Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04165 and CA265_RS04170 are separated by 390 nucleotides CA265_RS04170 and CA265_RS04175 are separated by 122 nucleotides
CA265_RS04165: CA265_RS04165 - sigma-54-dependent Fis family transcriptional regulator, at 1,005,491 to 1,006,861
_RS04165
CA265_RS04170: CA265_RS04170 - transporter, at 1,007,252 to 1,008,496
_RS04170
CA265_RS04175: CA265_RS04175 - phosphonate ABC transporter ATP-binding protein, at 1,008,619 to 1,009,317
_RS04175
Position (kb)
1007
1008
1009 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1006.334 kb on - strand, within CA265_RS04165 at 1006.445 kb on + strand, within CA265_RS04165 at 1006.564 kb on + strand, within CA265_RS04165 at 1006.607 kb on + strand, within CA265_RS04165 at 1006.616 kb on + strand, within CA265_RS04165 at 1006.617 kb on - strand, within CA265_RS04165 at 1006.620 kb on + strand, within CA265_RS04165 at 1006.628 kb on - strand, within CA265_RS04165 at 1006.656 kb on + strand, within CA265_RS04165 at 1006.693 kb on - strand, within CA265_RS04165 at 1006.768 kb on + strand at 1006.782 kb on - strand at 1006.862 kb on - strand at 1006.888 kb on - strand at 1006.889 kb on + strand at 1006.915 kb on - strand at 1006.941 kb on - strand at 1006.988 kb on + strand at 1007.002 kb on + strand at 1007.016 kb on + strand at 1007.265 kb on + strand at 1007.287 kb on + strand at 1007.288 kb on - strand at 1007.299 kb on - strand at 1007.299 kb on - strand at 1007.452 kb on - strand, within CA265_RS04170 at 1007.452 kb on - strand, within CA265_RS04170 at 1007.475 kb on + strand, within CA265_RS04170 at 1007.683 kb on - strand, within CA265_RS04170 at 1007.683 kb on - strand, within CA265_RS04170 at 1007.731 kb on - strand, within CA265_RS04170 at 1007.734 kb on + strand, within CA265_RS04170 at 1007.735 kb on - strand, within CA265_RS04170 at 1007.812 kb on + strand, within CA265_RS04170 at 1007.860 kb on + strand, within CA265_RS04170 at 1008.006 kb on - strand, within CA265_RS04170 at 1008.006 kb on - strand, within CA265_RS04170 at 1008.006 kb on - strand, within CA265_RS04170 at 1008.051 kb on - strand, within CA265_RS04170 at 1008.052 kb on + strand, within CA265_RS04170 at 1008.078 kb on - strand, within CA265_RS04170 at 1008.082 kb on + strand, within CA265_RS04170 at 1008.140 kb on - strand, within CA265_RS04170 at 1008.155 kb on - strand, within CA265_RS04170 at 1008.159 kb on + strand, within CA265_RS04170 at 1008.317 kb on - strand, within CA265_RS04170 at 1008.398 kb on + strand at 1008.399 kb on - strand at 1008.399 kb on - strand at 1008.490 kb on + strand at 1008.490 kb on + strand at 1008.491 kb on - strand at 1008.494 kb on + strand at 1008.502 kb on + strand at 1008.517 kb on - strand at 1008.552 kb on - strand at 1008.565 kb on - strand