Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03425 and CA265_RS03430 overlap by 23 nucleotides CA265_RS03430 and CA265_RS03435 are separated by 1 nucleotides CA265_RS03435 and CA265_RS03440 are separated by 181 nucleotides
CA265_RS03425: CA265_RS03425 - FAD-dependent oxidoreductase, at 828,009 to 829,664
_RS03425
CA265_RS03430: CA265_RS03430 - spermidine synthase, at 829,642 to 831,153
_RS03430
CA265_RS03435: CA265_RS03435 - hypothetical protein, at 831,155 to 831,379
_RS03435
CA265_RS03440: CA265_RS03440 - hypothetical protein, at 831,561 to 832,874
_RS03440
Position (kb) 829
830
831
832 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 828.649 kb on - strand, within CA265_RS03425 at 828.651 kb on - strand, within CA265_RS03425 at 828.684 kb on + strand, within CA265_RS03425 at 828.685 kb on - strand, within CA265_RS03425 at 828.685 kb on - strand, within CA265_RS03425 at 828.765 kb on + strand, within CA265_RS03425 at 828.814 kb on + strand, within CA265_RS03425 at 828.837 kb on + strand, within CA265_RS03425 at 828.842 kb on + strand, within CA265_RS03425 at 828.859 kb on + strand, within CA265_RS03425 at 828.859 kb on + strand, within CA265_RS03425 at 828.877 kb on + strand, within CA265_RS03425 at 828.887 kb on - strand, within CA265_RS03425 at 828.892 kb on - strand, within CA265_RS03425 at 828.899 kb on - strand, within CA265_RS03425 at 829.044 kb on + strand, within CA265_RS03425 at 829.178 kb on - strand, within CA265_RS03425 at 829.214 kb on + strand, within CA265_RS03425 at 829.268 kb on + strand, within CA265_RS03425 at 829.268 kb on + strand, within CA265_RS03425 at 829.298 kb on + strand, within CA265_RS03425 at 829.301 kb on + strand, within CA265_RS03425 at 829.301 kb on + strand, within CA265_RS03425 at 829.302 kb on - strand, within CA265_RS03425 at 829.302 kb on - strand, within CA265_RS03425 at 829.302 kb on - strand, within CA265_RS03425 at 829.434 kb on + strand, within CA265_RS03425 at 829.512 kb on + strand at 829.513 kb on - strand at 829.538 kb on + strand at 829.625 kb on - strand at 829.705 kb on + strand at 829.705 kb on + strand at 829.786 kb on - strand at 829.789 kb on + strand at 829.794 kb on - strand, within CA265_RS03430 at 829.794 kb on - strand, within CA265_RS03430 at 829.836 kb on + strand at 829.837 kb on - strand, within CA265_RS03430 at 829.868 kb on + strand, within CA265_RS03430 at 829.869 kb on - strand, within CA265_RS03430 at 829.957 kb on - strand, within CA265_RS03430 at 829.980 kb on + strand, within CA265_RS03430 at 829.990 kb on + strand, within CA265_RS03430 at 829.990 kb on + strand, within CA265_RS03430 at 829.991 kb on - strand, within CA265_RS03430 at 829.991 kb on - strand, within CA265_RS03430 at 829.991 kb on - strand, within CA265_RS03430 at 829.991 kb on - strand, within CA265_RS03430 at 830.025 kb on - strand, within CA265_RS03430 at 830.027 kb on - strand, within CA265_RS03430 at 830.027 kb on - strand, within CA265_RS03430 at 830.134 kb on + strand, within CA265_RS03430 at 830.134 kb on + strand, within CA265_RS03430 at 830.160 kb on + strand, within CA265_RS03430 at 830.221 kb on + strand, within CA265_RS03430 at 830.222 kb on - strand, within CA265_RS03430 at 830.240 kb on - strand, within CA265_RS03430 at 830.245 kb on - strand, within CA265_RS03430 at 830.249 