1 1,044,858 + CA265_RS04340 0.73 -3.3 1,044,858 + CA265_RS04340 0.73 -2.5 1,044,859 - CA265_RS04340 0.74 -2.4 1,044,860 + CA265_RS04340 0.74 +0.1 1,044,888 + CA265_RS04340 0.89 -0.6 1,044,900 + +1.6 1,044,911 - -0.1
Or see this region's nucleotide sequence