Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03065 and CA265_RS03070 are separated by 64 nucleotides CA265_RS03070 and CA265_RS03075 are separated by 9 nucleotides CA265_RS03075 and CA265_RS03080 are separated by 172 nucleotides
CA265_RS03065: CA265_RS03065 - hypothetical protein, at 760,543 to 761,079
_RS03065
CA265_RS03070: CA265_RS03070 - sec-independent protein translocase TatC, at 761,144 to 761,539
_RS03070
CA265_RS03075: CA265_RS03075 - hypothetical protein, at 761,549 to 761,974
_RS03075
CA265_RS03080: CA265_RS03080 - malate dehydrogenase, at 762,147 to 763,085
_RS03080
Position (kb) 761
762 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 760.641 kb on + strand, within CA265_RS03065 at 760.765 kb on + strand, within CA265_RS03065 at 760.777 kb on - strand, within CA265_RS03065 at 760.783 kb on + strand, within CA265_RS03065 at 760.839 kb on + strand, within CA265_RS03065 at 760.840 kb on - strand, within CA265_RS03065 at 760.878 kb on + strand, within CA265_RS03065 at 760.993 kb on - strand, within CA265_RS03065 at 761.001 kb on + strand, within CA265_RS03065 at 761.020 kb on + strand, within CA265_RS03065 at 761.021 kb on - strand, within CA265_RS03065 at 761.099 kb on + strand at 761.236 kb on + strand, within CA265_RS03070 at 761.236 kb on + strand, within CA265_RS03070 at 761.324 kb on + strand, within CA265_RS03070 at 761.357 kb on - strand, within CA265_RS03070 at 761.359 kb on - strand, within CA265_RS03070 at 761.427 kb on - strand, within CA265_RS03070 at 761.447 kb on + strand, within CA265_RS03070 at 761.458 kb on + strand, within CA265_RS03070 at 761.468 kb on - strand, within CA265_RS03070 at 761.492 kb on - strand, within CA265_RS03070 at 761.492 kb on - strand, within CA265_RS03070 at 761.509 kb on + strand at 761.510 kb on - strand at 761.547 kb on + strand at 761.547 kb on + strand at 761.548 kb on - strand at 761.608 kb on + strand, within CA265_RS03075 at 761.609 kb on + strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.626 kb on + strand, within CA265_RS03075 at 761.626 kb on + strand, within CA265_RS03075 at 761.631 kb on - strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.633 kb on - strand, within CA265_RS03075 at 761.785 kb on + strand, within CA265_RS03075 at 761.805 kb on - strand, within CA265_RS03075 at 761.839 kb on + strand, within CA265_RS03075 at 761.851 kb on + strand, within CA265_RS03075 at 761.866 kb on + strand, within CA265_RS03075 at 761.866 kb on + strand, within CA265_RS03075 at 761.979 kb on + strand at 761.979 kb on + strand at 762.023 kb on - strand at 762.048 kb on + strand at 762.057 kb on - strand at 762.067 kb on + strand at 762.067 kb on + strand at 762.068 kb on - strand at 762.068 kb on - strand at 762.068 kb on - strand at 762.093 kb on - strand at 762.137 kb on + strand at 762.137 kb on + strand at 762.182 kb on + strand at 762.275 kb on + strand, within CA265_RS03080 at 762.286 kb on - strand, within CA265_RS03080 at 762.291 kb on + strand, within CA265_RS03080 at 762.300 kb on - strand, within