Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01755 and CA265_RS01760 overlap by 14 nucleotides CA265_RS01760 and CA265_RS01765 are separated by 4 nucleotides CA265_RS01765 and CA265_RS01770 overlap by 20 nucleotides
CA265_RS01755: CA265_RS01755 - hypothetical protein, at 441,818 to 443,002
_RS01755
CA265_RS01760: CA265_RS01760 - aminotransferase DegT, at 442,989 to 444,110
_RS01760
CA265_RS01765: CA265_RS01765 - hypothetical protein, at 444,115 to 444,870
_RS01765
CA265_RS01770: CA265_RS01770 - hypothetical protein, at 444,851 to 445,300
_RS01770
Position (kb) 442
443
444
445 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 442.003 kb on - strand, within CA265_RS01755 at 442.030 kb on + strand, within CA265_RS01755 at 442.055 kb on + strand, within CA265_RS01755 at 442.055 kb on + strand, within CA265_RS01755 at 442.055 kb on + strand, within CA265_RS01755 at 442.055 kb on + strand, within CA265_RS01755 at 442.056 kb on - strand, within CA265_RS01755 at 442.074 kb on - strand, within CA265_RS01755 at 442.099 kb on + strand, within CA265_RS01755 at 442.122 kb on - strand, within CA265_RS01755 at 442.138 kb on + strand, within CA265_RS01755 at 442.142 kb on - strand, within CA265_RS01755 at 442.142 kb on - strand, within CA265_RS01755 at 442.142 kb on - strand, within CA265_RS01755 at 442.144 kb on - strand, within CA265_RS01755 at 442.144 kb on - strand, within CA265_RS01755 at 442.144 kb on - strand, within CA265_RS01755 at 442.181 kb on + strand, within CA265_RS01755 at 442.184 kb on + strand, within CA265_RS01755 at 442.229 kb on - strand, within CA265_RS01755 at 442.263 kb on - strand, within CA265_RS01755 at 442.270 kb on + strand, within CA265_RS01755 at 442.321 kb on - strand, within CA265_RS01755 at 442.353 kb on + strand, within CA265_RS01755 at 442.375 kb on + strand, within CA265_RS01755 at 442.479 kb on - strand, within CA265_RS01755 at 442.480 kb on + strand, within CA265_RS01755 at 442.518 kb on - strand, within CA265_RS01755 at 442.521 kb on - strand, within CA265_RS01755 at 442.599 kb on + strand, within CA265_RS01755 at 442.599 kb on + strand, within CA265_RS01755 at 442.600 kb on - strand, within CA265_RS01755 at 442.634 kb on + strand, within CA265_RS01755 at 442.634 kb on + strand, within CA265_RS01755 at 442.680 kb on - strand, within CA265_RS01755 at 442.680 kb on - strand, within CA265_RS01755 at 442.680 kb on - strand, within CA265_RS01755 at 442.682 kb on - strand, within CA265_RS01755 at 442.725 kb on + strand, within CA265_RS01755 at 442.991 kb on - strand at 442.992 kb on + strand at 443.019 kb on + strand at 443.139 kb on + strand, within CA265_RS01760 at 443.196 kb on + strand, within CA265_RS01760 at 443.196 kb on + strand, within CA265_RS01760 at 443.208 kb on - strand, within CA265_RS01760 at 443.335 kb on + strand, within CA265_RS01760 at 443.339 kb on + strand, within CA265_RS01760 at 443.340 kb on - strand, within CA265_RS01760 at 443.340 kb on - strand, within CA265_RS01760 at 443.340 kb on - strand, within CA265_RS01760 at 443.377 kb on - strand, within CA265_RS01760 at 443.394 kb on + strand, within CA265_RS01760 at 443.451 kb on + strand, within CA265_RS01760 at 443.452 kb on - strand, within CA265_RS01760 at 443.468 kb on + strand, within CA265_RS01760 at 443.579 kb on + strand, within CA265_RS01760 at 443.585 kb on + strand, within CA265_RS01760 at 443.689 kb on - strand, within CA265_RS01760 at 443.724 kb on - strand, within CA265_RS01760 at 443.825 kb on + strand, within CA265_RS01760 at 443.825 kb on + strand, within CA265_RS01760 at 443.837 kb on + strand, within CA265_RS01760 at 443.841 kb on + strand, within CA265_RS01760 at 443.977 kb on + strand, within CA265_RS01760 at 443.978 kb on - strand, within CA265_RS01760 at 443.978 kb on - strand, within CA265_RS01760 at 444.009 kb on - strand at 444.045 kb on - strand at 444.054 kb on + strand at 444.054 kb on + strand at 444.057 kb on - strand at 444.241 kb on - strand, within CA265_RS01765 at 444.270 kb on + strand, within CA265_RS01765 at 444.312 kb on + strand, within CA265_RS01765 at 444.313 kb on - strand, within CA265_RS01765 at 444.318 kb on - strand, within CA265_RS01765 at 444.322 kb on - strand, within CA265_RS01765 at 444.459 kb on + strand, within CA265_RS01765 at 444.463 kb on + strand, within CA265_RS01765 at 444.525 kb on + strand, within CA265_RS01765 at 444.525 kb on + strand, within CA265_RS01765 at 444.591 kb on + strand, within CA265_RS01765 at 444.591 kb on + strand, within CA265_RS01765 at 444.591 kb on + strand, within CA265_RS01765 at 444.686 kb on + strand, within CA265_RS01765 at 444.775 kb on - strand, within CA265_RS01765 at 444.786 kb on + strand, within CA265_RS01765 at 444.787 kb on - strand, within CA265_RS01765 at 444.787 kb on - strand, within CA265_RS01765 at 444.788 kb on + strand, within CA265_RS01765 at 444.789 kb on - strand, within CA265_RS01765 at 444.789 kb on - strand, within CA265_RS01765 at 445.093 kb on + strand, within CA265_RS01770 at 445.099 kb on - strand, within CA265_RS01770 at 445.104 kb on - strand, within CA265_RS01770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 442,003 - CA265_RS01755 0.16 -1.3 442,030 + CA265_RS01755 0.18 -0.9 442,055 + CA265_RS01755 0.20 -0.3 442,055 + CA265_RS01755 0.20 -0.1 442,055 + CA265_RS01755 0.20 -1.3 442,055 + CA265_RS01755 0.20 -0.2 442,056 - CA265_RS01755 0.20 -1.7 442,074 - CA265_RS01755 0.22 -1.4 442,099 + CA265_RS01755 0.24 -1.3 442,122 - CA265_RS01755 0.26 -1.0 442,138 + CA265_RS01755 0.27 +0.5 442,142 - CA265_RS01755 0.27 -2.9 442,142 - CA265_RS01755 0.27 +0.3 442,142 - CA265_RS01755 0.27 -2.5 442,144 - CA265_RS01755 0.28 -3.5 442,144 - CA265_RS01755 0.28 -0.8 442,144 - CA265_RS01755 0.28 -0.9 442,181 + CA265_RS01755 0.31 -3.7 442,184 + CA265_RS01755 0.31 -3.0 442,229 - CA265_RS01755 0.35 -0.3 442,263 - CA265_RS01755 0.38 -0.5 442,270 + CA265_RS01755 0.38 +1.5 442,321 - CA265_RS01755 0.42 -3.6 442,353 + CA265_RS01755 0.45 -1.2 442,375 + CA265_RS01755 0.47 -0.1 442,479 - CA265_RS01755 0.56 +0.7 442,480 + CA265_RS01755 0.56 +0.6 442,518 - CA265_RS01755 0.59 -3.6 442,521 - CA265_RS01755 0.59 +0.2 442,599 + CA265_RS01755 0.66 -1.6 442,599 + CA265_RS01755 0.66 -1.2 442,600 - CA265_RS01755 0.66 +0.6 442,634 + CA265_RS01755 0.69 -0.7 442,634 + CA265_RS01755 0.69 -3.4 442,680 - CA265_RS01755 0.73 -0.4 442,680 - CA265_RS01755 0.73 -1.4 442,680 - CA265_RS01755 0.73 -0.7 442,682 - CA265_RS01755 0.73 +0.3 442,725 + CA265_RS01755 0.77 -1.5 442,991 - +0.6 442,992 + +1.2 443,019 + -1.7 443,139 + CA265_RS01760 0.13 -0.7 443,196 + CA265_RS01760 0.18 -0.5 443,196 + CA265_RS01760 0.18 -0.2 443,208 - CA265_RS01760 0.20 -2.8 443,335 + CA265_RS01760 0.31 -1.1 443,339 + CA265_RS01760 0.31 -1.4 443,340 - CA265_RS01760 0.31 -1.8 443,340 - CA265_RS01760 0.31 +0.2 443,340 - CA265_RS01760 0.31 -3.1 443,377 - CA265_RS01760 0.35 -1.3 443,394 + CA265_RS01760 0.36 -2.3 443,451 + CA265_RS01760 0.41 -1.4 443,452 - CA265_RS01760 0.41 -1.0 443,468 + CA265_RS01760 0.43 -0.0 443,579 + CA265_RS01760 0.53 -1.0 443,585 + CA265_RS01760 0.53 -0.5 443,689 - CA265_RS01760 0.62 -0.5 443,724 - CA265_RS01760 0.66 -2.3 443,825 + CA265_RS01760 0.75 -2.7 443,825 + CA265_RS01760 0.75 +0.7 443,837 + CA265_RS01760 0.76 +0.0 443,841 + CA265_RS01760 0.76 -1.3 443,977 + CA265_RS01760 0.88 +0.3 443,978 - CA265_RS01760 0.88 +0.5 443,978 - CA265_RS01760 0.88 -1.6 444,009 - -1.5 444,045 - +1.9 444,054 + +0.2 444,054 + -3.1 444,057 - -1.7 444,241 - CA265_RS01765 0.17 +1.2 444,270 + CA265_RS01765 0.21 -0.8 444,312 + CA265_RS01765 0.26 +1.3 444,313 - CA265_RS01765 0.26 -2.4 444,318 - CA265_RS01765 0.27 -2.7 444,322 - CA265_RS01765 0.27 -3.6 444,459 + CA265_RS01765 0.46 +0.7 444,463 + CA265_RS01765 0.46 -0.8 444,525 + CA265_RS01765 0.54 -1.5 444,525 + CA265_RS01765 0.54 -1.3 444,591 + CA265_RS01765 0.63 -0.5 444,591 + CA265_RS01765 0.63 -0.7 444,591 + CA265_RS01765 0.63 -0.0 444,686 + CA265_RS01765 0.76 -0.3 444,775 - CA265_RS01765 0.87 -0.3 444,786 + CA265_RS01765 0.89 -0.8 444,787 - CA265_RS01765 0.89 -0.9 444,787 - CA265_RS01765 0.89 +0.8 444,788 + CA265_RS01765 0.89 +0.4 444,789 - CA265_RS01765 0.89 +0.8 444,789 - CA265_RS01765 0.89 -0.2 445,093 + CA265_RS01770 0.54 -0.8 445,099 - CA265_RS01770 0.55 -1.4 445,104 - CA265_RS01770 0.56 -0.2
Or see this region's nucleotide sequence