Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04020 and CA265_RS04025 are separated by 275 nucleotides CA265_RS04025 and CA265_RS04030 are separated by 87 nucleotides
CA265_RS04020: CA265_RS04020 - hypothetical protein, at 961,941 to 963,233
_RS04020
CA265_RS04025: CA265_RS04025 - glycoside hydrolase family 2, at 963,509 to 966,673
_RS04025
CA265_RS04030: CA265_RS04030 - alpha-galactosidase, at 966,761 to 967,960
_RS04030
Position (kb) 963
964
965
966
967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 962.605 kb on - strand, within CA265_RS04020 at 962.607 kb on - strand, within CA265_RS04020 at 962.625 kb on + strand, within CA265_RS04020 at 962.626 kb on - strand, within CA265_RS04020 at 962.637 kb on - strand, within CA265_RS04020 at 962.671 kb on + strand, within CA265_RS04020 at 962.726 kb on + strand, within CA265_RS04020 at 962.727 kb on - strand, within CA265_RS04020 at 962.772 kb on - strand, within CA265_RS04020 at 962.772 kb on - strand, within CA265_RS04020 at 962.774 kb on - strand, within CA265_RS04020 at 962.838 kb on - strand, within CA265_RS04020 at 962.873 kb on - strand, within CA265_RS04020 at 962.935 kb on - strand, within CA265_RS04020 at 962.948 kb on + strand, within CA265_RS04020 at 963.042 kb on + strand, within CA265_RS04020 at 963.043 kb on - strand, within CA265_RS04020 at 963.080 kb on - strand, within CA265_RS04020 at 963.263 kb on + strand at 963.264 kb on - strand at 963.465 kb on - strand at 963.465 kb on - strand at 963.487 kb on + strand at 963.498 kb on - strand at 963.500 kb on - strand at 963.543 kb on + strand at 963.670 kb on + strand at 963.757 kb on + strand at 963.830 kb on - strand, within CA265_RS04025 at 963.855 kb on + strand, within CA265_RS04025 at 963.888 kb on + strand, within CA265_RS04025 at 963.931 kb on + strand, within CA265_RS04025 at 963.931 kb on + strand, within CA265_RS04025 at 963.932 kb on - strand, within CA265_RS04025 at 964.008 kb on + strand, within CA265_RS04025 at 964.008 kb on + strand, within CA265_RS04025 at 964.009 kb on - strand, within CA265_RS04025 at 964.063 kb on + strand, within CA265_RS04025 at 964.063 kb on + strand, within CA265_RS04025 at 964.064 kb on - strand, within CA265_RS04025 at 964.073 kb on - strand, within CA265_RS04025 at 964.076 kb on - strand, within CA265_RS04025 at 964.076 kb on - strand, within CA265_RS04025 at 964.107 kb on - strand, within CA265_RS04025 at 964.145 kb on + strand, within CA265_RS04025 at 964.146 kb on - strand, within CA265_RS04025 at 964.146 kb on - strand, within CA265_RS04025 at 964.167 kb on - strand, within CA265_RS04025 at 964.204 kb on - strand, within CA265_RS04025 at 964.204 kb on - strand, within CA265_RS04025 at 964.218 kb on - strand, within CA265_RS04025 at 964.221 kb on + strand, within CA265_RS04025 at 964.243 kb on + strand, within CA265_RS04025 at 964.285 kb