at 1008.608 kb on + strand at 1008.803 kb on - strand, within CA265_RS04175 at 1009.008 kb on + strand, within CA265_RS04175 at 1009.067 kb on + strand, within CA265_RS04175 at 1009.138 kb on - strand, within CA265_RS04175 at 1009.150 kb on - strand, within CA265_RS04175 at 1009.157 kb on + strand, within CA265_RS04175 at 1009.157 kb on + strand, within CA265_RS04175 at 1009.158 kb on - strand, within CA265_RS04175 at 1009.179 kb on - strand, within CA265_RS04175 at 1009.312 kb on - strand at 1009.316 kb on - strand at 1009.412 kb on + strand at 1009.413 kb on - strand at 1009.413 kb on - strand at 1009.413 kb on - strand at 1009.419 kb on + strand at 1009.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 1,006,334 - CA265_RS04165 0.61 -2.3 1,006,445 + CA265_RS04165 0.70 +0.6 1,006,564 + CA265_RS04165 0.78 +1.0 1,006,607 + CA265_RS04165 0.81 +0.5 1,006,616 + CA265_RS04165 0.82 +0.2 1,006,617 - CA265_RS04165 0.82 -0.5 1,006,620 + CA265_RS04165 0.82 -2.8 1,006,628 - CA265_RS04165 0.83 -1.4 1,006,656 + CA265_RS04165 0.85 -0.5 1,006,693 - CA265_RS04165 0.88 -1.6 1,006,768 + +0.1 1,006,782 - -1.9 1,006,862 - -2.2 1,006,888 - +0.9 1,006,889 + +0.6 1,006,915 - -0.2 1,006,941 - +2.2 1,006,988 + -2.5 1,007,002 + +0.7 1,007,016 + +0.4 1,007,265 + -0.2 1,007,287 + -1.1 1,007,288 - -1.0 1,007,299 - -1.0 1,007,299 - +1.2 1,007,452 - CA265_RS04170 0.16 -1.2 1,007,452 - CA265_RS04170 0.16 +1.7 1,007,475 + CA265_RS04170 0.18 +0.7 1,007,683 - CA265_RS04170 0.35 +0.1 1,007,683 - CA265_RS04170 0.35 +0.5 1,007,731 - CA265_RS04170 0.38 +1.9 1,007,734 + CA265_RS04170 0.39 -1.6 1,007,735 - CA265_RS04170 0.39 -0.5 1,007,812 + CA265_RS04170 0.45 -0.1 1,007,860 + CA265_RS04170 0.49 -2.1 1,008,006 - CA265_RS04170 0.61 -0.1 1,008,006 - CA265_RS04170 0.61 -0.8 1,008,006 - CA265_RS04170 0.61 +0.3 1,008,051 - CA265_RS04170 0.64 -0.6 1,008,052 + CA265_RS04170 0.64 -0.9 1,008,078 - CA265_RS04170 0.66 +0.0 1,008,082 + CA265_RS04170 0.67 +0.9 1,008,140 - CA265_RS04170 0.71 +0.8 1,008,155 - CA265_RS04170 0.73 -0.2 1,008,159 + CA265_RS04170 0.73 +0.7 1,008,317 - CA265_RS04170 0.86 -0.6 1,008,398 + -2.1 1,008,399 - +0.7 1,008,399 - +0.9 1,008,490 + +0.9 1,008,490 + +0.5 1,008,491 - -1.6 1,008,494 + +0.5 1,008,502 + +0.4 1,008,517 - +0.9 1,008,552 - +0.3 1,008,565 - +1.1 1,008,608 + -1.9 1,008,803 - CA265_RS04175 0.26 +0.4 1,009,008 + CA265_RS04175 0.56 +2.2 1,009,067 + CA265_RS04175 0.64 +0.1 1,009,138 - CA265_RS04175 0.74 +0.7 1,009,150 - CA265_RS04175 0.76 -0.4 1,009,157 + CA265_RS04175 0.77 +0.1 1,009,157 + CA265_RS04175 0.77 -0.3 1,009,158 - CA265_RS04175 0.77 -3.7 1,009,179 - CA265_RS04175 0.80 -0.7 1,009,312 - +1.1 1,009,316 - +0.6 1,009,412 + -0.6 1,009,413 - -1.6 1,009,413 - -0.2 1,009,413 - -1.3 1,009,419 + +0.4 1,009,471 - -3.8
Or see this region's nucleotide sequence