kb on + strand, within CA265_RS03430 at 830.300 kb on - strand, within CA265_RS03430 at 830.464 kb on + strand, within CA265_RS03430 at 830.464 kb on + strand, within CA265_RS03430 at 830.464 kb on + strand, within CA265_RS03430 at 830.465 kb on - strand, within CA265_RS03430 at 830.465 kb on - strand, within CA265_RS03430 at 830.465 kb on - strand, within CA265_RS03430 at 830.480 kb on + strand, within CA265_RS03430 at 830.481 kb on - strand, within CA265_RS03430 at 830.611 kb on + strand, within CA265_RS03430 at 830.612 kb on - strand, within CA265_RS03430 at 830.612 kb on - strand, within CA265_RS03430 at 830.617 kb on + strand, within CA265_RS03430 at 830.779 kb on - strand, within CA265_RS03430 at 830.844 kb on - strand, within CA265_RS03430 at 830.973 kb on + strand, within CA265_RS03430 at 830.973 kb on + strand, within CA265_RS03430 at 830.989 kb on + strand, within CA265_RS03430 at 830.990 kb on - strand, within CA265_RS03430 at 830.990 kb on - strand, within CA265_RS03430 at 830.997 kb on + strand, within CA265_RS03430 at 831.087 kb on - strand at 831.088 kb on + strand at 831.089 kb on - strand at 831.090 kb on + strand at 831.090 kb on + strand at 831.090 kb on + strand at 831.128 kb on - strand at 831.156 kb on + strand at 831.178 kb on + strand, within CA265_RS03435 at 831.181 kb on + strand, within CA265_RS03435 at 831.199 kb on + strand, within CA265_RS03435 at 831.200 kb on - strand, within CA265_RS03435 at 831.327 kb on + strand, within CA265_RS03435 at 831.344 kb on - strand at 831.348 kb on + strand, within CA265_RS03435 at 831.371 kb on + strand at 831.389 kb on - strand at 831.404 kb on + strand at 831.409 kb on - strand at 831.409 kb on - strand at 831.428 kb on + strand at 831.462 kb on - strand at 831.497 kb on - strand at 831.511 kb on - strand at 831.525 kb on + strand at 831.526 kb on - strand at 831.562 kb on + strand at 831.654 kb on - strand at 831.718 kb on + strand, within CA265_RS03440 at 831.735 kb on + strand, within CA265_RS03440 at 831.755 kb on - strand, within CA265_RS03440 at 831.755 kb on - strand, within CA265_RS03440 at 831.762 kb on + strand, within CA265_RS03440 at 831.798 kb on - strand, within CA265_RS03440 at 831.798 kb on - strand, within CA265_RS03440 at 831.874 kb on - strand, within CA265_RS03440 at 831.874 kb on - strand, within CA265_RS03440 at 831.910 kb on - strand, within CA265_RS03440 at 831.932 kb on + strand, within CA265_RS03440 at 831.932 kb on + strand, within CA265_RS03440 at 832.101 kb on + strand, within CA265_RS03440 at 832.101 kb on + strand, within CA265_RS03440 at 832.102 kb on - strand, within CA265_RS03440 at 832.142 kb on + strand, within CA265_RS03440 at 832.142 kb on + strand, within CA265_RS03440 at 832.142 kb on + strand, within CA265_RS03440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 828,649 - CA265_RS03425 0.39 +1.0 828,651 - CA265_RS03425 0.39 +0.2 828,684 + CA265_RS03425 0.41 +0.2 828,685 - CA265_RS03425 0.41 +0.9 828,685 - CA265_RS03425 0.41 -1.8 828,765 + CA265_RS03425 0.46 -0.1 828,814 + CA265_RS03425 0.49 -0.4 828,837 + CA265_RS03425 0.50 +0.7 828,842 + CA265_RS03425 0.50 -0.3 828,859 + CA265_RS03425 0.51 -1.1 828,859 + CA265_RS03425 0.51 -1.8 828,877 + CA265_RS03425 0.52 -0.7 828,887 - CA265_RS03425 0.53 -0.1 828,892 - CA265_RS03425 0.53 -0.3 828,899 - CA265_RS03425 0.54 -0.4 829,044 + CA265_RS03425 0.62 +0.8 829,178 - CA265_RS03425 0.71 +0.4 829,214 + CA265_RS03425 0.73 +1.3 829,268 + CA265_RS03425 0.76 +1.4 829,268 + CA265_RS03425 0.76 +0.5 829,298 + CA265_RS03425 0.78 -2.0 829,301 + CA265_RS03425 0.78 -0.6 829,301 + CA265_RS03425 0.78 -0.1 829,302 - CA265_RS03425 0.78 +1.3 829,302 - CA265_RS03425 0.78 +1.1 829,302 - CA265_RS03425 0.78 +1.1 829,434 + CA265_RS03425 0.86 -0.7 829,512 + -1.5 829,513 - +1.3 829,538 + -1.2 829,625 - -1.0 829,705 + -0.6 829,705 + +0.8 829,786 - +0.6 829,789 + +0.4 829,794 - CA265_RS03430 0.10 -0.6 829,794 - CA265_RS03430 0.10 -2.0 829,836 + -0.0 829,837 - CA265_RS03430 0.13 +1.1 829,868 + CA265_RS03430 0.15 +0.4 829,869 - CA265_RS03430 0.15 -0.9 829,957 - CA265_RS03430 0.21 +0.2 829,980 + CA265_RS03430 0.22 +1.2 829,990 + CA265_RS03430 0.23 +0.8 829,990 + CA265_RS03430 0.23 -0.7 829,991 - CA265_RS03430 0.23 +0.3 829,991 - CA265_RS03430 0.23 -0.2 829,991 - CA265_RS03430 0.23 -0.5 829,991 - CA265_RS03430 0.23 -0.8 830,025 - CA265_RS03430 0.25 -0.2 830,027 - CA265_RS03430 0.25 -0.0 830,027 - CA265_RS03430 0.25 +1.1 830,134 + CA265_RS03430 0.33 -1.1 830,134 + CA265_RS03430 0.33 -0.1 830,160 + CA265_RS03430 0.34 -0.7 830,221 + CA265_RS03430 0.38 -0.3 830,222 - CA265_RS03430 0.38 -0.1 830,240 - CA265_RS03430 0.40 -1.0 830,245 - CA265_RS03430 0.40 -1.7 830,249 + CA265_RS03430 0.40 +0.6 830,300 - CA265_RS03430 0.44 -0.5 830,464 + CA265_RS03430 0.54 -2.1 830,464 + CA265_RS03430 0.54 +0.4 830,464 + CA265_RS03430 0.54 +0.5 830,465 - CA265_RS03430 0.54 -1.4 830,465 - CA265_RS03430 0.54 +0.3 830,465 - CA265_RS03430 0.54 -0.4 830,480 + CA265_RS03430 0.55 -2.0 830,481 - CA265_RS03430 0.55 +1.2 830,611 + CA265_RS03430 0.64 -0.1 830,612 - CA265_RS03430 0.64 +1.6 830,612 - CA265_RS03430 0.64 -0.0 830,617 + CA265_RS03430 0.64 -2.0 830,779 - CA265_RS03430 0.75 -0.4 830,844 - CA265_RS03430 0.79 -1.8 830,973 + CA265_RS03430 0.88 +1.2 830,973 + CA265_RS03430 0.88 -0.5 830,989 + CA265_RS03430 0.89 +0.0 830,990 - CA265_RS03430 0.89 -0.0 830,990 - CA265_RS03430 0.89 -0.7 830,997 + CA265_RS03430 0.90 -0.7 831,087 - +0.8 831,088 + +1.5 831,089 - -2.0 831,090 + +0.8 831,090 + +0.3 831,090 + +0.4 831,128 - -1.1 831,156 + +1.0 831,178 + CA265_RS03435 0.10 +1.0 831,181 + CA265_RS03435 0.12 +0.9 831,199 + CA265_RS03435 0.20 -0.7 831,200 - CA265_RS03435 0.20 +0.3 831,327 + CA265_RS03435 0.76 -2.7 831,344 - -0.4 831,348 + CA265_RS03435 0.86 +0.6 831,371 + +0.0 831,389 - -2.9 831,404 + -1.8 831,409 - +1.0 831,409 - -0.8 831,428 + -0.5 831,462 - -2.1 831,497 - -0.2 831,511 - +0.2 831,525 + -0.8 831,526 - -0.0 831,562 + -0.1 831,654 - -0.4 831,718 + CA265_RS03440 0.12 -1.4 831,735 + CA265_RS03440 0.13 +1.2 831,755 - CA265_RS03440 0.15 +0.2 831,755 - CA265_RS03440 0.15 +0.2 831,762 + CA265_RS03440 0.15 -1.7 831,798 - CA265_RS03440 0.18 -3.9 831,798 - CA265_RS03440 0.18 +1.8 831,874 - CA265_RS03440 0.24 +1.2 831,874 - CA265_RS03440 0.24 -0.2 831,910 - CA265_RS03440 0.27 -0.7 831,932 + CA265_RS03440 0.28 -3.1 831,932 + CA265_RS03440 0.28 +0.6 832,101 + CA265_RS03440 0.41 +0.9 832,101 + CA265_RS03440 0.41 -3.0 832,102 - CA265_RS03440 0.41 +0.1 832,142 + CA265_RS03440 0.44 -1.5 832,142 + CA265_RS03440 0.44 -2.4 832,142 + CA265_RS03440 0.44 +0.9
Or see this region's nucleotide sequence