on + strand, within CA265_RS04025 at 964.286 kb on - strand, within CA265_RS04025 at 964.306 kb on - strand, within CA265_RS04025 at 964.375 kb on - strand, within CA265_RS04025 at 964.444 kb on - strand, within CA265_RS04025 at 964.451 kb on + strand, within CA265_RS04025 at 964.452 kb on - strand, within CA265_RS04025 at 964.486 kb on + strand, within CA265_RS04025 at 964.521 kb on + strand, within CA265_RS04025 at 964.521 kb on + strand, within CA265_RS04025 at 964.522 kb on - strand, within CA265_RS04025 at 964.532 kb on + strand, within CA265_RS04025 at 964.533 kb on - strand, within CA265_RS04025 at 964.563 kb on + strand, within CA265_RS04025 at 964.660 kb on - strand, within CA265_RS04025 at 964.675 kb on + strand, within CA265_RS04025 at 964.693 kb on + strand, within CA265_RS04025 at 964.694 kb on - strand, within CA265_RS04025 at 964.694 kb on - strand, within CA265_RS04025 at 964.749 kb on + strand, within CA265_RS04025 at 964.750 kb on - strand, within CA265_RS04025 at 964.750 kb on - strand, within CA265_RS04025 at 964.859 kb on + strand, within CA265_RS04025 at 964.859 kb on + strand, within CA265_RS04025 at 964.862 kb on + strand, within CA265_RS04025 at 964.892 kb on - strand, within CA265_RS04025 at 964.981 kb on + strand, within CA265_RS04025 at 965.019 kb on - strand, within CA265_RS04025 at 965.019 kb on - strand, within CA265_RS04025 at 965.167 kb on + strand, within CA265_RS04025 at 965.168 kb on - strand, within CA265_RS04025 at 965.219 kb on + strand, within CA265_RS04025 at 965.220 kb on - strand, within CA265_RS04025 at 965.246 kb on - strand, within CA265_RS04025 at 965.278 kb on - strand, within CA265_RS04025 at 965.369 kb on - strand at 965.402 kb on - strand, within CA265_RS04025 at 965.402 kb on - strand, within CA265_RS04025 at 965.449 kb on - strand, within CA265_RS04025 at 965.456 kb on + strand, within CA265_RS04025 at 965.457 kb on - strand, within CA265_RS04025 at 965.458 kb on + strand, within CA265_RS04025 at 965.462 kb on - strand, within CA265_RS04025 at 965.503 kb on + strand, within CA265_RS04025 at 965.504 kb on - strand, within CA265_RS04025 at 965.532 kb on + strand at 965.532 kb on + strand, within CA265_RS04025 at 965.533 kb on - strand, within CA265_RS04025 at 965.538 kb on + strand, within CA265_RS04025 at 965.539 kb on - strand, within CA265_RS04025 at 965.591 kb on - strand, within CA265_RS04025 at 965.625 kb on + strand, within CA265_RS04025 at 965.626 kb on - strand, within CA265_RS04025 at 965.626 kb on - strand, within CA265_RS04025 at 965.648 kb on + strand, within CA265_RS04025 at 965.723 kb on + strand, within CA265_RS04025 at 965.777 kb on + strand, within CA265_RS04025 at 965.849 kb on - strand, within CA265_RS04025 at 965.888 kb on + strand, within CA265_RS04025 at 965.889 kb on - strand, within CA265_RS04025 at 965.889 kb on - strand, within