CA265_RS03080 at 762.426 kb on + strand, within CA265_RS03080 at 762.458 kb on + strand, within CA265_RS03080 at 762.483 kb on - strand, within CA265_RS03080 at 762.501 kb on - strand, within CA265_RS03080 at 762.520 kb on + strand, within CA265_RS03080 at 762.555 kb on - strand, within CA265_RS03080 at 762.607 kb on - strand, within CA265_RS03080 at 762.623 kb on - strand, within CA265_RS03080 at 762.706 kb on + strand, within CA265_RS03080 at 762.707 kb on - strand, within CA265_RS03080 at 762.803 kb on + strand, within CA265_RS03080 at 762.806 kb on + strand, within CA265_RS03080 at 762.858 kb on - strand, within CA265_RS03080 at 762.892 kb on + strand, within CA265_RS03080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 760,641 + CA265_RS03065 0.18 +0.1 760,765 + CA265_RS03065 0.41 +0.3 760,777 - CA265_RS03065 0.44 -0.6 760,783 + CA265_RS03065 0.45 +1.0 760,839 + CA265_RS03065 0.55 -1.0 760,840 - CA265_RS03065 0.55 +0.1 760,878 + CA265_RS03065 0.62 +0.7 760,993 - CA265_RS03065 0.84 -0.0 761,001 + CA265_RS03065 0.85 +0.9 761,020 + CA265_RS03065 0.89 +0.6 761,021 - CA265_RS03065 0.89 -1.2 761,099 + -2.3 761,236 + CA265_RS03070 0.23 -1.6 761,236 + CA265_RS03070 0.23 -0.3 761,324 + CA265_RS03070 0.45 -1.2 761,357 - CA265_RS03070 0.54 -0.2 761,359 - CA265_RS03070 0.54 +0.2 761,427 - CA265_RS03070 0.71 +0.0 761,447 + CA265_RS03070 0.77 -1.8 761,458 + CA265_RS03070 0.79 -0.9 761,468 - CA265_RS03070 0.82 -0.8 761,492 - CA265_RS03070 0.88 -0.3 761,492 - CA265_RS03070 0.88 +0.3 761,509 + -2.4 761,510 - +0.3 761,547 + -1.2 761,547 + +1.0 761,548 - +0.0 761,608 + CA265_RS03075 0.14 -0.2 761,609 + CA265_RS03075 0.14 -1.8 761,610 - CA265_RS03075 0.14 -1.1 761,610 - CA265_RS03075 0.14 +2.0 761,610 - CA265_RS03075 0.14 +0.1 761,626 + CA265_RS03075 0.18 +2.0 761,626 + CA265_RS03075 0.18 -1.5 761,631 - CA265_RS03075 0.19 -0.2 761,632 + CA265_RS03075 0.19 -0.1 761,632 + CA265_RS03075 0.19 -0.5 761,632 + CA265_RS03075 0.19 +0.6 761,633 - CA265_RS03075 0.20 -2.4 761,785 + CA265_RS03075 0.55 -2.8 761,805 - CA265_RS03075 0.60 -2.6 761,839 + CA265_RS03075 0.68 +2.4 761,851 + CA265_RS03075 0.71 -0.3 761,866 + CA265_RS03075 0.74 -1.5 761,866 + CA265_RS03075 0.74 -0.6 761,979 + -1.1 761,979 + -1.2 762,023 - -1.2 762,048 + -0.0 762,057 - -0.4 762,067 + +3.0 762,067 + +0.3 762,068 - +0.7 762,068 - -0.3 762,068 - -0.1 762,093 - +0.3 762,137 + +1.1 762,137 + +1.8 762,182 + +0.9 762,275 + CA265_RS03080 0.14 +1.5 762,286 - CA265_RS03080 0.15 +1.6 762,291 + CA265_RS03080 0.15 +1.0 762,300 - CA265_RS03080 0.16 -1.3 762,426 + CA265_RS03080 0.30 +0.9 762,458 + CA265_RS03080 0.33 +0.5 762,483 - CA265_RS03080 0.36 +0.5 762,501 - CA265_RS03080 0.38 +0.3 762,520 + CA265_RS03080 0.40 +0.4 762,555 - CA265_RS03080 0.43 -0.2 762,607 - CA265_RS03080 0.49 -0.0 762,623 - CA265_RS03080 0.51 -0.8 762,706 + CA265_RS03080 0.60 -2.3 762,707 - CA265_RS03080 0.60 -0.2 762,803 + CA265_RS03080 0.70 -0.8 762,806 + CA265_RS03080 0.70 -1.9 762,858 - CA265_RS03080 0.76 -1.1 762,892 + CA265_RS03080 0.79 +1.1
Or see this region's nucleotide sequence