CA265_RS04025 at 965.988 kb on + strand, within CA265_RS04025 at 965.989 kb on - strand, within CA265_RS04025 at 966.007 kb on - strand, within CA265_RS04025 at 966.016 kb on + strand, within CA265_RS04025 at 966.022 kb on + strand, within CA265_RS04025 at 966.022 kb on + strand, within CA265_RS04025 at 966.023 kb on - strand, within CA265_RS04025 at 966.028 kb on + strand, within CA265_RS04025 at 966.035 kb on - strand, within CA265_RS04025 at 966.044 kb on - strand, within CA265_RS04025 at 966.073 kb on - strand, within CA265_RS04025 at 966.090 kb on + strand, within CA265_RS04025 at 966.091 kb on - strand, within CA265_RS04025 at 966.153 kb on + strand, within CA265_RS04025 at 966.166 kb on + strand, within CA265_RS04025 at 966.167 kb on - strand, within CA265_RS04025 at 966.250 kb on + strand, within CA265_RS04025 at 966.252 kb on - strand, within CA265_RS04025 at 966.300 kb on + strand, within CA265_RS04025 at 966.308 kb on - strand, within CA265_RS04025 at 966.308 kb on - strand, within CA265_RS04025 at 966.314 kb on - strand, within CA265_RS04025 at 966.370 kb on + strand at 966.404 kb on - strand at 966.483 kb on + strand at 966.529 kb on - strand at 966.570 kb on + strand at 966.571 kb on - strand at 966.602 kb on - strand at 966.650 kb on + strand at 966.651 kb on - strand at 966.654 kb on - strand at 966.656 kb on - strand at 966.671 kb on + strand at 966.672 kb on - strand at 966.704 kb on + strand at 966.705 kb on - strand at 966.737 kb on + strand at 966.737 kb on + strand at 966.737 kb on + strand at 966.756 kb on + strand at 966.756 kb on + strand at 966.757 kb on - strand at 966.789 kb on + strand at 966.895 kb on + strand, within CA265_RS04030 at 967.039 kb on - strand, within CA265_RS04030 at 967.088 kb on + strand, within CA265_RS04030 at 967.089 kb on - strand, within CA265_RS04030 at 967.123 kb on + strand, within CA265_RS04030 at 967.184 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.294 kb on + strand, within CA265_RS04030 at 967.295 kb on - strand, within CA265_RS04030 at 967.352 kb on - strand, within CA265_RS04030 at 967.367 kb on + strand, within CA265_RS04030 at 967.368 kb on - strand, within CA265_RS04030 at 967.381 kb on + strand, within CA265_RS04030 at 967.381 kb on - strand, within CA265_RS04030 at 967.417 kb on + strand, within CA265_RS04030 at 967.417 kb on + strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.421 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.538 kb on - strand, within CA265_RS04030 at 967.587 kb on - strand, within CA265_RS04030 at 967.597 kb on + strand, within CA265_RS04030 at 967.598 kb on - strand, within CA265_RS04030 at 967.609 kb on - strand, within CA265_RS04030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 962,605 - CA265_RS04020 0.51 -3.4 962,607 - CA265_RS04020 0.52 +2.2 962,625 + CA265_RS04020 0.53 -0.4 962,626 - CA265_RS04020 0.53 -1.0 962,637 - CA265_RS04020 0.54 +1.0 962,671 + CA265_RS04020 0.56 -0.0 962,726 + CA265_RS04020 0.61 -0.4 962,727 - CA265_RS04020 0.61 +0.7 962,772 - CA265_RS04020 0.64 -0.1 962,772 - CA265_RS04020 0.64 +0.3 962,774 - CA265_RS04020 0.64 -1.4 962,838 - CA265_RS04020 0.69 -1.4 962,873 - CA265_RS04020 0.72 -1.8 962,935 - CA265_RS04020 0.77 -1.0 962,948 + CA265_RS04020 0.78 +0.9 963,042 + CA265_RS04020 0.85 +0.5 963,043 - CA265_RS04020 0.85 +1.3 963,080 - CA265_RS04020 0.88 -1.2 963,263 + +1.0 963,264 - -0.2 963,465 - -1.3 963,465 - +0.6 963,487 + -0.7 963,498 - -1.8 963,500 - -3.1 963,543 + +1.2 963,670 + -2.5 963,757 + +0.3 963,830 - CA265_RS04025 0.10 -0.9 963,855 + CA265_RS04025 0.11 +0.7 963,888 + CA265_RS04025 0.12 +0.3 963,931 + CA265_RS04025 0.13 +1.0 963,931 + CA265_RS04025 0.13 -1.0 963,932 - CA265_RS04025 0.13 -1.2 964,008 + CA265_RS04025 0.16 -0.6 964,008 + CA265_RS04025 0.16 -1.3 964,009 - CA265_RS04025 0.16 +0.2 964,063 + CA265_RS04025 0.18 +0.2 964,063 + CA265_RS04025 0.18 -0.0 964,064 - CA265_RS04025 0.18 -2.0 964,073 - CA265_RS04025 0.18 -3.7 964,076 - CA265_RS04025 0.18 -1.0 964,076 - CA265_RS04025 0.18 -1.2 964,107 - CA265_RS04025 0.19 +0.6 964,145 + CA265_RS04025 0.20 +0.4 964,146 - CA265_RS04025 0.20 +0.5 964,146 - CA265_RS04025 0.20 +0.6 964,167 - CA265_RS04025 0.21 +0.2 964,204 - CA265_RS04025 0.22 -2.0 964,204 - CA265_RS04025 0.22 +0.6 964,218 - CA265_RS04025 0.22 -2.6 964,221 + CA265_RS04025 0.22 +0.5 964,243 + CA265_RS04025 0.23 -0.0 964,285 + CA265_RS04025 0.25 -0.6 964,286 - CA265_RS04025 0.25 +2.2 964,306 - CA265_RS04025 0.25 +0.1 964,375 - CA265_RS04025 0.27 -0.0 964,444 - CA265_RS04025 0.30 -0.8 964,451 + CA265_RS04025 0.30 -0.9 964,452 - CA265_RS04025 0.30 -2.7 964,486 + CA265_RS04025 0.31 -0.1 964,521 + CA265_RS04025 0.32 +0.4 964,521 + CA265_RS04025 0.32 +0.0 964,522 - CA265_RS04025 0.32 -0.6 964,532 + CA265_RS04025 0.32 -0.5 964,533 - CA265_RS04025 0.32 -0.3 964,563 + CA265_RS04025 0.33 +0.6 964,660 - CA265_RS04025 0.36 +0.5 964,675 + CA265_RS04025 0.37 -0.6 964,693 + CA265_RS04025 0.37 -1.1 964,694 - CA265_RS04025 0.37 -1.5 964,694 - CA265_RS04025 0.37 -3.6 964,749 + CA265_RS04025 0.39 +1.0 964,750 - CA265_RS04025 0.39 -0.5 964,750 - CA265_RS04025 0.39 +0.4 964,859 + CA265_RS04025 0.43 -0.1 964,859 + CA265_RS04025 0.43 -2.3 964,862 + CA265_RS04025 0.43 -0.1 964,892 - CA265_RS04025 0.44 +0.4 964,981 + CA265_RS04025 0.47 +1.5 965,019 - CA265_RS04025 0.48 +0.4 965,019 - CA265_RS04025 0.48 -0.4 965,167 + CA265_RS04025 0.52 -2.0 965,168 - CA265_RS04025 0.52 +0.2 965,219 + CA265_RS04025 0.54 +0.1 965,220 - CA265_RS04025 0.54 +0.1 965,246 - CA265_RS04025 0.55 +1.0 965,278 - CA265_RS04025 0.56 -2.9 965,369 - +1.0 965,402 - CA265_RS04025 0.60 +0.4 965,402 - CA265_RS04025 0.60 -1.9 965,449 - CA265_RS04025 0.61 +0.6 965,456 + CA265_RS04025 0.62 -0.4 965,457 - CA265_RS04025 0.62 +0.6 965,458 + CA265_RS04025 0.62 -0.9 965,462 - CA265_RS04025 0.62 +0.7 965,503 + CA265_RS04025 0.63 -0.2 965,504 - CA265_RS04025 0.63 -0.7 965,532 + +1.6 965,532 + CA265_RS04025 0.64 +0.6 965,533 - CA265_RS04025 0.64 +1.2 965,538 + CA265_RS04025 0.64 +0.5 965,539 - CA265_RS04025 0.64 +0.6 965,591 - CA265_RS04025 0.66 +0.2 965,625 + CA265_RS04025 0.67 -0.1 965,626 - CA265_RS04025 0.67 -0.3 965,626 - CA265_RS04025 0.67 -0.8 965,648 + CA265_RS04025 0.68 -0.4 965,723 + CA265_RS04025 0.70 -0.6 965,777 + CA265_RS04025 0.72 -1.0 965,849 - CA265_RS04025 0.74 -2.5 965,888 + CA265_RS04025 0.75 -0.3 965,889 - CA265_RS04025 0.75 +0.3 965,889 - CA265_RS04025 0.75 -2.1 965,988 + CA265_RS04025 0.78 -0.2 965,989 - CA265_RS04025 0.78 -0.4 966,007 - CA265_RS04025 0.79 +0.0 966,016 + CA265_RS04025 0.79 -0.3 966,022 + CA265_RS04025 0.79 +0.6 966,022 + CA265_RS04025 0.79 +0.5 966,023 - CA265_RS04025 0.79 +1.1 966,028 + CA265_RS04025 0.80 +1.1 966,035 - CA265_RS04025 0.80 +0.6 966,044 - CA265_RS04025 0.80 -0.4 966,073 - CA265_RS04025 0.81 +0.3 966,090 + CA265_RS04025 0.82 +0.4 966,091 - CA265_RS04025 0.82 -0.2 966,153 + CA265_RS04025 0.84 +1.8 966,166 + CA265_RS04025 0.84 -1.5 966,167 - CA265_RS04025 0.84 -1.2 966,250 + CA265_RS04025 0.87 -1.1 966,252 - CA265_RS04025 0.87 -1.1 966,300 + CA265_RS04025 0.88 +1.7 966,308 - CA265_RS04025 0.88 -1.0 966,308 - CA265_RS04025 0.88 -0.4 966,314 - CA265_RS04025 0.89 -1.4 966,370 + -1.0 966,404 - -1.6 966,483 + -0.2 966,529 - +1.1 966,570 + +1.8 966,571 - -3.0 966,602 - +1.5 966,650 + -0.5 966,651 - +1.8 966,654 - +1.1 966,656 - +0.2 966,671 + -0.6 966,672 - +1.2 966,704 + -2.1 966,705 - -0.6 966,737 + -0.5 966,737 + +1.1 966,737 + -0.4 966,756 + -0.1 966,756 + -0.4 966,757 - -2.4 966,789 + -3.4 966,895 + CA265_RS04030 0.11 -0.4 967,039 - CA265_RS04030 0.23 +0.4 967,088 + CA265_RS04030 0.27 -1.0 967,089 - CA265_RS04030 0.27 +0.1 967,123 + CA265_RS04030 0.30 -1.2 967,184 + CA265_RS04030 0.35 +0.4 967,282 + CA265_RS04030 0.43 -0.5 967,282 + CA265_RS04030 0.43 -3.0 967,282 + CA265_RS04030 0.43 -1.9 967,282 + CA265_RS04030 0.43 +0.1 967,294 + CA265_RS04030 0.44 +0.2 967,295 - CA265_RS04030 0.45 +0.8 967,352 - CA265_RS04030 0.49 -2.6 967,367 + CA265_RS04030 0.51 +1.5 967,368 - CA265_RS04030 0.51 -0.2 967,381 + CA265_RS04030 0.52 -1.0 967,381 - CA265_RS04030 0.52 -0.2 967,417 + CA265_RS04030 0.55 +0.1 967,417 + CA265_RS04030 0.55 -1.8 967,418 - CA265_RS04030 0.55 -0.2 967,418 - CA265_RS04030 0.55 -0.4 967,418 - CA265_RS04030 0.55 -0.0 967,421 + CA265_RS04030 0.55 -3.6 967,506 + CA265_RS04030 0.62 -1.4 967,506 + CA265_RS04030 0.62 +0.5 967,506 + CA265_RS04030 0.62 -0.3 967,538 - CA265_RS04030 0.65 +0.2 967,587 - CA265_RS04030 0.69 +0.1 967,597 + CA265_RS04030 0.70 -1.1 967,598 - CA265_RS04030 0.70 -0.0 967,609 - CA265_RS04030 0.71 -0.1
Or see this region's nucleotide sequence